Journal of Visualized Experiments
www.jove.com
Materials List for:
Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids 1
2,3
2,3
1
Max R Salick* , Michael F Wells* , Kevin Eggan , Ajamete Kaykas 1
Department of Neuroscience, Novartis Institutes for BioMedical Research
2
Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard
3
Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University
*
These authors contributed equally
Correspondence to: Ajamete Kaykas at
[email protected] URL: https://www.jove.com/video/56404 DOI: doi:10.3791/56404
Materials Name
Company
Catalog Number
Enzyme-Free Detachment Reagent, ReLeSR
StemCell Technologies
5873
Dimethyl Sulfoxide (DMSO)
Sigma-Aldrich
D2650-100ML
Glutamate-supplemented DMEM/ F12 (1X)
Gibco
10565-018
N-2 Supplement (100X)
Gibco
17502-048
MEM Non-Essential Amino Acids (MEM-NEAA)
Gibco
11140-050
Earle's Balanced Salt Solution (EBSS)
GE Healthcare Life Sciences
SH30029.02
Stericup Filter (500mL)
Millipore
220009-140
Comments
(+)2.438 g/L sodium bicarbonate, (+)sodium pyruvate
Costar Ultra-Low Attachment Plate, Corning 96-Well, Round Bottom
7007
Low Residual Volume Reagent Reservoir (25mL)
Integra
4312
Pipet-Lite Multi Pipette L12-200XLS+
Rainin
17013810
Large Centrifuge, Culture PlateCompatible
Thermo Scientific
75007210
Culture Plate Centrifuge Rotor
Thermo Scientific
75005706
Vero Cells
ATCC
CCL-81
For virus expansion, if necessary
Zika Virus (Uganda Strain)
ATCC
VR-1838
Other strains may be used
Zika Virus (Puerto Rico Strain)
ATCC
VR-1843
Other strains may be used
phospho-vimentin
MBL
D076-3
mouse polyclonal, 1:250 dilution
TBR2
Abcam
ab23345
rabbit polyclonal, 1:300 dilution
MAP2
Novus
NB300-213
chicken polyclonal, 1:1500 dilution
GFAP
Cell Signaling Technologies
CST12389S
rabbit polyclonal, 1:200 dilution
D1-4G2 flavivirus envelope protein
EMD Millipore
MAB10216
mouse monoclonal, 1:500 dilution
cleaved caspase-3
Cell Signaling Technologies
9661
rabbit polyclonal, 1:200 dilution
488 anti-mouse IgG
Thermo Fisher
A-11001
goat polyclonal, 1:1000
594 anti-rabbit IgG
Thermo Fisher
A-11037
goat polyclonal, 1:1000 dilution
647 anti-chicken IgY
Thermo Fisher
A-21449
goat polyclonal, 1:1000 dilution
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