silybin, silydianin and silychristine, obtained from the fruit and seeds of the Silybum marianum (milk thistle).1 It is widely prescribed by herbalists and has almost ...
research
Modulation of cutaneous wound healing by silymarin in rats l Objective: To investigate the effect of topical application of silymarin on full-thickness cutaneous wounds in rats. l Method: A full-thickness cutaneous defect (2×2cm) was induced on the back of 85 male and female Wister rats. The animals were randomly divided into four groups (n=20 in each group), treated with 1ml basal cream (placebo group), low-dose (6mg/ml/rat) and high-dose (12mg/ml/rat) silymarin, and untreated (control). Five rats remained uninjured to serve as comparisons for biomechanical analysis. Wounds were evaluated 10, 20 and 30 days after injury, through histopathologic, biochemical and biomechanical analyses. l Results: There was a significant (p 75% fibrocytes
> 75% of collagen fibres are dense and have large size
1 — Highly mature > 50% fibrocytes
> 50% of collagen fibres are dense and are of large size
2 — Moderately mature > 25% fibrocytes
> 25% of collagen fibres are dense and are medium sized
3 — immature > 75% fibroblasts
Collagen fibres are not dense, but are medium sized
4 — Highly immature
Collagen fibres are not dense and are of small size
Inflammatory cells predominant
were adjusted to pH 7 and subjected to chloramineT oxidation. The absorbance of the coloured adduct formed with the aldehyde–perchloric acid reagent at 60°C was recorded at 550nm, after cooling for 5min. The data were then multiplied by 7.46 to convert them from hydroxyproline content to collagen content.29
j o u r n a l o f wo u n d c a r e v o l 2 1 , n o 9 , S e p t e m b e r 2 0 1 2
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After thawing, the samples pieces were dried in a hot-air oven at 60–70°C until a constant weight was achieved. They were then hydrolysed with 6N HCl for 2 hours at 120°C. The hydrolysed samples
Score
459
research between groups. The base scoring data were statistically analysed using the Kruskal-Wallis non-parametric test and Mann-Whitney U tests were performed to test for significant differences between the groups. All statistical analyses were performed using SPSS software (v19.0; SPSS Inc.). Differences of p
