Modelling Zika Virus Infection of the Developing Human Brain ... - JoVE

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Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using. Stem Cell Derived Cerebral Organoids. Max R Salick*1, Michael F Wells*2,3, Kevin ...
Journal of Visualized Experiments

www.jove.com

Materials List for:

Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids 1

2,3

2,3

1

Max R Salick* , Michael F Wells* , Kevin Eggan , Ajamete Kaykas 1

Department of Neuroscience, Novartis Institutes for BioMedical Research

2

Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard

3

Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University

*

These authors contributed equally

Correspondence to: Ajamete Kaykas at [email protected] URL: https://www.jove.com/video/56404 DOI: doi:10.3791/56404

Materials Name

Company

Catalog Number

Enzyme-Free Detachment Reagent, ReLeSR

StemCell Technologies

5873

Dimethyl Sulfoxide (DMSO)

Sigma-Aldrich

D2650-100ML

Glutamate-supplemented DMEM/ F12 (1X)

Gibco

10565-018

N-2 Supplement (100X)

Gibco

17502-048

MEM Non-Essential Amino Acids (MEM-NEAA)

Gibco

11140-050

Earle's Balanced Salt Solution (EBSS)

GE Healthcare Life Sciences

SH30029.02

Stericup Filter (500mL)

Millipore

220009-140

Comments

(+)2.438 g/L sodium bicarbonate, (+)sodium pyruvate

Costar Ultra-Low Attachment Plate, Corning 96-Well, Round Bottom

7007

Low Residual Volume Reagent Reservoir (25mL)

Integra

4312

Pipet-Lite Multi Pipette L12-200XLS+

Rainin

17013810

Large Centrifuge, Culture PlateCompatible

Thermo Scientific

75007210

Culture Plate Centrifuge Rotor

Thermo Scientific

75005706

Vero Cells

ATCC

CCL-81

For virus expansion, if necessary

Zika Virus (Uganda Strain)

ATCC

VR-1838

Other strains may be used

Zika Virus (Puerto Rico Strain)

ATCC

VR-1843

Other strains may be used

phospho-vimentin

MBL

D076-3

mouse polyclonal, 1:250 dilution

TBR2

Abcam

ab23345

rabbit polyclonal, 1:300 dilution

MAP2

Novus

NB300-213

chicken polyclonal, 1:1500 dilution

GFAP

Cell Signaling Technologies

CST12389S

rabbit polyclonal, 1:200 dilution

D1-4G2 flavivirus envelope protein

EMD Millipore

MAB10216

mouse monoclonal, 1:500 dilution

cleaved caspase-3

Cell Signaling Technologies

9661

rabbit polyclonal, 1:200 dilution

488 anti-mouse IgG

Thermo Fisher

A-11001

goat polyclonal, 1:1000

594 anti-rabbit IgG

Thermo Fisher

A-11037

goat polyclonal, 1:1000 dilution

647 anti-chicken IgY

Thermo Fisher

A-21449

goat polyclonal, 1:1000 dilution

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