Modulation of cutaneous wound healing by silymarin in rats

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silybin, silydianin and silychristine, obtained from the fruit and seeds of the Silybum marianum (milk thistle).1 It is widely prescribed by herbalists and has almost ...
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Modulation of cutaneous wound healing by silymarin in rats l Objective: To investigate the effect of topical application of silymarin on full-thickness cutaneous wounds in rats. l Method: A full-thickness cutaneous defect (2×2cm) was induced on the back of 85 male and female Wister rats. The animals were randomly divided into four groups (n=20 in each group), treated with 1ml basal cream (placebo group), low-dose (6mg/ml/rat) and high-dose (12mg/ml/rat) silymarin, and untreated (control). Five rats remained uninjured to serve as comparisons for biomechanical analysis. Wounds were evaluated 10, 20 and 30 days after injury, through histopathologic, biochemical and biomechanical analyses. l Results: There was a significant (p  75% fibrocytes

> 75% of collagen fibres are dense and have large size

1 — Highly mature > 50% fibrocytes

> 50% of collagen fibres are dense and are of large size

2 — Moderately mature > 25% fibrocytes

> 25% of collagen fibres are dense and are medium sized

3 — immature > 75% fibroblasts

Collagen fibres are not dense, but are medium sized

4 — Highly immature

Collagen fibres are not dense and are of small size

Inflammatory cells predominant

were adjusted to pH 7 and subjected to chloramineT oxidation. The absorbance of the coloured adduct formed with the aldehyde–perchloric acid reagent at 60°C was recorded at 550nm, after cooling for 5min. The data were then multiplied by 7.46 to convert them from hydroxyproline content to collagen content.29

j o u r n a l o f wo u n d c a r e v o l 2 1 , n o 9 , S e p t e m b e r 2 0 1 2

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After thawing, the samples pieces were dried in a hot-air oven at 60–70°C until a constant weight was achieved. They were then hydrolysed with 6N HCl for 2 hours at 120°C. The hydrolysed samples

Score

459

research between groups. The base scoring data were statistically analysed using the Kruskal-Wallis non-parametric test and Mann-Whitney U tests were performed to test for significant differences between the groups. All statistical analyses were performed using SPSS software (v19.0; SPSS Inc.). Differences of p