Monitoring the Effect of Different Storage Conditions of Cold-Pressed ...
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Monitoring the Effect of Different Storage Conditions of Cold-Pressed ...
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International Journal of Chemical Engineering and Applications, Vol. 7, No. 4, August 2016
Monitoring the Effect of Different Storage Conditions of Cold-Pressed Rice Bran Oil Chutima Ponchurdchai and Jirada Singkhonrat
The composition of γ-oryzanol is mixture of phytosteryl ferulates including 4-hydroxy-3-methoxycinnamic acid (ferulate) esters of cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, β-sitosterol ferulate, and campesterol ferulate that can be separated and quantified by high performance liquid chromatography [6], [7]. In addition, the determination of the proportion of fatty acid in oils and fats can be analyzed by proton nuclear magnetic resonance spectroscopy, 1H NMR spectroscopy in a short period of time and also has a great deal of structural information, required small amount of sample. There are many reports of NMR application for olive oil, walnut oil and other vegetable oils by several authors [8]-[10]. The gas chromatography-mass spectrometry, GC-MS analysis and the integration of signals in the 1H NMR spectra were used for determining the fatty acid compositions and molecular degradation during a year storage. The antioxidant contents and its activity of RBO were also investigated. The aim of this study was to determine the impact of storage temperature on changes in molecular degradation for shelf life of RBOs and to evaluate the stability by monitoring acid, iodine and peroxide values from different storage conditions.
Abstract—This study investigated the effect of different storage conditions of cold-pressed rice bran oil from 2 different regions in Thailand (Lopburi province, LRBO and Yasothorn province, YRBO). All samples were stored at different temperature as opened and sealed bottle for 12 months. Fatty acid composition by nuclear magnetic resonance, NMR spectroscopy, chemical properties such as acid value, iodine value and peroxide value were reported. Also, the antioxidant content and its activity were evaluated, including FRAP assay (Ferric reducing antioxidant potential) and DPPH radical scavenging assay (1, 1-diphenyl-2-picrylhydrazyl radical reducing power methods). Cold-pressed rice bran oils sealed bottles for 12 months and freshly opened bottles have shown no affect to acid value, iodine value and peroxide value (p
