in the form of meningitis or tuberculoma is recognized clini- cally in 16-30% patients with miliary tuberculosis2-4 although meningeal involvement can be found ...
Feb 17, 2012 - Some of the advantages of the zebrafish animal model system, ... The zebrafish system possesses many advantages for vascular studies.
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endothelial cells (ECs) and supporting mural cells or pericytes (MCs) that are recruited from surrounding healthy tissue to form new vessels in the growing tumor.
Feb 17, 2012 - The critical cellular targets of these therapies are vascular endothelial cells (ECs) and supporting mural cells or pericytes (MCs) that are ...
Sleeping Beautyâ used in mouse for insertional somatic mutagenesis â has been adopted in zebrafish and successfully used to identify con- served and novel ...
Mar 16, 2006 - *Correspondence to: Zhiyuan Gong; Department of Biological Sciences; S3-Level 5,. 14 Science Drive 4; National University of Singapore; ...
Dec 4, 2017 - ish is emerging as a model system for myeloid malignancies. In this review, we discuss ... the brain) and other tissue-resident macrophages (17â19). It will ... advantages of the zebrafish model to study the genetic basis of malignanc
A popular method of behavioral analysis in zebrafish research is the novel ..... LPS exposure may possibly serve as a model for sickness behavior in zebrafish.
Modeling metabolic adjustment in Mycobacterium tuberculosis upon treatment with isoniazid. Authors; Authors and affiliations. Ashwini G. Bhat; Rohit Vashisht ...
Please consult your local healthcare professional for further information. Your local meningitis foundation: The Meningi
Aug 28, 2017 - help to develop patient-tailored therapies in the future. (Left panel) Patient-derived xenograft approach: cancer cells prepared from resected or ...
Dec 20, 2012 - Funding: The meningitis surveillance was supported by Sanofi Pasteur, Hib Initiative, PneumoADIP and the French Ministry of Foreign Affairs.
What is meningitis? Meningitis is inflammation of the meninges, the membranes that cover the brain and spinal cord. Ther
In this paper, we have used statistical measures and spatial deviational ellipse to determine the spatial pattern of tuberculosis within a Chennai ward population ...
Botswana College of Agriculture, P. Bag 0027, Gaborone, Botswana ... burden, but research has shown that in high disease ...... (Bhunu et al., 2008; LaSalle,.
activates ovarian cancer G-protein-coupled receptor 1 and GPR4 homologs of zebra fish. Biochemical and Biophysical Research. Communications 457:4 ...
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Li-Fraumeni syndrome (LFS) is an autosomal dominant, highly penetrant cancer predisposition that presents with a wide variety of tumor types at an early age, ...
Peter C. Hart. 2 ..... molecular basis of development and function of sensory hair cells (Ernest et al 2000). ... mutations in deafness genes (Ernest et al 2000).
and Doctorate degrees in science and engineer- ing at substantially lower rates ... ernment places on science, technology, and math education is ... 5Department of Biology, University of Minnesota, Duluth, Minnesota. 6Department of Biology ...
Meningitis is defined as inflammation of the membranes that surround the ... The
etiology of bacterial meningitis is affected most by the age of the patient.
com tinta da Índia e cultura em ágar Sabouraud a 37°C. Entre as amostras estudadas, 5 (9,25%) foram positivas. Palavras-chaves: Cryptococcus neoformans.
78 Abstracts ingediend voor het Amsterdam Kindersymposium 2013. Modeling tuberculosis meningitis in the zebrafish-. Mycobacterium marinum infection ...
Modeling tuberculosis meningitis in the zebrafishMycobacterium marinum infection model L.M. van Leeuwen (1,2), M. van der Kuip (1), W. Bitter (2), A.M. van der Sar (2), A.M. van Furth (1) (1) VU University medical center, Dept. of Pediatric Infectious Diseases, Immunology and Rheumatology, Amsterdam, The Netherlands (2) VU University medical center, Dept. Medical Microbiology and Infection control, Amsterdam, The Netherlands
INTRODUCTION Tuberculosis meningitis (TBM) is a severe extra-pulmonary manifestation of tuberculosis (TB). It occurs especially in early childhood, has a high morbidity and mortality and is often only considered when irreversible neurological damage has occurred. Characteristic pathological features of TBM are Rich foci, brain-specific granuloma formed after hematogenous spread of pulmonary TB. Little is known about the role of these foci during TBM, our aim is to unravel the pathogenesis. METHODS
To study pathogenesis we use the Danio rerio – Mycobacterium marinum infection model. Zebrafish naturally develop granuloma after infection with M. marinum, the closest genetic relative to M. tuberculosis. Embryos were infected via injection with fluorescent M. marinum in the caudal vein, hindbrain ventricle or parenchyma. Infection was analyzed 5 days later. After fixation, embryos were stained with anti-acetylated tubulin, labeling axons or anti-L-plastin, labeling phagocytes. Granuloma localization and composition was analyzed with confocal microscopy.
RESULTS
We show that after infection via different routes, clustering of bacteria and phagocytes occurs in the brain parenchyma. Infection via the bloodstream leads to granuloma formation in the brain in 50-80% of the cases. All embryos develop granuloma in the brain after direct injection of bacteria into the parenchyma. After infection via the ventricle, clusters are present in the meninges in every case and bacteria migrate to the parenchyma in 60% of the embryos.
CONCLUSION
The Zebrafish – Mycobacterium marinum infection model is a useful model to study granuloma formation in the brain. Different inoculation routes can be used for different research questions. Bacterial and host features will be further investigated in the future.
78 Abstracts ingediend voor het Amsterdam Kindersymposium 2013
