Supplementary materials
Article title: Molecular phylogeny of Shortia sensu lato (Diapensiaceae) based on multiple nuclear sequences
Journal name: Plant Systematics and Evolution
Authors: Hiroyuki Higashi1*, Hajime Ikeda2, Hiroaki Setoguchi1. 1
Graduate School of Human and Environmental Studies, Kyoto University, Yoshida-nihonmatsu-cho, Sakyo-ku, Kyoto 606-8501,
Japan; 2 Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki City, Okayama 710-0046, Japan *Corresponding author: Hiroyuki Higashi
E-mail:
[email protected]
Fig. S1
Phylogenetic relationships of Diapensiaceae based on each locus. Likelihood bootstrap values (>50%) are above nodes
Fig. S2
Ancestral distribution reconstruction using S-DIVA. Star indicates the vicariant event that occurred at the nodes at a high
probability
Fig. S3
a Schizocodon soldanelloides and b Shortia uniflora. In a, upper and lower figures show flowering individuals and raceme
inflorescence, respectively
Table S1 Sample information and DDBJ/EMBL/GenBank accession numbers of nucleotide sequences Species and individual number*
Locality**
Voucher number
28
147
A5
B3
B7
C1
C23
C26
Shortia uniflora 1 Shortia uniflora 2 Shortia rotundifolia 1 Shortia rotundifolia 2 Shortia rotundifolia 3 (S . exappendiculata ) Shortia sinensis 1 Shortia sinensis 2 Shortia galacifolia Schizocodon soldanelloides 1 Schizocodon soldanelloides 2 Schizocodon ilicifolius 1 Schizocodon ilicifolius 2 Schizocodon ilicifolius var. intercedens 1 Schizocodon ilicifolius var. intercedens 2 Diapensia lapponica 1 Diapensia lapponica 2 Berneuxia thibetica Galax urceolata
Kyoto, Japan Kyoto, Japan Iriomote island, Japan Iriomote island, Japan Taiwan Yunnan, China Yunnan, China specimen (North America) Daisen, Japan Kitadake, Japan Kinpusan, Japan Kinpusan, Japan Kofu, Japan Kofu, Japan Furano, Japan Kisokoma, Japan Yunnan, China Cultivated (North America)
H. Setoguchi JP2005(KYO) H. Setoguchi JP2005(KYO) H. Setoguchi JP2445 (KYO) H. Setoguchi JP2445 (KYO) H. Setoguchi 04T-M2912 (HAST) H. Setoguchi 2004370 (KYO, KUN) H. Setoguchi 2004370 (KYO, KUN) R. T. Clausen 5646 (A) H. Higashi 24 (KYO) no voucher no voucher no voucher H. Setoguchi S2003(KYO) H. Setoguchi S2003(KYO) no voucher no voucher Peng, Setoguchi, Momohara & Yukawa 206 (TNS, KUN) no voucher
AB897961 AB897962 AB897956 AB897957 AB897958 AB897959 AB897960 AB897965 AB897951 AB897948 AB897952 AB897953 AB897949 AB897950 AB897954 AB897955 AB897963 AB897964
AB897978 AB897979 AB897973 AB897974 AB897975 AB897976 AB897977 AB897982 AB897971 AB897966 AB897967 AB897968 AB897969 AB897970 AB897980 AB897981 AB897972 -
AB897895 AB897899 AB897897 AB897894 AB897903 AB897898 AB897902 AB897911 AB897906 AB897905 AB897904 AB897907 AB897908 AB897909 AB897896 AB897900 AB897901 AB897910
AB897923 AB897924 AB897925 AB897926 AB897927 AB897921 AB897922 AB897929 AB897915 AB897912 AB897916 AB897917 AB897913 AB897914 AB897919 AB897920 AB897918 AB897928
AB897991 AB897992 AB897988 AB897989 AB897990 AB897985 AB897984 AB897987 AB897993 AB897994 AB897995 AB897986 AB897983 AB897996 AB897997
AB898011 AB898010 AB898007 AB898008 AB898009 AB898005 AB898006 AB898004 AB898003 AB898000 AB898001 AB897998 AB897999 AB898002 AB898012 -
AB897933 AB897934 AB897930 AB897931 AB897932 AB897935 AB897936 AB897947 AB897941 AB897940 AB897938 AB897939 AB897942 AB897943 AB897944 AB897945 AB897937 AB897946
AB898026 AB898027 AB898023 AB898024 AB898025 AB898018 AB898019 AB898017 AB898016 AB898020 AB898021 AB898015 AB898022 AB898013 AB898014 AB898028
*The genus Schizocodon is incorporated with Shortia sensu lato . **The region in parentheses indicates the distribution of the species.
Table S2 Primers used in this study Marker
Sequences
F/R
Reference
28
AAGCTCTGACAAGATCTCATC CCAGAGACACAGTTCTTG GGATATATCAAAACGAAACCAC GTCACTAAAATTGTAACTAATC ATATGTTCCCTATGGTAACTGG TCATTGATAAACAATTTGTTGGAC CTGATAACAGTGAGGATC TCCATGCTGGAATGAGAG GATGAACCTTTGGCTCTGGA CACGAACGAGGAACATGGTA ATGGGCTTCATTCAGTCCAAC TTGTGAGGAGAACCTCAGC TCTGCTGATCCTGAGGCTTT TGGGCGTGGACAAGATTG GAGAGCGGTCCTGAAGTTTG GACAGCCAATGCTGTGCT
Forward Reverse Forward Reverse Forward Reverse Forward Reverse Forward Reverse Forward Reverse Forward Reverse Forward Reverse
Developed for this study Developed for this study Developed for this study Developed for this study Developed for this study Developed for this study Developed for this study Developed for this study Wei et al. (2005) Wei et al. (2005) Developed for this study Developed for this study Wei et al. (2005) Developed for this study Wei et al. (2005) Wei et al. (2005)
147 A5 B3 B7 C1 C23 C26
