Document not found! Please try again

microRNA expression signatures cell populations ...

4 downloads 0 Views 1016KB Size Report
Mar 28, 2008 - global gene and microRNA (miR) expres- sion microarrays. ... T-, and mast cell precursors, whereas. G-CSF–mobilized ...... Cytochrome P450, family 3, subfamily A, polypeptide 7. 3.59. 1.45. 10 5 ..... hsa-mir-612. 1.62 .0353.
From bloodjournal.hematologylibrary.org by guest on May 18, 2011. For personal use only.

2009 114: 2530-2541 Prepublished online July 14, 2009; doi:10.1182/blood-2009-04-214403

Plerixafor (AMD3100) and granulocyte colony-stimulating factor (G-CSF) mobilize different CD34 + cell populations based on global gene and microRNA expression signatures Robert E. Donahue, Ping Jin, Aylin C. Bonifacino, Mark E. Metzger, Jiaqiang Ren, Ena Wang and David F. Stroncek

Updated information and services can be found at: http://bloodjournal.hematologylibrary.org/content/114/12/2530.full.html Articles on similar topics can be found in the following Blood collections Hematopoiesis and Stem Cells (2834 articles) Transfusion Medicine (215 articles) Transplantation (1630 articles) Free Research Articles (1134 articles) Information about reproducing this article in parts or in its entirety may be found online at: http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#repub_requests Information about ordering reprints may be found online at: http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#reprints Information about subscriptions and ASH membership may be found online at: http://bloodjournal.hematologylibrary.org/site/subscriptions/index.xhtml

Blood (print ISSN 0006-4971, online ISSN 1528-0020), is published weekly by the American Society of Hematology, 2021 L St, NW, Suite 900, Washington DC 20036. Copyright 2011 by The American Society of Hematology; all rights reserved.

From bloodjournal.hematologylibrary.org by guest on May 18, 2011. For personal use only. TRANSPLANTATION

Plerixafor (AMD3100) and granulocyte colony-stimulating factor (G-CSF) mobilize different CD34⫹ cell populations based on global gene and microRNA expression signatures Robert E. Donahue,1 Ping Jin,2 Aylin C. Bonifacino,1 Mark E. Metzger,1 Jiaqiang Ren,2 Ena Wang,2 and David F. Stroncek2 1Hematology Branch, National Heart, Lung, and Blood Institute; and 2Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD

Plerixafor (AMD3100) and granulocyte colony-stimulating factor (G-CSF) mobilize peripheral blood stem cells by different mechanisms. A rhesus macaque model was used to compare plerixafor and G-CSF–mobilized CD34ⴙ cells. Three peripheral blood stem cell concentrates were collected from 3 macaques treated with G-CSF, plerixafor, or plerixafor plus G-CSF. CD34ⴙ cells were isolated by immunoselection and were analyzed by global gene and microRNA (miR) expression microarrays. Unsupervised hierarchi-

cal clustering of the gene expression data separated the CD34ⴙ cells into 3 groups based on mobilization regimen. Plerixaformobilized cells were enriched for B cells, T cells, and mast cell genes, and G-CSF– mobilized cells were enriched for neutrophils and mononuclear phagocyte genes. Genes up-regulated in plerixafor plus G-CSF–mobilized CD34ⴙ cells included many that were not up-regulated by either agent alone. Two hematopoietic progenitor cell miR, miR-10 and miR-126, and a dendritic cell miR, miR-155, were up-

regulated in G-CSF–mobilized CD34ⴙ cells. A pre-B-cell acute lymphocytic leukemia miR, miR-143-3p, and a T-cell miR, miR-143-5p, were up-regulated in plerixafor plus G-CSF–mobilized cells. The composition of CD34ⴙ cells is dependent on the mobilization protocol. Plerixaformobilized CD34ⴙ cells include more B-, T-, and mast cell precursors, whereas G-CSF–mobilized cells have more neutrophil and mononuclear phagocyte precursors. (Blood. 2009;114:2530-2541)

Introduction Granulocyte colony-stimulating factor (G-CSF) has been the standard hematopoietic growth factor for increasing the levels for circulating hematopoietic stem cells (HSCs) in both healthy subjects donating cells for allogeneic transplantation and patients donating cells for autologous transplantation. G-CSF–mobilized HSCs collected by apheresis have been used for transplantation, immune therapy, and the treatment of cardiac ischemia. Another HSC-mobilizing agent, plerixafor (AMD3100), has been used in large animal models,1,2 with G-CSF to mobilize stem cells for autologous transplants3 and is currently being evaluated as a single agent to mobilize HSCs for allogeneic donor transplants.4 The mechanisms by which plerixafor and G-CSF alter HSC trafficking and mobilize HSCs are different, suggesting that HSCs with different intrinsic properties may be mobilized by these agents. Plerixafor, as a CXC chemokine receptor 4 (CXCR4) antagonist, mobilizes HSCs within 6 hours5-7 by disrupting the engagement of stem cell surface CXCR48,9 with its ligand SDF-1 (CXCL12), which is expressed on marrow osteoblasts.10,11 In contrast, G-CSF mobilizes stem cells indirectly by downregulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrade important HSC-trafficking and adhesion molecules c-kit, VCAM-1, and CXCR4.12 Animal models suggest that plerixafor mobilizes a CD34⫹ cell population different from that mobilized by G-CSF,2,13,14 due to differences in mechanisms of mobilization.

To explore differences between plerixafor and G-CSF mobilization, we used a rhesus macaque HSC-mobilization model to compare CD34⫹ cells mobilized by G-CSF and plerixafor. We also compared CD34⫹ cells mobilized with G-CSF and plerixafor in combination. The combination of G-CSF plus plerixafor was tested because it is being used clinically, and when these agents are used together, more CD34⫹ cells are mobilized than when either agent is used alone.3,15 The CD34⫹ cells were compared using global gene and microRNA expression analysis.

Submitted April 13, 2009; accepted June 24, 2009. Prepublished online as Blood First Edition paper, July 14, 2009; DOI 10.1182/blood-2009-04-214403.

payment. Therefore, and solely to indicate this fact, this article is hereby marked ‘‘advertisement’’ in accordance with 18 USC section 1734.

Methods Study design Young rhesus macaques (Macaca mulatta) were studied before and after the completion of 3 different mobilization protocols. The protocols involved the administration of 5 days of G-CSF, one dose of plerixafor, and 5 days of G-CSF plus one dose of plerixafor. Peripheral blood stem cell (PBSC) concentrates were collected by apheresis after the mobilization protocol was complete. Three rhesus macaques were given all 3 mobilization protocols, and PBSCs were collected from each of these 3 animals. Each course of PBSC mobilization was separated by at least 6 weeks. Two monkeys were given plerixafor first, one was given G-CSF first, and all 3 were given plerixafor plus G-CSF last (Table 1). The CD34⫹ cells were isolated from the PBSCs by using CD34 monoclonal antibodies and magnetic particles. The CD34⫹ cells were analyzed using cDNA gene and miRNA expression microarrays.

The publication costs of this article were defrayed in part by page charge

2530

BLOOD, 17 SEPTEMBER 2009 䡠 VOLUME 114, NUMBER 12

From bloodjournal.hematologylibrary.org by guest on May 18, 2011. For personal use only. BLOOD, 17 SEPTEMBER 2009 䡠 VOLUME 114, NUMBER 12

PLERIXAFOR- AND G-CSF–MOBILIZED CD34⫹ CELLS

2531

Table 1. Composition of PBSC products collected from 3 rhesus macaque and the yield of CD34ⴙ cells isolated from each product Isolated CD34ⴙ cells

PBSC products Mobilizing agents

Mononuclear cells/mL, ⴛ109

Granulocytes/mL, ⴛ109

Animal ID

Collection date

RQ4766

3/28/2008

49

163

1.5 ⫻ 107

86.5

2RC104

1/22/2008

90

81

2.5 ⫻ 107

98.8

RQ4963

1/23/2008

30

184

2.0 ⫻ 107

96.4

56 ⫾ 31

143 ⫾ 54

2.0 ⫾ 0.5 ⫻ 107

93.9 ⫾ 6.5

Total no.

Purity (%)

G-CSF

Mean ⫾ SD Plerixafor RQ4766

11/14/2007

103

30

1.5 ⫻ 107

82.0

2RC104

3/11/2008

99

28

2.6 ⫻ 107

95.1

RQ4963

10/31/2007

107

61

2.0 ⫻ 107

82.0

103 ⫾ 4

40 ⫾ 18

2.0 ⫾ 0.6 ⫻ 107

86.4 ⫾ 7.6

Mean ⫾ SD Plerixafor ⴙ G-CSF RQ4766

7/14/2008

63

215

8.9 ⫻ 107

78.8

2RC104

7/12/2008

56

215

12.5 ⫻ 107

99.5

RQ4963

7/16/2008

55

266

6.4 ⫻ 107

85.5

58 ⫾ 4

233 ⫾ 29

9.3 ⫾ 3.1 ⫻ 107

88.0 ⫾ 10.6

Mean ⫾ SD

Mobilization and collection of PBSC concentrates CD34⫹ cells were mobilized, and PBSC concentrates were collected from rhesus macaques that were housed and handled in accordance with the guidelines set by the Committee on Care and Use of Laboratory Animals of the Institute of Laboratory Animal Resources, National Research Council (DHHS publication no. NIH 85-23). The protocol was approved by the Animal Care and Use Committee of the National Heart, Lung, and Blood Institute, National Institutes of Health. G-CSF mobilization involved the administration of 10 ␮g/kg G-CSF (Filgrastim; Amgen) subcutaneously daily for 5 days. When plerixafor

CD34+

(Genzyme) was used to mobilize peripheral blood mononuclear cells (PBMCs), a single dose of 1 mg/kg plerixafor was given subcutaneously, and when both G-CSF and plerixafor were given, 10 ␮g/kg G-CSF was given for 5 days and 1 mg/kg plerixafor was given on the fifth day. PBSC concentrates were collected by apheresis approximately 2 hours after the last dose of G-CSF or plerixafor. The PBSCs were collected using a CS3000 plus blood cell separator (Baxter Healthcare Corp, Fenwal Division).16 The concentrates were processed to remove contaminating red blood cells and granulocytes by using Ficoll gradient separation. CD34⫹ cells were isolated by immunomagnetic separation as previously described.16 Purification was determined following immunoselection using phycoerythrin-conjugated murine anti-rhesus/human CD34 antibody (clone 563; BD Biosciences), which recognizes a different epitope from that used in the immunoselection procedure. The processed CD34⫹ cells were frozen and stored at ⫺80°C until analysis.

Plerixafor + G-CSF G-CSF

CD34-

Figure 1. Comparison of gene expression profiles of CD34ⴙ cells mobilized with plerixafor, G-CSF, and plerixafor plus G-CSF with PBMCs. CD34⫹ cells were isolated by immunomagnetic selection from plerixafor, G-CSF, and plerixafor plus G-CSF– mobilized PBSCs collected by apheresis. The CD34⫹ cells and unabsorbed PBMCs were analyzed by gene expression profiling using a cDNA microarray with more than 17 000 genes. The 5378 genes that were expressed in at least 80% of the samples and were increased at least 2-fold in one sample were analyzed by hierarchical cluster of Eisen. G indicates G-CSF; A, plerixafor; A⫹G, plerixafor plus G-CSF.

Plerixafor

Figure 2. Unsupervised hierarchical clustering analysis of gene differentially expressed among CD34ⴙ cells mobilized plerixafor, G-CSF, and plerixafor plus G-CSF in 3 rhesus macaques. The CD34⫹ cells were analyzed by gene expression profiling using a cDNA microarray with more than 17 000 genes. Among 5378 genes that were expressed in at least 80% of the samples and were increased at least 2-fold, 1097 were found to be differentially expressed in 3 clusters of CD34⫹ cells (F test, P ⱕ .005). The differentially expressed genes were analyzed by hierarchical cluster of Eisen. G indicates G-CSF; A, plerixafor; A⫹G, plerixafor plus G-CSF.

From bloodjournal.hematologylibrary.org by guest on May 18, 2011. For personal use only. 2532

BLOOD, 17 SEPTEMBER 2009 䡠 VOLUME 114, NUMBER 12

DONAHUE et al

Table 2. KEGG pathways with the most genes up-regulated in G-CSF–mobilized CD34ⴙ cells and plerixafor-mobilized CD34ⴙ cells Pathways with genes up-regulated by G-CSF Pathway

Pathways with genes up-regulated by plerixafor

No. of genes in pathway

Pathway

No. of genes in pathway

Cytokine-cytokine receptor interaction

30

Cytokine-cytokine receptor interaction

Jak-STAT signaling pathway

25

Calcium signaling pathway

29 27

MAPK signaling pathway

24

T-cell receptor signaling pathway

27

Focal adhesion

22

Hematopoietic cell lineage

24

Insulin signaling pathway

21

Wnt signaling pathway

22

Hematopoietic cell lineage

20

Primary immunodeficiency

21

Natural killer cell–mediated cytotoxicity

19

Cell cycle

20

Purine metabolism

19

MAPK signaling pathway

20

ErbB signaling pathway

16

Focal adhesion

19 19

VEGF signaling pathway

15

Natural killer cell mediated cytotoxicity

Regulation of actin cytoskeleton

14

TGF-beta signaling pathway

19

Toll-like receptor signaling pathway

14

Fc epsilon RI signaling pathway

18 18

Tyrosine metabolism

14

Jak-STAT signaling pathway

Acute myeloid leukemia

13

GnRH signaling pathway

15

Fc epsilon RI signaling pathway

13

Cell adhesion molecules

14

GnRH signaling pathway

13

Tight junction

13

Neuroactive ligand-receptor interaction

13

Adherens junction

12

Wnt signaling pathway

13

Antigen processing and presentation

12

Calcium signaling pathway

12

B-cell receptor signaling pathway

12

Complement and coagulation cascades

12

Neuroactive ligand-receptor interaction

12

Fructose and mannose metabolism

12

Gap junction

11

Glycine, serine, and threonine metabolism

12

Long-term depression

11

Leukocyte transendothelial migration

12

Melanogenesis

11

Melanogenesis

12

Regulation of actin cytoskeleton

11

Analysis of gene expression with a 17 500 gene cDNA microarray Total RNA was extracted from the rhesus samples and amplified into antisense RNA (aRNA). In addition, total RNA from human PBMCs pooled from 6 healthy subjects was extracted and amplified into aRNA

to serve as a constant reference. Test and reference RNA were labeled with Cy5 (red) and Cy3 (green), respectively, and cohybridized to a custom-made 17.5K cDNA (UniGene cluster) microarray. The microarrays were printed in the Immunogenetics Section, Department of Transfusion Medicine (DTM), Clinical Center (CC), National Institutes

Table 3. Genes whose expression was increased the greatest in G-CSF–mobilized CD34ⴙ cells compared with plerixafor-mobilized CD34ⴙ cells Gene

Fold increase*

P

9.04

5.30 ⫻ 10⫺6

NCF4

Neutrophil cytosolic factor 4, 40 kDa

VNN2

Vanin 2

7.57

7.90 ⫻ 10⫺6

PRTN3

Proteinase 3 (serine proteinase, neutrophil, Wegener granulomatosis autoantigen)

7.25

4.49 ⫻ 10⫺4

LTBR

Lymphotoxin-beta receptor precursor ⫽ tumor necrosis factor receptor 2 related protein ⫽ tumor necrosis

7.23

1.33 ⫻ 10⫺4

factor C receptor CST7

Cystatin F (leukocystatin) (inhibits cathepsins)

7.05

9.00 ⫻ 10⫺6

FCGR3A

CD16 ⫽ Fc-gamma receptor IIIa

7.00

2.60 ⫻ 10⫺5

CTSL

Cathepsin L

6.50

8.00 ⫻ 10⫺7

ANXA3

Annexin A3

6.17

1.17 ⫻ 10⫺4

CST7

Cystatin F (leukocystatin)

5.83

5.80 ⫻ 10⫺6

TSPYL2

TSPY-like 2

4.73

8.58 ⫻ 10⫺4

HSPA1B

Heat shock 70-kDa protein 1B

4.67

.012

ANXA3

Annexin A3

4.64

8.42 ⫻ 10⫺5

DYSF

Dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive)

4.65

1.47 ⫻ 10⫺3

CLU

Clusterin

4.51

8.50 ⫻ 10⫺6

MYCO

Myocilin, trabecular meshwork inducible glucocorticoid response

4.23

1.20 ⫻ 10⫺3

PPBP

CTAP-III ⫽ NAP2 ⫽ connective tissue activation peptide III ⫽ pro-platelet basic protein

4.09

6.47 ⫻ 10⫺3

C1QB

Complement component 1, q subcomponent, B chain

4.00

4.14 ⫻ 10⫺3

RNASE2

Ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin)

3.99

7.57 ⫻ 10⫺4

GOS2

putative lymphocyte G0/G1 switch gene

3.96

8.50 ⫻ 10⫺5

TNFRSF1

Tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)

3.84

3.72 ⫻ 10⫺4

CXCL2

Chemokine (C-X-C motif) ligand

3.65

6.73 ⫻ 10⫺4

FCGR2B

Low-affinity IgG Fc receptor II-B and C isoforms

3.61

1.98 ⫻ 10⫺4

IL21R

Interleukin-21 receptor

3.55

3.12 ⫻ 10⫺4

CX3CR1

Chemokine (C-X3-C motif) receptor 1

3.52

1.20 ⫻ 10⫺4

SLCIA5

Solute carrier family 1 (neutral amino acid transporter), member 5

3.35

2.43 ⫻ 10⫺4

PTRF

Polymerase I and transcript release factor

3.28

3.92 ⫻ 10⫺5

*The fold increase for each gene is the average expression of each gene in G-CSF–mobilized CD34⫹ cells from 3 rhesus macaque divided by the average expression of the same gene in plerixafor-mobilized CD34⫹ cells from 3 monkeys.

From bloodjournal.hematologylibrary.org by guest on May 18, 2011. For personal use only. BLOOD, 17 SEPTEMBER 2009 䡠 VOLUME 114, NUMBER 12

PLERIXAFOR- AND G-CSF–MOBILIZED CD34⫹ CELLS

2533

Figure 3. Analysis of differentially expressed mobilized CD34ⴙ cells genes by qRT-PCR. The expression of 5 genes (FLT3, HOXA9, PTGS2, CXCR4, and CTSL) in CD34⫹ cells from the 3 rhesus macaque mobilized by plerixafor (A), G-CSF (G), or plerixafor plus G-CSF (A⫹G) were analyzed by qRT-PCR. The expression of CXCR4 was greater in plerixafor and plerixafor plus G-CSF–mobilized CD34⫹ cells. The expression of CTSL was greater on G-CSF– and G-CSF plus plerixafor-mobilized CD34⫹ cells. FLT3 expression was greatest on plerixafor-mobilized CD34⫹ cells, and the expression of PTGS2 was greatest on plerixafor plus G-CSF–mobilized cells. The level of expression of HOXA9 by the 3 types of CD34⫹ cells was similar but much greater than peripheral blood leukocytes. The expression of the 5 genes in leukocytes that were not absorbed by anti-CD34 from the 9 mobilized PBSC concentrates (CD34⫺) are shown as a control.

of Health with a configuration of 32 ⫻ 24 ⫻ 23 and contained 17 500 elements. The clones used for printing the arrays included a combination of the Research Genetics RG_HsKG_031901 8k clone set and 9000 clones selected from the RG_Hs_seq_ver_070700 40k clone set. The 17 500 spots included 12 072 uniquely named genes, 875 duplicated genes, and approximately 4000 expression sequence tags. A complete list of genes included in the Hs-CCDTM-17.5k-1px printing is available at http://nciarray.nci.nih.gov/gal_files/index.shtml.

Analysis of miRNA expression with a microarray Two ␮g total RNA from each sample were labeled with Hy5 and the reference sample (total RNA from Epstein-Barr virus-transformed lymphoblastoid cell lines) with Hy3 by using a miRCURY LNA Array Power Labeling Kit (Exiqon) following the recommended protocol by the manufacturer’s instruction. A commercially available miRNA probe set with 827 human, mouse, and Epstein-Barr virus miRNA was purchased from Ambion and printed onto the glass slides in the Immunogenetics Section of DTM. A complete list of miR included in our microRNA assay is available at http://nciarray.nci.nih.gov/gal_files/CCDTM-miRNA700-V3px-

A.gal. After labeling, the sample and reference were cohybridized to the miRNA array at room temperature overnight. Both the processed cDNA and the miRNA array slides were scanned by using GenePix Pro 4.0 (Axon). Microarray data have been deposited with Gene Expression Omnibus under accession number GSE16936.

Data and statistical analysis For analysis of the cDNA and miRNA array data, the raw dataset was filtered according to a standard procedure to exclude spots with minimum intensity. This filtering was arbitrarily set to an intensity parameter of 300 for cDNA expression data and 100 for miRNA array data in both fluorescence channels. Spots flagged by the analysis software and/or spots with diameters less than 25 ␮m for cDNA expression array and less than 10 ␮m for the miRNA array were excluded from the analysis. The filtered data were normalized using Lowess Smoother and were retrieved by the BRB ArrayTool (http://linus.nci.nih.gov/BRB-ArrayTools. html) developed at the National Cancer Institute, Biometric Research Branch, Division of Cancer Treatment and Diagnosis. Hierarchical cluster

From bloodjournal.hematologylibrary.org by guest on May 18, 2011. For personal use only. 2534

BLOOD, 17 SEPTEMBER 2009 䡠 VOLUME 114, NUMBER 12

DONAHUE et al

Table 4. Genes whose expression was increased the greatest in plerixafor-mobilized CD34ⴙ cells compared with G-CSF–mobilized CD34ⴙ cells Gene

Fold increase*

P

ICSBP1

Interferon consensus sequence binding protein 1

6.14

9.20 ⫻ 10⫺6

BLNK

B-cell linker

5.99

1.30 ⫻ 10⫺6

CXCR4

CXC chemokine receptor 4

5.17

1.17 ⫻ 10⫺5

CXCR4

CXC chemokine receptor 4

5.07

4.80 ⫻ 10⫺6

CDK5R1

Cyclin-dependent kinase 5, regulatory subunit 1 (p35)

4.62

3.80 ⫻ 10⫺6

TP53I11

Tumor protein p53 inducible protein 11

4.35

8.40 ⫻ 10⫺6

SMAD7

Homologue of Mothers Against Decapentaplegic (MAD) ⫽ negative regulator of TGF-beta signaling

4.19

8.90 ⫻ 10⫺6

FLT3

Fms-related tyrosine kinase 3

3.44

2.05 ⫻ 10⫺6

CXCR4

CXC chemokine receptor 4

3.32

2.49 ⫻ 10⫺4

LDB2

LIM domain binding 2

3.23

3.24 ⫻ 10⫺4

COG1

Component of oligomeric golgi complex 1

3.12

3.15 ⫻ 10⫺5

DCN

Decorin

3.04

5.96 ⫻ 10⫺5

IL23A

Enhancer of polycomb homolog 1 (Drosophila)

2.95

3.58 ⫻ 10⫺3

AGTRL1

Angiotensin II receptor-like 1

2.92

4.68 ⫻ 10⫺4

SPIB

Spi-B transcription factor

2.91

5.17 ⫻ 10⫺4

EBI2

Epstein-Barr virus–induced gene 2 (lymphocyte-specific G protein–coupled receptor)

2.90

1.81 ⫻ 10⫺4

IL7R

Interleukin-7 receptor

2.86

1.81 ⫻ 10⫺4

TRAT1

T-cell receptor–associated transmembrane adaptor 1

2.83

.014

GAP43

Growth associated protein 43

2.82

7.42 ⫻ 10⫺4

CD79A

CD79A, BCR alpha chain ⫽ mb-1

2.95

3.58 ⫻ 10⫺3

TIEG

TGF-beta inducible early protein

2.75

1.84 ⫻ 10⫺4

IL18R1

Interleukin-18 receptor 1

2.73

8.62 ⫻ 10⫺3

SMARCD

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2

2.73

2.73 ⫻ 10⫺3

BTK

Btk ⫽ Bruton agammaglobulinemia tyrosine kinase

2.72

2.67 ⫻ 10⫺3

FCER1A

Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide

2.67

.037

KHDRBS3

KH domain containing, RNA binding, signal transduction associated 3

2.63

3.46 ⫻ 10⫺3

*The fold increase for each gene is the average expression of each gene in plerixafor-mobilized CD34⫹ cells from 3 rhesus macaque divided by the average expression of the same gene in G-CSF–mobilized CD34⫹ cells from 3 monkeys.

analysis was conducted on the genes or miRNA by using Cluster and TreeView software.17 The relationships between the different groups of mobilized CD34⫹ cells were tested by applying unsupervised the Eisen hierarchical clustering method.17 For annotation of genes and functional pathways, the Database for Annotation, Visualization, and Integrated Discovery (DAVID) 2007 software (http://david.abcc.ncifcrf.gov/)18 and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway (http://www.genome.ad.jp/kegg) were used. All target prediction analysis used BRB ArrayTool microRNA targets program (http://linus.nci.nih.gov/BRB-ArrayTools.html), TargetScan (http://www.targetscan.org), and miRBase Targets (http://microrna.sanger.ac.uk).

Results PBSC mobilization and collection

The greatest quantities of CD34⫹ cells were isolated from plerixafor plus G-CSF–mobilized PBMC concentrates (Table 1). Similar quantities of CD34⫹ cells were isolated from G-CSF– mobilized and plerixafor-mobilized PBSC concentrates. Interestingly, the composition of the products differed with more mononuclear cells appearing in the plerixafor-mobilized products than either the G-CSF–mobilized or plerixafor plus G-CSF– mobilized products. Gene expression analysis

Mobilized CD34⫹ cells and the peripheral blood leukocytes that were not absorbed with anti-CD34 were analyzed by gene expression profiling using a microarray with 17 500 cDNA probes. The 5378 genes that were expressed in 80% of these samples and whose expression was increased 2-fold or more in

at least one sample were analyzed by unsupervised hierarchical clustering of Eisen (Figure 1). The samples clustered into 2 major groups: the CD34⫹ group, which included all 9 CD34⫹ cell samples, and the peripheral blood leukocyte group, which included all 9 unabsorbed leukocyte samples. Within the CD34⫹ cell group, a large cluster of genes were expressed at similar levels among all CD34⫹ cell samples. The expression of a second cluster of genes, however, differed among CD34⫹ cells. In fact, the CD34⫹ group was made up of 3 separate clusters: one group that contained all 3 G-CSF–mobilized CD34⫹ cell samples and one plerixafor-mobilized CD34⫹ cell sample, one group that contained 2 plerixafor-mobilized CD34⫹ cell samples, and one that contained all 3 plerixafor plus G-CSF–mobilized CD34⫹ cells samples. These results suggest that CD34⫹ cells mobilized by G-CSF alone and plerixafor alone differ and CD34⫹ cells mobilized by the combination of plerixafor plus G-CSF differed from those mobilized by either G-CSF alone or by plerixafor alone. To further explore the differences among the 3 types of CD34⫹ cells, we identify 1097 genes that were differentially expressed among the 3 types of CD34⫹ cells (F tests, P ⬍ .005). Hierarchical clustering analysis of the differentially expressedgenes separated the CD34⫹ cells into 3 clusters: one with all 3 G-CSF–mobilized samples, one with all 3 plerixaformobilized samples, and one with all 3 plerixafor plus G-CSF– mobilized samples (Figure 2). Although the CD34⫹ cells samples were clustered into 3 separate groups, according to mobilization protocol, the transcription profiles of the plerixafor plus G-CSF–mobilized CD34⫹ cells were not simply a mixture of genes differently expressed by G-CSF–mobilized and plerixafor-mobilized cells. Although some genes that were upregulated in plerixafor plus G-CSF–mobilized CD34⫹ cells

From bloodjournal.hematologylibrary.org by guest on May 18, 2011. For personal use only. BLOOD, 17 SEPTEMBER 2009 䡠 VOLUME 114, NUMBER 12

PLERIXAFOR- AND G-CSF–MOBILIZED CD34⫹ CELLS

2535

Table 5. KEGG pathways with the most genes up-regulated and down-regulated in CD34ⴙ cells mobilized by plerixafor plus G-CSF compared with those mobilized by plerixafor alone and G-CSF alone Pathways with genes up-regulated by plerixafor plus G-CSF Pathway

Pathways with genes down-regulated by plerixafor plus G-CSF

No. of up-regulated genes in pathway

Pathway

No. of down-regulated genes in pathway

Cytokine-cytokine receptor interaction

68

MAPK signaling pathway

MAPK signaling pathway

63

Focal adhesion

51 46

Natural killer cell–mediated cytotoxicity

41

Oxidative phosphorylation

40

Jak-STAT signaling pathway

39

Calcium signaling pathway

37

Focal adhesion

33

Ribosome

36

Leukocyte transendothelial migration

33

Cytokine-cytokine receptor interaction

33

Regulation of actin cytoskeleton

32

Wnt signaling pathway

33

Insulin signaling pathway

29

Tight junction

31

Cell cycle

28

Jak-STAT signaling pathway

29

Gap junction

28

Cell adhesion molecules

28

Calcium signaling pathway

25

Natural killer cell–mediated cytotoxicity

28

Fc epsilon RI signaling pathway

25

Insulin signaling pathway

27

Oxidative phosphorylation

25

Axon guidance

26

Ubiquitin mediated proteolysis

25

ErbB signaling pathway

25

T-cell receptor signaling pathway

24

Small cell lung cancer

25

B-cell receptor signaling pathway

22

Antigen processing and presentation

23

Cell adhesion molecules

22

Leukocyte transendothelial migration

23

Toll-like receptor signaling pathway

22

Gap junction

22

Wnt signaling pathway

22

Hematopoietic cell lineage

22

GnRH signaling pathway

21

Long-term depression

22

Melanogenesis

21

Regulation of actin cytoskeleton

22 22

Tight junction

21

VEGF signaling pathway

Apoptosis

20

Colorectal cancer

21

Hematopoietic cell lineage

20

T-cell receptor signaling pathway

21

Pathogenic Escherichia coli infection—EHEC

20

Ubiquitin mediated proteolysis

21

were also up-regulated in plerixafor-mobilized or G-CSF– mobilized CD34⫹ cells, many genes were only up-regulated in plerixafor plus G-CSF–mobilized CD34⫹ cells (Figure 2). Similarly, many genes were only down-regulated in plerixafor plus G-CSF–mobilized CD34⫹ cells (Figure 2). These results suggest that G-CSF, plerixafor, and the combination of plerixafor plus G-CSF mobilized 3 biologically distinct types of HSCs. Comparison of gene expression in G-CSF– and plerixafor-mobilized CD34ⴙ cells

Student t tests were used to identify genes that were differentially expressed between G-CSF–mobilized CD34⫹ cells and plerixafor-mobilized CD34⫹ cells. The expression of 593 genes was greater in G-CSF–mobilized CD34⫹ cells than plerixaformobilized CD34⫹ cells, and the expression of 618 was greater in plerixafor-mobilized CD34⫹ cells (P ⬍ .05). Genes in the complement and coagulation cascades, Toll-like receptor signaling, ErbB signaling, acute myeloid leukemia, vascular endothelial growth factor signaling, and several metabolism and biosynthesis pathways were more likely to be up-regulated in G-CSF– mobilized CD34⫹ cells (Table 2). Up-regulated genes in plerixafor-mobilized CD34⫹ cells were more likely to include those in the T-cell receptor, primary immunodeficiency, TGFbeta signaling pathway, cell cycle, B-cell receptor, antigen presentation, and cell adhesion molecules signaling pathways. Genes in several pathways were up-regulated in both G-CSF– and plerixafor-mobilized CD34⫹ cells, including cytokinecytokine interaction, Jak-STAT signaling pathway, MAPK signaling pathway, hematopoietic cell lineage, natural killer cell (NK)–mediated cytotoxicity, Fc epsilon RI signaling, Wnt signaling, focal adhesion, and neuroactive ligand-receptor interaction (Table 2).

Comparison of the specific genes up-regulated in G-CSF– mobilized and plerixafor-mobilized CD34⫹ cells revealed that the genes maximally up-regulated by G-CSF included the neutrophil genes, neutrophil cytosolic factor 4 (NCF4) and proteinase 3 (PR3), and the NK-cell gene Fc-gamma receptor IIIa (CD16a; Table 3). G-CSF–mobilized CD34⫹ cells were also more likely to express mononuclear phagocyte genes CX3CR1, cathepsin L (CTSL), and lymphotoxin B receptor precursor. Genes expressed by several types of leukocytes that were up-regulated in G-CSF– mobilized CD34⫹ cells included Fc-gamma receptor IIb, which is expressed by neutrophils and B cells; cystatin F, which is expressed by NK cells and mononuclear phagocytes; and IL-21 receptor, which is expressed by T cells, B cells, NK cells, and mononuclear phagocytes. Analysis of the expression of CTSL by quantitative real-time polymerase chain reaction (qRT-PCR) confirmed that CTSL expression was greater in G-CSF–mobilized CD34⫹ cells than in plerixafor-mobilized CD34⫹ cells (Figure 3). The level of CTSL expressed by plerixafor plus G-CSF– and G-CSF–mobilized CD34⫹ cells were similar. The genes differentially up-regulated most by plerixafor included several copies of CXCR4, which is expressed by hematopoietic stem cells, T cells, B cells, monocytes, and neutrophils. Other plerixafor up-regulated genes included the B-cell genes CD79A, interleukin-7 receptor, Epstein-Barr virus–induced gene 2, and Bruton agammaglobulinemia tyrosine kinase; a T-cell gene, T-cell receptor–associated transmembrane adaptor 1; and a mast cell gene, Fc receptor of IgE 1a (Table 4). In addition, the expression of the HSC marker Fms-related tyrosine kinase 3 (FLT3) was up-regulated in plerixafor-mobilized CD34⫹ cells. Analysis of CXCR4 and FLT3 expression by qRT-PCR confirmed that the expression of both was greater in plerixafor-mobilized CD34⫹ cells than G-CSF– mobilized CD34⫹ cells (Figure 3). These results suggest that G-CSF–

From bloodjournal.hematologylibrary.org by guest on May 18, 2011. For personal use only. 2536

BLOOD, 17 SEPTEMBER 2009 䡠 VOLUME 114, NUMBER 12

DONAHUE et al

Table 6. Genes whose expression was increased the greatest in plerixafor plus G-CSF–mobilized CD34ⴙ cells compared with those mobilized by plerixafor alone and G-CSF alone Gene

Description

Fold increase*

Parametric P 2.45 ⫻ 10⫺4

MGC27165

Immunoglobulin alpha (1 or 2) heavy chain constant region

9.04

BTK

btk ⫽ Bruton agammaglobulinemia tyrosine kinase

5.13

⬍ 1 ⫻ 10⫺7

PILRB

Similar to hCG2024106

5.07

2.70 ⫻ 10⫺6

IGHG3

Immunoglobulin gamma 3 heavy chain constant region

5.04

3.26 ⫻ 10⫺3

IL1R2

Interleukin 1 receptor, type II

4.82

2.69 ⫻ 10⫺4

GNA14

G alpha 14 ⫽ guanine nucleotide binding protein alpha 14

4.72

⬍1 ⫻ 10⫺7

ULK2

Unc-51-like kinase 2 (C. elegans)

4.26

1.27 ⫻ 10⫺3

BAI3

Brain-specific angiogenesis inhibitor 3

4.16

3.92 ⫻ 10⫺4

IL8

Interleukin 8

3.98

2.28 ⫻ 10⫺4

HRASLS3

HRAS-like suppressor 3

3.68

2.24 ⫻ 10⫺4

PTGS2

Prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)

3.67

1.61 ⫻ 10⫺4

PPM1A

Protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform

3.61

1.30 ⫻ 10⫺6

CYP3A7

Cytochrome P450, family 3, subfamily A, polypeptide 7

3.59

1.45 ⫻ 10⫺5

CCR1

Chemokine (C-C motif) receptor 1

3.58

1.31 ⫻ 10⫺3

CX3CR1

Chemokine (C-X3-C motif) receptor 1

3.58

1.39 ⫻ 10⫺3

UBE2K

Ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)

3.43

3.00 ⫻ 10⫺7

RPL3L

Ribosomal protein L3-like

3.42

3.00 ⫻ 10⫺7

HOXA9

Homeobox A9

3.40

2.10 ⫻ 10⫺4

TMSB10

Thymosin beta 10

3.38

1.34 ⫻ 10⫺3

ZNF644

Zinc finger protein 644

3.34

1.74 ⫻ 10⫺3

HIRA

HIR histone cell-cycle regulation defective homolog A (S cerevisiae)

3.31

2.33 ⫻ 10⫺5

LLT1

Lectin-like NK-cell receptor

3.30

4.62 ⫻ 10⫺5

PRDM15

PR domain containing 15

3.23

2.82 ⫻ 10⫺4

TIMP3

Tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinflammatory)

3.19

2.26 ⫻ 10⫺4

ACACA

Acetyl-coenzyme A carboxylase alpha

3.14

2.70 ⫻ 10⫺6

GZMA

Granzyme A (granzyme 1, cytotoxic T-lymphocyte–associated serine esterase 3)

3.12

1.19 ⫻ 10⫺3

ICOS

Inducible T-cell co-stimulator

3.09

2.63 ⫻ 10⫺4

CYP1B1

Cytochrome P450, family 1, subfamily B, polypeptide 1

3.05

3.64 ⫻ 10⫺5

LYN

Lyn ⫽ tyrosine kinase

3.02

8.78 ⫻ 10⫺5

GPA33

Glycoprotein A33 (transmembrane)

3.02

1.74 ⫻ 10⫺4

*The fold increase for each gene is the average expression in the plerixafor plus G-CSF–mobilized CD34⫹ cells from 3 rhesus divided by the average expression of the same gene in the CD34⫹ cells mobilized by plerixafor from 3 monkeys and CD34⫹ cells mobilized by G-CSF from 3 monkeys.

mobilized CD34⫹ cells are more likely to include neutrophil and macrophage precursors, and plerixafor-mobilized CD34⫹ cells are more likely to contain T-cell, B-cell, and mast cell precursors. Gene expression analysis of CD34ⴙ cells mobilized by plerixafor plus G-CSF

To assess the nature of CD34⫹ cells mobilized by the combination of plerixafor plus G-CSF, we combined the gene expression results from CD34⫹ cells mobilized with G-CSF alone and with plerixafor alone, and compared this dataset with CD34⫹ cells mobilized with plerixafor plus G-CSF. A total of 1375 genes were up-regulated in plerixafor plus G-CSF–mobilized CD34⫹ cells compared with those mobilized with G-CSF alone and plerixafor alone, and 1449 were up-regulated in CD34⫹ cells regulated with G-CSF alone and plerixafor alone (t tests, P ⬍ .05). Analysis of the pathways that contained the most genes up-regulated in CD34⫹ cells found that the genes up-regulated in the plerixafor plus G-CSF–mobilized CD34⫹ cells were more likely to belong to B-cell signaling, Fc epsilon RI signaling, Toll-like receptor, and cell cycle (Table 5). Those down-regulated in the plerixafor plus G-CSF–mobilized CD34⫹ cells were more likely to belong to ribosome, antigen processing and presentation, and vascular endothelial growth factor signaling pathways. Analysis of the specific genes up-regulated in plerixafor plus G-CSF–mobilized CD34⫹ cells found that, when compared with G-CSF alone and plerixafor alone mobilized CD34⫹ cells, plerixafor plus G-CSF–mobilized CD34⫹ cells expressed increased levels of B-cell genes: immunoglobulin alpha heavy chain constant

region, Bruton agammaglobulinemia tyrosine kinase, and immunoglobulin gamma 3 heavy chain constant region (Table 6). In addition, the T-cell gene granzyme A; the HSC gene HOXA9; an NK cell gene, lectin-like NK-cell receptor (LLT1); and a gene encoding a protein found in the extracellular matrix and macrophages tissue inhibitor of metalloproteinase (TIMP3) were upregulated (Table 6). The CD34⫹ cell genes up-regulated most by plerixafor plus G-CSF also included prostaglandin-endoperoxide synthase 2 (PTGS2) or cyclooxygenase 2 (COX2), an important enzyme in arachidonic acid metabolism and prostaglandin production. Analysis of PTGS2 and HOXA9 expression by qRT-PCR confirmed that the expression of PTGS2 was greater in plerixafor plus G-CSF–mobilized CD34⫹ cells than in those mobilized by plerixafor alone or G-CSF alone (Figure 3). The expression of HOXA9 was similar among CD34⫹ cells mobilized by each of the 3 regimens but was much greater than that for peripheral blood leukocytes (Figure 3). We also identified the genes that were down-regulated most in plerixafor plus G-CSF–mobilized CD34⫹ cells. Among the genes down-regulated most in plerixafor plus G-CSF–mobilized CD34⫹ cells were a number of ribosomal protein genes, cathepsin S (an enzyme important for antigen processing), neutrophil receptor formyl peptide receptor 1, bone morphogenic protein 2, laminin, and gamma 3 (Table 7). MIR expression analysis

A comparison of differentially expressed miR between plerixaforand G-CSF–mobilized CD34⫹ cells found that 2 miR were

From bloodjournal.hematologylibrary.org by guest on May 18, 2011. For personal use only. BLOOD, 17 SEPTEMBER 2009 䡠 VOLUME 114, NUMBER 12

PLERIXAFOR- AND G-CSF–MOBILIZED CD34⫹ CELLS

2537

Table 7. Genes whose expression was decreased the greatest in plerixafor plus G-CSF–mobilized CD34ⴙ cells compared with those mobilized by plerixafor alone and G-CSF alone Gene symbol

Description

Fold decrease*

Parametric P

CTSS

Cathepsin S

13.95

⬍ 1 ⫻ 10⫺7

C9orf3

Chromosome 9 open reading frame 3

7.64

2.16 ⫻ 10⫺4

NTRK2

Neurotrophic tyrosine kinase, receptor, type 2

5.82

⬍ 1 ⫻ 10⫺7

NMT1

N-myristoyltransferase 1

5.64

2.00 ⫻ 10⫺7

SCN1B

Sodium channel, voltage-gated, type I, beta

5.34

⬍ 1 ⫻ 10⫺7

IL16

Interleukin 16 (lymphocyte chemoattractant factor)

4.98

1.20 ⫻ 10⫺6

CYP27B1

Cytochrome P450, family 27, subfamily B, polypeptide 1

4.56

2.35 ⫻ 10⫺4

KNS2

kinesin 2 (60-70 kDa)

4.45

1.18 ⫻ 10⫺4

SMARCD2

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d,

4.373

6.81 ⫻ 10⫺4 8.89 ⫻ 10⫺5

member 2 CCL25

TECK chemokine (CCL25)

4.27

PFDN6

Prefoldin subunit 6

4.26

2.53 ⫻ 10⫺3

EIF5A

Eukaryotic translation initiation factor 5A

4.24

6.40 ⫻ 10⫺5

TYMS

Thymidylate synthetase

4.23

2.20 ⫻ 10⫺6

OCLN

Occludin ⫽ tight junction protein

4.21

2.35 ⫻ 10⫺4

NAT2

N-acetyltransferase 2 (arylamine N-acetyltransferase)

4.09

8.14 ⫻ 10⫺4

RPL23

Ribosomal protein L23

4.04

6.20 ⫻ 10⫺6

WISP2

WNT1 inducible signaling pathway protein 2

4.01

1.20 ⫻ 10⫺6

MVD

Mevalonate (diphospho) decarboxylase

3.84

2.33 ⫻ 10⫺5

BMP2

Bone morphogenetic protein 2

3.79

3.03 ⫻ 10⫺4

UXT

Ubiquitously expressed transcript

3.78

2.80 ⫻ 10⫺6

IQGAP1

IQ motif containing GTPase activating protein 1

3.67

4.00 ⫻ 10⫺6

KCNA5

Potassium voltage-gated channel, shaker-related subfamily, member 5

3.67

1.80 ⫻ 10⫺3

RPS27A

Ribosomal protein S27a ⫽ fused to ubiquitin

3.60

1.00 ⫻ 10⫺5

ECH1

Enoyl coenzyme A hydratase 1, peroxisomal

3.52

2.10 ⫻ 10⫺6

FPR1

Formyl peptide receptor 1

3.41

8.60 ⫻ 10⫺6

STAP2

Signal transducing adaptor family member 2

3.36

3.15 ⫻ 10⫺4

ZNF525

Zinc finger protein 525

3.27

8.30 ⫻ 10⫺5

LAMC3

Laminin, gamma 3

3.23

1.03 ⫻ 10⫺3

DES

Desmin

3.18

1.78 ⫻ 10⫺5

RPS15

Ribosomal protein S15

3.17

2.80 ⫻ 10⫺6

*The fold decrease for each gene is the average expression in the plerixafor plus G-CSF–mobilized CD34⫹ cells from 3 rhesus divided by the average expression of the same gene in the CD34⫹ cells mobilized by plerixafor from 3 monkeys and CD34⫹ cells mobilized by G-CSF from 3 monkeys.

up-regulated in plerixafor-mobilized CD34⫹ cells, miR-34c and miR-432 (Table 8). A miR, miR-126, that was previously found to be up-regulated in G-CSF–mobilized peripheral blood CD34⫹ cells compared with peripheral blood leukocytes19 was up-regulated in G-CSF–mobilized CD34⫹ cells compared with plerixaformobilized CD34⫹ cells (Table 8). The expression of miR-155, which is up-regulated during macrophage activation,20 was also greater in G-CSF–mobilized CD34⫹ cells. In addition, among the

miR up-regulated in G-CSF–mobilized CD34⫹ cells were miR-21, miR-129-3p, miR-146b, miR-99a, miR-126*, and miR-10a*. We also identified miRs differentially expressed between CD34⫹ cells mobilized by plerixafor plus G-CSF and those mobilized by G-CSF alone and plerixafor alone. The levels of miR-126* and miR-142-3p were greater in plerixafor plus G-CSF–mobilized CD34⫹ cells than in those mobilized by either G-CSF alone (Table 9) or plerixafor alone (Table 10). The

Table 8. miRs that were differently expressed between plerixafor-mobilized CD34ⴙ cells and G-CSF–mobilized CD34ⴙ cells Increased in plerixafor-mobilized CD34ⴙ cells miR

Increased in G-CSF–mobilized CD34ⴙ cells

Fold increase*

P

miR

hsa-miR-34c

1.69

.031

hsa-miR-21

3.67

1.72 ⫻ 10⫺4

hsa-miR-432*

1.45

.032

hsa-miR-126*

2.80

1.21 ⫻ 10⫺3

mmu-miR-129-3p

2.62

3.67 ⫻ 10⫺3

hsa-miR-146b

2.31

1.35 ⫻ 10⫺3

hsa-miR-99a

2.13

.011

hsa-miR-126*

2.08

9.23 ⫻ 10⫺3

hsa-miR-10a*

1.98

.019

hsa-miR-424

1.90

8.57 ⫻ 10⫺3

hsa-miR-126

1.70

.041

hsa-miR-155

1.65

.033

hsa-miR-335

1.55

.049

CD34⫹

Fold increase†

P

*The fold increase is the average expression of each microRNA in cells from 3 rhesus macaque given plerixafor divided by the average expression of the same microRNA in 3 monkeys given G-CSF. †The fold increase is the average expression of each microRNA in CD34⫹ cells from 3 rhesus macaque given G-CSF divided by the average expression of the same microRNA in 3 monkeys given plerixafor.

From bloodjournal.hematologylibrary.org by guest on May 18, 2011. For personal use only. 2538

BLOOD, 17 SEPTEMBER 2009 䡠 VOLUME 114, NUMBER 12

DONAHUE et al

Table 9. miRs that were differently expressed between plerixafor plus G-CSF–mobilized CD34ⴙ cells and those mobilized with G-CSF alone Increased in plerixafor ⴙ G-CSF–mobilized CD34ⴙ cells miR

Fold increase*

Increased in G-CSF–mobilized CD34ⴙ cells P

miR 10⫺3

Fold increase†

P

hsa-miR-142-3p

3.27

3.34 ⫻

sv40-miR-S1-5p

2.56

hsa-miR-142-3p

3.15

3.04 ⫻ 10⫺3

hsa-miR-335

2.17

.045

hsa-miR-142-5p

2.91

3.95 ⫻ 10⫺3

hsa-mir-615

2.00

.03

hsa-miR-29b

2.36

7.71 ⫻ 10⫺3

hsa-miR-363*

1.94

.012

hsa-miR-101

2.34

8.77 ⫻ 10⫺3

mmu-miR-10b

1.81

.012

hsa-miR-525

2.18

8.19 ⫻ 10⫺3

hsa-mir-622

2.16

.019

3.17 ⫻ 10

⫺3

mmu-mir-691

1.80

.01

hsa-miR-299-3p

1.77

.018 8.92 ⫻ 10⫺3

hsa-miR-487b

2.15

9.93 ⫻ 10⫺3

mmu-miR-346

1.72

hsa-miR-126*

2.11

9.92 ⫻ 10⫺3

mmu-mir-615

1.71

.01

hsa-miR-199b

2.10

.012

hsa-miR-320

1.61

.031

mmu-mir-804

2.09

0.016

hcmv-miR-UL70-3p

1.56

.03

hsa-miR-486

2.08

3.63 ⫻ 10⫺3

kshv-miR-K12-10a

1.54

.038

hsa-miR-197

1.99

6.74 ⫻ 10⫺3

hsa-miR-518f*

1.54

.029

hsa-miR-19a

1.98

.01

mmu-mir-423

1.51

.044

rno-miR-343

1.97

.015

hsa-mir-765

1.47

.048

hsa-miR-15a

1.97

.011

hsa-miR-19b

1.96

.012

hsa-miR-29c

1.87

.026

hsa-miR-27a

1.74

.017

hsa-miR-374

1.71

.027

hsa-miR-30e-5p

1.70

.044

hsa-miR-29a

1.68

.029

hsa-mir-662

1.66

.02

mmu-miR-489

1.63

.025

hsa-mir-421

1.60

.03

hsa-miR-20a

1.60

.025

hsa-miR-106a

1.59

.022

hsa-miR-203

1.58

.032

hsa-miR-20b

1.58

.023

ebv-mir-BART3

1.57

.022

hsa-miR-17-5p

1.55

.034

*The fold increase is the average expression of each microRNA in CD34⫹ cells from 3 rhesus macaque given plerixafor plus G-CSF divided by the average expression of the same microRNA in CD34⫹ cells from 3 monkeys given G-CSF. †The fold increase is the average expression of each microRNA CD34⫹ cells from 3 rhesus given G-CSF divided by the average expression of the same microRNA in CD34⫹ cells from 3 monkeys given plerixafor plus G-CSF.

expression of miR-21 was also increased in plerixafor plus G-CSF–mobilized CD34⫹ cells compared with plerixaformobilized CD34⫹ cells (Table 10). The expression of miR-126*, miR-155, miR-142-3p, miR-1293p, and miR-99a were also measured by qRT-RCR (Figure 4). This analysis confirmed that the expression of miR-126* and miR142-3p was greater in plerixafor plus G-CSF–mobilized CD34⫹ cells than in the other 2 types of CD34⫹ cells. In addition, the analysis confirmed that the expression of miR-99a, miR-129-3p, and miR-155 was greater in G-CSF–mobilized CD34⫹ cells than plerixafor-mobilized CD34⫹ cells.

Discussion Plerixafor has recently been approved by the US Food and Drug Administration for use with G-CSF to mobilize PBSCs for autologous transplantation. Because plerixafor is now clinically available, it will also likely be used to mobilize stem cells in the 1% to 5% of allogeneic donors who mobilize HSCs poorly in response to G-CSF.21,22 The number of CD34⫹ cells mobilized by the combination of plerixafor and G-CSF is significantly greater than either agent alone. Because the success of allogeneic transplants is highly dependent on rapid myeloid engraftment and immune reconstitution in the transplant recipient, it is important to deter-

mine whether the critical in vivo biological activity or potency of HSCs mobilized by plerixafor and plerixafor plus G-CSF is similar to those mobilized by G-CSF. We used a surrogate assay for potency testing, global gene and microRNA expression analysis, to compare CD34⫹ cells mobilized by plerixafor, G-CSF, and the combination of the 2 agents. Although many of the same genes were up-regulated in CD34⫹ cells mobilized by either plerixafor or G-CSF, the expression of a large number of genes also differed between CD34⫹ cells mobilized by these 2 agents. G-CSF–mobilized CD34⫹ cells were more likely to express neutrophil and mononuclear phagocyte genes than those mobilized by plerixafor. In addition, G-CSF–mobilized CD34⫹ cells also expressed high levels of miR-155, which is expressed in maturing dendritic cells.20 In contrast, plerixaformobilized CD34⫹ cells were more likely to express B-, T-, and mast cell genes. These results suggest that G-CSF mobilizes a greater proportion of HSCs that are committed toward myeloid differentiation, whereas those mobilized by plerixafor are more likely to be committed toward B, T, and mast cells. Surprisingly, the combination of plerixafor plus G-CSF mobilized a population of CD34⫹ cells that were distinct from CD34⫹ cells mobilized by either agent alone. CD34⫹ cells mobilized by plerixafor plus G-CSF were more likely to express B-cell and T-cell genes than G-CSF–mobilized and plerixafor-mobilized CD34⫹ cells. In addition, plerixafor plus G-CSF–mobilized CD34⫹ cells

From bloodjournal.hematologylibrary.org by guest on May 18, 2011. For personal use only. BLOOD, 17 SEPTEMBER 2009 䡠 VOLUME 114, NUMBER 12

PLERIXAFOR- AND G-CSF–MOBILIZED CD34⫹ CELLS

2539

Table 10. miRs that were differently expressed between plerixafor plus G-CSF–mobilized CD34ⴙ cells and those mobilized with plerixafor alone Increased in plerixafor ⴙ G-CSF–mobilized CD34ⴙ cells miR

Increased in plerixafor-mobilized CD34ⴙ cells

Fold increase*

P

hsa-miR-21

5.98

4.29 ⫻ 10⫺5

Fold increase†

P

hsa-mir-615

miR

2.06

.0305

hsa-miR-126*

3.91

1.6 ⫻ 10

hsa-miR-126

3.34

2.90 ⫻ 10⫺4

mmu-miR-329

2.01

.0210

sv40-miR-S1-5p

1.88

hsa-miR-142-3p

2.88

.0129

8.98 ⫻ 10⫺4

kshv-miR-K12-1

1.79

hsa-miR-142-3p

.0143

2.75

1.03 ⫻ 10⫺3

kshv-miR-K12-10a

1.78

.0103

hsa-miR-29b

2.57

2.92 ⫻ 10⫺3

mmu-mir-709

1.69

.0235

hsa-miR-142-5p

2.56

1.22 ⫻ 10⫺3

rno-miR-327

1.68

.0155

hsa-miR-101

2.54

3.01 ⫻ 10⫺3

hsa-miR-518f*

1.63

.0312

hsa-miR-29c

2.44

8.59 ⫻ 10⫺3

hsa-mir-612

1.62

.0353

hsa-miR-345

2.39

8.68 ⫻ 10⫺3

hcmv-miR-UL70-3p

1.58

.0457

mmu-mir-705

1.53

.0365

ebv-mir-BART6

1.51

.0475

hsa-mir-557

1.51

.0404

hsa-miR-363*

1.50

.0440

⫺3

hsa-miR-424

2.34

.011

hsa-miR-27a

2.21

9.84 ⫻ 10⫺3

hsa-miR-148a

2.21

.044

hsa-miR-29a

2.15

5.57 ⫻ 10⫺3

mmu-mir-804

2.09

.01

hsa-miR-146b

2.07

5.72 ⫻ 10⫺3

hsa-miR-19a

2.07

3.23 ⫻ 10⫺3

hsa-miR-24

2.03

.014

hsa-miR-19b

1.93

4.96 ⫻ 10⫺3

hsa-miR-146a

1.90

.021

hsa-miR-374

1.72

.025

hsa-miR-487b

1.71

.043

hsa-miR-486

1.71

.034

hsa-miR-15a

1.71

.029

hsa-miR-18a

1.69

.025

rno-miR-374

1.62

.03

hsa-miR-30e-5p

1.62

.04

hsa-miR-20a

1.59

.04

hsa-miR-106a

1.53

.043

*The fold increase in the average expression of each microRNA in CD34⫹ cells from 3 rhesus macaque given plerixafor plus G-CSF divided by the average expression of the same microRNA in CD34⫹ cells from 3 monkeys given plerixafor. †The fold increase in the average expression of each microRNA in CD34⫹ cells from in 3 rhesus macaque given plerixafor divided by the average expression of the same microRNA in CD34⫹ cells from monkeys given plerixafor plus G-CSF.

were also remarkable for their increased expression of miR142-3p and miR-142-5p. miR-142-3p and miR-142-5p are highly expressed in T cells,23 and miR-142-3p has been found to be increased in childhood B-cell precursor acute lymphoblastic leukemia.24 The results suggest plerixafor plus G-CSF mobilizes a greater proportion of B- and T-cell precursors than either G-CSF or plerixafor alone. It is not clear if plerixafor, G-CSF, or their combination mobilizes a more primitive HSC. CD34⫹ cells mobilized by plerixafor expressed greater levels of CXCR4 and FLT3 than G-CSF–mobilized CD34⫹ cells. Increased expression of CXCR4 in the plerixafor-mobilized population is not surprising and is consistent with the nature of the mobilization protocol, that is, interfering with the binding of CXCR4 and CXCL12. Thus, CD34⫹ cells mobilized with plerixafor alone or in combination with G-CSF should be expressing CXCR4. FLT3 is a member of the receptor tyrosine kinase family and plays an important role in hematopoietic homeostasis.25 It is expressed on both common lymphoid and multipotent progenitors. Higher FLT3 expression was observed in those CD34⫹ cells mobilized with plerixafor alone or with the combination plerixafor plus G-CSF than with G-CSF alone. These increases in expression were confirmed by qRT-PCR analysis. We also found that the expression of COX2 was up-regulated in plerixafor plus G-CSF–mobilized CD34⫹ cells. COX2 is an inducible enzyme that metabolizes arachidonic acid to a number of prostaglandins, including prostaglandin E2 (PGE2). PGE2 has been found to be important in hematopoiesis. In zebrafish PGE2

increases HSC numbers, and in mice it enhances spleen colonyforming units and the frequency of long-term repopulating cells.26 PEG2 also has been found to enhance the expression of CXCR4 on mouse CD34⫹ cells.27 This suggests that greater quantities of these progenitors are among plerixafor-mobilized and plerixafor plus G-CSF–mobilized CD34⫹ cells than G-CSF–mobilized CD34⫹ cells. G-CSF–mobilized CD34⫹ cells, however, have increased levels of miR-126, miR-126*, and miR-10a* compared with plerixaformobilized CD34⫹ cells. miR-126 and miR-10a have previously been found to be expressed by CD34⫹ cells.19 In addition to being expressed by HSCs, miR-126 is also expressed by megakaryocytes and endothelial cells and is important in angiogenesis.28,29 miR126* and miR-10a* are mirror images of miR-126 and miR-10a, respectively, and they are derived from the same pre-miR stem loop, so it is not surprising that both miR-126 and miR-126* were expressed in G-CSF–mobilized CD34⫹ cells. These 2 miR, however, target different genes, and they may play a different role in HSC function. We found that the levels of miR-126 and miR-126* were greater in plerixafor plus G-CSF–mobilized CD34⫹ cells than in the other types of CD34⫹ cells. Another miR whose expression was increased in G-CSF–mobilized CD34⫹ cells was miR-99a, which is also expressed by megakaryocytes.29 These results suggest that G-CSF–mobilized CD34⫹ cells may contain a wider range of lineage progenitors. Transplantation studies will be required to determine whether plerixafor, G-CSF, or the combination of these agents mobilizes a more primitive HSC.

From bloodjournal.hematologylibrary.org by guest on May 18, 2011. For personal use only. 2540

DONAHUE et al

BLOOD, 17 SEPTEMBER 2009 䡠 VOLUME 114, NUMBER 12

Figure 4. Analysis of differentially expressed mobilized CD34ⴙ cell miRs by qRT-PCR. The expression of miR-126*, miR-129-3p, miR-142-3p, miR-99, and miR-155 was analyzed by qRT-PCR in CD34⫹ cells from 3 rhesus macaque mobilized by plerixafor (A), G-CSF (G), or plerixafor plus G-CSF (A⫹G). The expression of the 5 microRNAs in leukocytes that were not absorbed by anti-CD34 from the 9 mobilized PBSC concentrates (CD34⫺) are shown as a control.

The finding that plerixafor, G-CSF, and plerixafor plus G-CSF mobilize different types of CD34⫹ cells is consistent with other studies that found that different agents mobilized different populations of HSCs. In a mouse model, Pitchford and colleagues have found that G-CSF mobilized greater quantities of hematopoietic progenitor cells than plerixafor but plerixafor mobilized greater quantities of endothelial progenitor cells than G-CSF.30 They also found that the combination of G-CSF plus plerixafor mobilized greater quantities of hematopoietic progenitors than either agent alone, but not endothelial progenitors. The results of this study have several implications for clinical HSC transplantation with mobilized PBSCs. Because plerixafor, G-CSF, and plerixafor plus G-CSF mobilize different subpopulations of CD34⫹ cells, the potency of PBSCs mobilized by these agents may differ. The rate of engraftment and profile of transplantassociated comorbidities may differ between recipients of allogeneic transplants with plerixafor-mobilized PBSCs and those who receive G-CSF–mobilized PBSCs. In addition, the dose of CD34⫹ cells required for successful engraftment may be different for plerixafor- and plerixafor plus G-CSF–mobilized PBSC concentrates. A preliminary study of allogeneic transplantations involving 20 HLA-compatible siblings has shown that plerixafor-mobilized

PBSCs resulted in prompt neutrophil and platelet engraftment although a relatively low median dose of CD34⫹ cells was given, 2.9 ⫻ 106 per kilogram of recipient weight.4 This same study found that plerixafor-mobilized PBSCs contained greater quantities of CD3⫹, CD4⫹, and CD8⫹ cells than G-CSF–mobilized PBSCs, but the rates of acute and chronic graft versus host disease that were similar to those of historic controls who received G-CSF– mobilized PBSCs at the same institution.4 Our data suggest that if similar quantities of CD34⫹ cell are administered, those mobilized with G-CSF may result in faster neutrophil recovery and those mobilized with plerixafor or plerixafor plus G-CSF may have more rapid B-cell and T-cell recovery. Larger controlled studies will be required to assess similarities and differences in rates of engraftment, acute and chronic graft-versus-host disease, and immune reconstitution between plerixafor- and G-CSF–mobilized PBSCs. Clinical correlates will be required to determine the minimum and optimal CD34⫹ cell doses of plerixafor-mobilized and plerixafor plus G-CSF–mobilized PBSCs required for successful transplantations and to determine whether the rate and sequence of immune recovery differs among the mobilization regimens. In conclusion, the composition of CD34⫹ cells in PBSC concentrates is dependent on the mobilization regimen, and the

From bloodjournal.hematologylibrary.org by guest on May 18, 2011. For personal use only. BLOOD, 17 SEPTEMBER 2009 䡠 VOLUME 114, NUMBER 12

composition of CD34⫹ cells mobilized by a combination of agents is not a simple mixture of the cells mobilized by single agents. Unique expression profile signatures appear between populations. G-CSF, plerixafor, and plerixafor plus G-CSF mobilized different populations of CD34⫹ cells, but further in vitro and clinical studies are needed to compare the potency of those cells mobilized by these agents.

Acknowledgments This study was funded by the Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, and the Department of Transfusion Medicine, Clinical Center, National Institutes of Health.

PLERIXAFOR- AND G-CSF–MOBILIZED CD34⫹ CELLS

2541

Authorship Contribution: R.E.D. designed the study, analyzed data, and wrote the manuscript; P.J. helped design the study, performed studies, analyzed data, and wrote the manuscript; A.C.B., M.E.M., J.R., and E.W. performed studies and analyzed data; and D.F.S. helped design the study, analyzed data, and wrote the manuscript. Conflict-of-interest disclosure: A drug used in this study, plerixafor, was provided by Genzyme. The authors declare no competing financial interests. Correspondence: David Stroncek, MD, Department of Transfusion Medicine, Clinical Center, National Institutes of Health, 10 Center Dr, Bldg 10, Rm 1C711, Bethesda, MD 20892-1184; e-mail: [email protected].

References 1. Burroughs L, Mielcarek M, Little MT, et al. Durable engraftment of AMD3100-mobilized autologous and allogeneic peripheral-blood mononuclear cells in a canine transplantation model. Blood. 2005;106(12):4002-4008. 2. Larochelle A, Krouse A, Metzger M, et al. AMD3100 mobilizes hematopoietic stem cells with long-term repopulating capacity in nonhuman primates. Blood. 2006;107(9):3772-3778. 3. Flomenberg N, Devine SM, Dipersio JF, et al. The use of AMD3100 plus G-CSF for autologous hematopoietic progenitor cell mobilization is superior to G-CSF alone. Blood. 2005;106(5):18671874. 4. Devine SM, Vij R, Rettig M, et al. Rapid mobilization of functional donor hematopoietic cells without G-CSF using AMD3100, an antagonist of the CXCR4/SDF-1 interaction. Blood. 2008;112(4): 990-998. 5. Devine SM, Flomenberg N, Vesole DH, et al. Rapid mobilization of CD34⫹ cells following administration of the CXCR4 antagonist AMD3100 to patients with multiple myeloma and nonHodgkin’s lymphoma. J Clin Oncol. 2004;22(6): 1095-1102. 6. Broxmeyer HE, Orschell CM, Clapp DW, et al. Rapid mobilization of murine and human hematopoietic stem and progenitor cells with AMD3100, a CXCR4 antagonist. J Exp Med. 2005;201(8): 1307-1318. 7. Liles WC, Broxmeyer HE, Rodger E, et al. Mobilization of hematopoietic progenitor cells in healthy volunteers by AMD3100, a CXCR4 antagonist. Blood. 2003;102(8):2728-2730. 8. Deichmann M, Kronenwett R, Haas R. Expression of the human immunodeficiency virus type-1 coreceptors CXCR-4 (fusin, LESTR) and CKR-5 in CD34⫹ hematopoietic progenitor cells. Blood. 1997;89(10):3522-3528. 9. Peled A, Petit I, Kollet O, et al. Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4. Science. 1999;283(5403):845-848. 10. Ponomaryov T, Peled A, Petit I, et al. Induction of the chemokine stromal-derived factor-1 following

DNA damage improves human stem cell function. J Clin Invest. 2000;106(11):1331-1339. 11. Jung Y, Wang J, Schneider A, et al. Regulation of SDF-1 (CXCL12) production by osteoblasts: a possible mechanism for stem cell homing. Bone. 2006;38(4):497-508. 12. Nervi B, Link DC, Dipersio JF. Cytokines and hematopoietic stem cell mobilization. J Cell Biochem. 2006;99(3):690-705. 13. Hess DA, Bonde J, Craft TP, et al. Human progenitor cells rapidly mobilized by AMD3100 repopulate NOD/SCID mice with increased frequency in comparison to cells from the same donor mobilized by granulocyte colony stimulating factor. Biol Blood Marrow Transplant. 2007; 13(4):398-411. 14. Fruehauf S, Seeger T, Maier P, et al. The CXCR4 antagonist AMD3100 releases a subset of G-CSF-primed peripheral blood progenitor cells with specific gene expression characteristics. Exp Hematol. 2006;34(8):1052-1059. 15. Liles WC, Rodger E, Broxmeyer HE, et al. Augmented mobilization and collection of CD34⫹ hematopoietic cells from normal human volunteers stimulated with granulocyte-colony-stimulating factor by single-dose administration of AMD3100, a CXCR4 antagonist. Transfusion. 2005;45(3): 295-300. 16. Donahue RE, Kirby MR, Metzger ME, Agricola BA, Sellers SE, Cullis HM. Peripheral blood CD34⫹ cells differ from bone marrow CD34⫹ cells in Thy-1 expression and cell cycle status in nonhuman primates mobilized or not mobilized with granulocyte colony-stimulating factor and/or stem cell factor. Blood. 1996;87(4):1644-1653. 17. Eisen MB, Spellman PT, Brown PO, Botstein D. Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci U S A. 1998;95(25):14863-14868. 18. Dennis G Jr, Sherman BT, Hosack DA, et al. DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol. 2003; 4(5):3. 19. Jin P, Wang E, Ren J, et al. Differentiation of two types of mobilized peripheral blood stem cells by

20.

21.

22. 23.

24.

25.

26.

27.

28.

29.

30.

microRNA and cDNA expression analysis. J Transl Med. 2008 July 22;6(1):39. Ceppi M, Pereira PM, Dunand-Sauthier I, et al. MicroRNA-155 modulates the interleukin-1 signaling pathway in activated human monocytederived dendritic cells. Proc Natl Acad Sci U S A. 2009;106(8):2735-2740. Vasu S, Leitman SF, Tisdale JF, et al. Donor demographic and laboratory predictors of allogeneic peripheral blood stem cell mobilization in an ethnically diverse population. Blood. 2008;112(5): 2092-2100. Takeyama K, Ohto H. PBSC mobilization. Transfus Apher Sci. 2004;31(3):233-243. Wu H, Neilson JR, Kumar P, et al. miRNA profiling of naive, effector and memory CD8 T cells. PLoS ONE. 2007;2(10):e1020. Ju X, Li D, Shi Q, Hou H, Sun N, Shen B. Differential microRNA expression in childhood B-cell precursor acute lymphoblastic leukemia. Pediatr Hematol Oncol. 2009;26(1):1-10. Kikushige Y, Yoshimoto G, Miyamoto T, et al. Human Flt3 is expressed at the hematopoietic stem cell and the granulocyte/macrophage progenitor stages to maintain cell survival. J Immunol. 2008; 180(11):7358-7367. North TE, Goessling W, Walkley CR, et al. Prostaglandin E2 regulates vertebrate haematopoietic stem cell homeostasis. Nature. 2007;447(7147): 1007-1111. Goichberg P, Kalinkovich A, Borodovsky N, et al. cAMP-induced PKCzeta activation increases functional CXCR4 expression on human CD34⫹ hematopoietic progenitors. Blood. 2006;107(3): 870-879. Fish JE, Srivastava D. MicroRNAs: opening a new vein in angiogenesis research. Sci Signal. 2009;2(52):e1. Garzon R, Pichiorri F, Palumbo T, et al. MicroRNA fingerprints during human megakaryocytopoiesis. Proc Natl Acad Sci U S A. 2006;103(13):50785083. Pitchford SC, Furze RC, Jones CP, Wengner AM, Rankin SM. Differential mobilization of subsets of progenitor cells from the bone marrow. Cell Stem Cell. 2009;4(1):62-72.