ICRISAT in short. International Crops Research Institute for the Semi-Arid Tropics
. 5 mandate crops: Chickpea, pigeonpea, groundnut. Pearl millet, sorghum.
Molecular breeding & genetic engineering for drought tolerance What scope ? Vincent Vadez ICRISAT
ICRISAT in short International Crops Research Institute for the Semi-Arid Tropics
5 mandate crops: Chickpea, pigeonpea, groundnut Pearl millet, sorghum 1.3 Billions people in drought-prone areas (India/Africa) Drought is a major research focus for ICRISAT
Groundnut - Transpiration efficiency DREB1A Wild type JL24
RD19 RD12 RD20 RD2 RD11
Dendrogram based on soil moisture value where Tr declines and number of days to deplete soil water
Groundnut - Transpiration efficiency DREB1A TE under well watered and drought stress in 5 DREB1A events and JL24
JL 24 RD 19 RD 12 RD 20 RD 2 RD 11
TE - WW
TE - DS
2.05 4.31 5.13 3.19 4.09 4.96
4.29 4.99 4.63 4.52 6.12 5.59
Most DREB1A have larger TE than JL24 under WW Two events with higher TE under both water regimes
Groundnut - Transpiration efficiency
TE (g DW/kg water)
DREB1ARange of values in TE in a RIL population and in DREB1A groundnut 7 6 5 4 3 2 1 0
Min Max
RIL
DREB Well Watered
DREB drought
Larger range of variation for TE in DREB1A
Conclusion MAS (pearl millet and sorghum) Need to saturate QTL areas to increase the efficiency Pyramid with other QTLs Get rid of linkge drag Root-Chickpea: More markers, QTL identification, Start marker-assisted breeding TE-Groundnut: More markers Re-synthesize the cultigen from its ancestors Transgenic approach New traits