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Cell Reports, Volume 22
Supplemental Information
AP2s Mutations Impair Calcium-Sensing Receptor Trafficking and Signaling, and Show an Endosomal Pathway to Spatially Direct G-Protein Selectivity Caroline M. Gorvin, Angela Rogers, Benoit Hastoy, Andrei I. Tarasov, Morten Frost, Silvia Sposini, Asuka Inoue, Michael P. Whyte, Patrik Rorsman, Aylin C. Hanyaloglu, Gerda E. Breitwieser, and Rajesh V. Thakker
SUPPLEMENTAL INFORMATION AP2σ mutations impair calcium-sensing receptor trafficking and signaling, and reveal an endosomal pathway that spatially-directs G-protein selectivity Caroline M Gorvin1, Angela Rogers1, Benoit Hastoy2, Andrei I Tarasov2, Morten Frost1, Silvia Sposini3, Asuka Inoue4,5, Michael P Whyte6, Patrik Rorsman2, Aylin C Hanyaloglu3, Gerda E Breitwieser7, Rajesh V Thakker1
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Supplemental Figures FIGURE S1
Development of HEK293 AP2σ Stable Cell-lines, Related to Figure 1
Stable overexpression of AP2σ protein (wild-type (WT, R15) or mutant (C15, H15, L15)) in HEK293 cells compared to parental native (NA) cells was confirmed by (A) Western blot analysis and (B) densitometric analysis from 4 independent blots. Data are shown as mean+SEM. **p
