In 1999 a meeting took place at the Jackson Laboratory, a large mouse research centre ...... with European (domesticus) subspecies to produce fancy mice in the ...
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Methods (San Diego, Calif.) Published as: Methods. 2011 February ; 53(2): 163–174.
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Mapping quantitative traits and strategies to find quantitative trait genes Jonathan Flint⁎ Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, UK
Abstract
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In 1999 a meeting took place at the Jackson Laboratory, a large mouse research centre in Bar Harbor, Maine, to consider the value of systematically collecting phenotypes on inbred strains of mice (Paigen and Eppig (2000) [1]). The group concluded that cataloguing the extensive phenotypic diversity present among laboratory mice, and in particular providing the research community with data from cohorts of animals, phenotyped according to standardized protocols, was essential if we were to take advantage of the possibilities of mouse genetics. Beginning with the collection of basic physiological, biochemical and behavioral data on nine commonly used inbred strains, the project has expanded so that by the beginning of 2010 data for 178 strains had been collected, with 105 phenotype projects yielding over 2000 different measurements (Bogue et al. (2007) [2].
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Introduction
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In 1999 a meeting took place at the Jackson Laboratory, a large mouse research centre in Bar Harbor, Maine, to consider the value of systematically collecting phenotypes on inbred strains of mice [1]. The group concluded that cataloguing the extensive phenotypic diversity present among laboratory mice, and in particular providing the research community with data from cohorts of animals, phenotyped according to standardized protocols, was essential if we were to take advantage of the possibilities of mouse genetics. Beginning with the collection of basic physiological, biochemical and behavioral data on nine commonly used inbred strains, the project has expanded so that by the beginning of 2010 data for 178 strains had been collected, with 105 phenotype projects yielding over 2000 different measurements [2]. Fig. 1 provides a representative example, in this case data for 16 strains for three haematological phenotypes (red blood cell, platelet and white blood cell counts). The distribution of each strain phenotype is shown as a box and whisker plot, from which it is immediately clear that there is large and highly significant variation between strains (for example an analysis of variance for the red blood cell phenotype gives an F value of 56.1 (on 15 and 381 degrees of freedom), with a P-value of
