Am J Physiol Heart Circ Physiol 289: H1326 –H1334, 2005. First published April 29, 2005; doi:10.1152/ajpheart.00323.2005.
Myogenic contraction in rat skeletal muscle arterioles: smooth muscle membrane potential and Ca2⫹ signaling Neela Kotecha1,† and Michael A. Hill1,2 1
Microvascular Biology Group, School of Medical Sciences, RMIT University, Bundoora, Victoria; and 2Department of Physiology and Pharmacology, University of New South Wales, Kensington, New South Wales, Australia Submitted 31 March 2005; accepted in final form 27 April 2005
Kotecha, Neela, and Michael A. Hill. Myogenic contraction in rat skeletal muscle arterioles: smooth muscle membrane potential and Ca2⫹ signaling. Am J Physiol Heart Circ Physiol 289: H1326 –H1334, 2005. First published April 29, 2005; doi:10.1152/ajpheart.00323.2005.—The present studies examined relationships between intraluminal pressure, membrane potential (Em), and myogenic tone in skeletal muscle arterioles. Using pharmacological interventions targeting Ca2⫹ entry/ release mechanisms, these studies also determined the role of Ca2⫹ pathways and Em in determining steady-state myogenic constriction. Studies were conducted in isolated and cannulated arterioles under zero flow. Increasing intraluminal pressure (0 –150 mmHg) resulted in progressive membrane depolarization (⫺55.3 ⫾ 4.1 to ⫺29.4 ⫾ 0.7 mV) that exhibited a sigmoidal relationship between extent of myogenic constriction and Em. Thus, despite further depolarization, at pressures ⬎70 mmHg, little additional vasoconstriction occurred. This was not due to an inability of voltage-operated Ca2⫹ channels to be activated as KCl (75 mM) evoked depolarization and vasoconstriction at 120 mmHg. Nifedipine (1 M) and cyclopiazonic acid (30 M) significantly attenuated established myogenic tone, whereas inhibition of inositol 1,4,5-trisphosphate-mediated Ca2⫹ release/entry by 2-aminoethoxydiphenylborate (50 M) had little effect. Combinations of the Ca2⫹ entry blockers with the sarcoplasmic reticulum (SR) inhibitor caused a total loss of tone, suggesting that while depolarization-mediated Ca2⫹ entry makes a significant contribution to myogenic tone, an interaction between Ca2⫹ entry and SR Ca2⫹ release is necessary for maintenance of myogenic constriction. In contrast, none of the agents, in combination or alone, altered Em, demonstrating the downstream role of Ca2⫹ mobilization relative to changes in Em. Large-conductance Ca2⫹-activated K⫹ channels modulated Em to exert a small effect on myogenic tone, and consistent with this, skeletal muscle arterioles appeared to show an inherently steep relationship between Em and extent of myogenic tone. Collectively, skeletal muscle arterioles exhibit complex relationships between Em, Ca2⫹ availability, and myogenic constriction that impact on the tissue’s physiological function. voltage-operated calcium channels; large-conductance calcium-activated potassium channels
typically show an inherent ability to contract to an increase in intraluminal pressure. The developed active force is considered myogenic, occurring independently of neurohumoral influences (5, 18). Although the exact mechanism linking increased intraluminal pressure and contraction remain unclear, it is generally accepted that an increase in intraluminal pressure produces depolarization of smooth muscle cell membranes (7, 17) with subsequent depolarization-induced Ca2⫹
ARTERIOLES
† Deceased February 2005. Address for reprint requests and other correspondence: M. A. Hill, Dept. of Physiology and Pharmacology, School of Medical Sciences, Univ. of New South Wales, High St., Kensington, New South Wales 2052, Australia (e-mail:
[email protected]). H1326
entry being necessary for myogenic contraction (5). However, although Ca2⫹ influx across the plasma membrane is essential for the myogenic response, there are disparate reports regarding whether voltage-gated Ca2⫹ entry is the prime determinant of myogenic tone (8, 10, 13, 14, 21). Supporting the involvement of alternate mechanisms, studies of single smooth muscle cells undergoing controlled stretch, as a model of myogenic activation, have shown that this mode of mechanical stimulation leads to Ca2⫹ entry, which is only partially blocked by nifedipine (6). Furthermore, evidence has been presented for the involvement of mechanisms utilizing signaling pathways involving protein kinase C and Rho kinase (21). A further complicating factor is that heterogeneity in the mechanisms underlying myogenic reactivity may exist between tissues. Thus the contribution of non-voltage-dependent mechanisms to myogenic contraction remains uncertain (12). In addition to Ca2⫹ entry, intracellular Ca2⫹ dynamics play a pivotal role in the electromechanical coupling in vascular smooth muscle cells of the arterial wall (16, 17, 20, 23, 28). Thus Ca2⫹ release from the sarcoplasmic reticulum (SR) and localized events including Ca2⫹ sparks, which may affect the activity of plasma membrane large-conductance Ca2⫹-activated K⫹ (BKCa) channels, have been implicated in the expression of myogenic reactivity (16, 17, 20). Although this is an attractive hypothesis, the exact links between these events and mechanically induced events such as changes in membrane potential (Em) and ion channel activation remain unresolved. To date, much of our knowledge of the contribution of changes in Em to myogenic contraction has been derived from studies of cerebral vessels (7, 17, 20, 28). The general applicability of these observations has not been established, and given observed heterogeneity with respect to the ability of various tissues to demonstrate autoregulatory responses (19), it may be reasonable to expect variation in the relationships between Em and levels of myogenic tone. Additionally, the degree of negative feedback by mechanisms such as BKCa channels may vary between tissues. Thus such channels may act more to limit myogenic vasoconstriction in cerebral vessels compared with those in resting skeletal muscle (15). The present studies therefore aimed to define the 1) relationships between intraluminal pressure, Em, and extent of myogenic tone for skeletal muscle arterioles and 2) implications of these relationships on physiological function. Using pharmacological interventions targeting Ca2⫹ entry/release mechanisms, these studies also sought to determine the role of these Ca2⫹ pathways and Em in determining steady-state myogenic constriction. The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
0363-6135/05 $8.00 Copyright © 2005 the American Physiological Society
http://www.ajpheart.org
MEMBRANE POTENTIAL AND CA2⫹ PATHWAYS IN MYOGENIC TONE MATERIALS AND METHODS
Animals Male Sprague-Dawley rats (200 –300 g body wt; n ⫽ 76) were used. Animals were housed in a facility equipped with 12:12-h light-dark cycle and free access to rat chow and drinking water. The Animal Ethics and Experimentation Committee of RMIT University approved all procedures. Isolated Arteriole Preparation Rats were anesthetized with thiopental sodium (100 mg/kg) given intraperitoneally. Cremaster muscles were removed, and segments of first-order arteriole were isolated as previously described (25). Arteriole segments were cannulated onto glass micropipettes, secured using 10-0 monofilament suture, and mounted in a 3-ml tissue chamber. The vessel preparation was positioned on the stage of an inverted microscope and continually superfused (2– 4 ml/min) with physiological salt solution (PSS; in mM: 111 NaCl, 25.7 NaHCO3, 4.9 KCl, 2.5 CaCl2, 1.2 MgSO4, 1.2 KH2PO4, 11.5 glucose, and 10 HEPES). Arterioles were gradually pressurized, under zero flow, to 70 mmHg by connecting the inflow pipette to a height-adjustable fluid reservoir and warmed to 34°C during a 60-min equilibration period and allowed to develop spontaneous tone. Vessel length was adjusted before development of tone by increasing segment length such that pressure steps to 120 mmHg did not cause lateral bowing of the vessel. This procedure has been verified to result in optimal myogenic and agonist responsiveness. Lumen diameter (as an index of mechanical response) was monitored using video microscopy in conjunction with an imagebased tracking program (Diamtrak, Adelaide, Australia) (27), and the data were stored using a MacLab analog-to-digital system. Em Measurements Intracellular recordings of Em, using glass microelectrodes filled with 2 M KCl (tip resistances of 100 –200 M⍀) and an Axoclamp 2B amplifier (Axon Instruments), were made in pressurized arteriole preparations. Impalements were made using a Leitz precision micromanipulator (Leica Microsystems, Victoria, Australia) within the field of microscope view and in a region of the vessel demonstrating typical vasoreactivity. Drugs and Chemicals Nifedipine (Sigma) was prepared daily as a stock solution (10 mM) in DMSO. Cyclopiazonic acid (CPA; Sigma) was dissolved (10 mM) in DMSO and stored at ⫺20°C. 2-Aminoethoxydiphenylborate (2APB; Aldrich) was prepared as a 100-mM stock solution in ethanol. Iberiotoxin (100 nM, Sigma) was dissolved in 0.95 saline and stored at ⫺20°C. Phenylephrine (10 mM), ACh (10 mM), and adenosine (100 mM) were dissolved in water. All subsequent dilutions were made in PSS as required, and 75 mM KCl (75KPSS) was made by raising the extracellular [K⫹] of the PSS to 75 mM with direct replacement of NaCl with KCl.
H1327
mmHg). Additional studies were performed to determine the contribution of BKCa channels to the level of pressure-induced membrane depolarization and myogenic tone. After taking baseline measurements, vessels were treated with iberiotoxin (100 nM, 15 min), and measurements were subsequently repeated. Effect of nifedipine, CPA, and 2-APB on vascular tone and Em at 70 mmHg. The endothelial layer was removed from all vessels by intraluminal passage of air bubbles followed by PSS to remove cellular debris. Endothelial removal was demonstrated functionally by a lack of vasodilatation to ACh (10 M), while dilator responses to adenosine (0.3 mM) were maintained. When steady-state diameter had been attained, smooth muscle cells were impaled to determine Em. To determine the contribution of selected Ca2⫹ release/entry pathways, arterioles were exposed to either CPA (30 M, an inhibitor of SR Ca-ATPase), 2-APB [50 M, inhibitor of inositol 1,4,5-trisphosphate (IP3)-mediated Ca2⫹ influx and release], or nifedipine (1 M, inhibitor of Ca2⫹ influx through VOCC) for at least 20 min before repeating measurements of diameter and Em. Additional experiments were performed to assess the effects of a combination of nifedipine and 2-APB. Furthermore, the effects of inhibiting a single Ca2⫹ entry pathway in combination with the Ca2⫹ release pathway were studied (nifedipine ⫹ CPA or 2-APB ⫹ CPA). In all experiments, Em and diameter measures were performed when steady-state diameter was reached. Control experiments were conducted to verify the efficacy of nifedipine and CPA. In one series, arterioles were exposed to 75KPSS to obtain a control KCl-induced constriction, which was then compared with that after 20-min exposure to 1 M nifedipine. In separate experiments, arterioles were exposed to 0 mM Ca2⫹ PSS containing 2 mM EGTA for 1 min before measuring a control constriction (an indicator of intracellular Ca2⫹ release) to phenylephrine (10 M). The arteriole was then superfused for 30 min in Ca2⫹-containing PSS and a further 10 min in PSS containing 30 M CPA to deplete the intracellular Ca2⫹ stores. The arteriole was then exposed to 0 mM Ca2⫹ PSS, as above, for 1 min before repeating the response to phenylephrine. Comparative effect of nifedipine on the development and maintenance of myogenic tone. As differences have been suggested in the events underlying the development and maintenance of myogenic tone (29), the effectiveness of nifedipine was compared when applied at 0 or 70 mmHg. Studies were conducted using paired vessel segments, and responses were determined as changes in vessel diameter. After basal myogenic tone was established at 70 mmHg, intraluminal pressure was decreased to 0 mmHg for 60 min to negate all pressure-induced signals. One group of vessels was treated with nifedipine (1 M) for 20 min before increasing the intraluminal pressure to 70 mmHg. The second set of vessels was exposed to vehicle for 20 min before being returned to 70 mmHg, and nifedipine (1 M) was added subsequently. Measurement of passive arteriolar diameter. At the completion of each protocol, arterioles were made passive by superfusion (10 min) with 0 mM Ca2⫹ PSS containing 2 mM EGTA. Passive diameters were then measured at appropriate intraluminal pressures.
Protocols Effect of intraluminal pressure and vasoactive agents on myogenic tone and Em. After initial equilibration at 70 mmHg, intraluminal pressure was varied between 0 and 150 mmHg. At each chosen pressure, the arteriole was allowed to equilibrate for 5–10 min to reach steady-state diameter before determining the corresponding Em. Additionally, the steady-state effect of a hyperpolarizing stimulus, i.e., ACh, was determined at 50 and 120 mmHg. KCl, an agent that primarily relies on Ca2⫹ entry through voltageoperated Ca2⫹ channels (VOCC) to cause constriction, was used to assess whether a limit on VOCC-mediated vascular contractility occurs at higher intraluminal pressures. 75KPSS-induced constriction and depolarization were compared at two pressures (50 and 120 AJP-Heart Circ Physiol • VOL
Analysis and Statistics Data are expressed as means ⫾ SE. Simple comparisons of means were performed using Student’s t-test. Multiple comparisons were determined using ANOVA with the paired least-squares difference post hoc test. Values of P ⬍ 0.05 were considered significant. Sigmoidal curves were generated using Graphpad Prism software. Myogenic tone refers to active vessel diameter expressed as percentage of passive diameter at 70 mmHg unless stated otherwise. The extent of myogenic contraction (active myogenic tone) occurring in response to changes in intraluminal pressure was obtained by subtracting the diameter of the active vessel at a particular pressure from its passive diameter in 0 mM Ca2⫹ PSS and expressed in micrometers.
289 • OCTOBER 2005 •
www.ajpheart.org
H1328
MEMBRANE POTENTIAL AND CA2⫹ PATHWAYS IN MYOGENIC TONE RESULTS
Increasing intraluminal pressures over the range 0 to 150 mmHg were associated with progressive membrane depolarization from ⫺55.3 ⫾ 4.1 to ⫺29.4 ⫾ 0.7 mV (Fig. 1A). Plotting active myogenic tone against Em yielded a steep sigmoidal relationship (Fig. 1B). Implications of Em-Myogenic Tone Relationship
Fig. 1. Relationships between intraluminal pressure, membrane potential (Em), and diameter in cannulated skeletal muscle arterioles. A: increasing intraluminal pressure evokes active constriction of rat skeletal muscle arterioles together with membrane depolarization. Diameters are normalized to passive diameter at 70 mmHg (%d70 passive; 138 ⫾ 5 m; n ⫽ 8). B: relationship between Em and active myogenic tone (passive ⫺ active diameter) as calculated from data shown in A. Numbers in parentheses indicate intraluminal pressures in mmHg. This relationship between Em and active myogenic tone can be used to estimate effectiveness, in terms of diameter response, of hyperpolarizing stimuli. Dashed lines indicate that a 10-mV change in Em at lower pressures is associated with a significantly more pronounced change in diameter compared with the same change in Em at higher pressures (see text). For A and B, results are shown as means ⫾ SE.
From the data in Fig. 1B it can be seen that a 10-mV change in Em from ⫺30 to ⫺40 mV is associated with arteriolar relaxation of ⬇20 m, whereas the same magnitude of membrane hyperpolarization (from ⫺40 mV to ⫺50 mV) leads to a more pronounced relaxation of ⬇70 m. This was experimentally supported by examining arteriole responsiveness to ACh at intraluminal pressures of 50 and 120 mmHg. The amplitude of hyperpolarization and the corresponding changes in diameter in response to ACh (1 M; n ⫽ 5) at these intraluminal pressures are shown in Fig. 2A. While there is no significant difference in the amplitude of hyperpolarization at the two pressures, there is a significant difference in the resulting change in diameter. This supports the proposition that the functional effect of a change in Em, at the two different pressures, is governed by the sigmoidal relationship between Em and myogenic tone. To assess whether there was a limit on VOCC-mediated contractility at higher intraluminal pressures, 75KPSS-induced constriction was compared at pressures of 50 and 120 mmHg and therefore different levels of myogenic tone. Figure 2, B and C, shows that while there were significant differences between the amplitude (⌬) of KCl-induced depolarization (29.2 ⫾ 1.3 mV at 50 mmHg compared with 21.5 ⫾ 1.5 mV at 120 mmHg; P ⫽ 0.002; n ⫽ 6) and constriction (47.8 ⫾ 4.5 vs. 31.4 ⫾ 3.8 m; P ⫽ 0.02) at the two pressures, there were no differences in either the final (or absolute) lumen diameter (19.6 ⫾ 1.1 vs.
Fig. 2. Effect of intraluminal pressure on responsiveness to vasoactive agents. A: Em and diameter changes (⌬) in response to ACh (1 M) at differing levels of myogenic tone (n ⫽ 5). A low and high level of myogenic tone was induced by intraluminal pressures of 50 and 120 mmHg, respectively. B and C: Em and diameter changes, respectively, in response to 75 mM KCl physiological salt solution (75KPSS) at intraluminal pressures of 50 and 120 mmHg. Results are shown as means ⫾ SE. *P ⬍ 0.05.
AJP-Heart Circ Physiol • VOL
289 • OCTOBER 2005 •
www.ajpheart.org
MEMBRANE POTENTIAL AND CA2⫹ PATHWAYS IN MYOGENIC TONE
H1329
Effect of Nifedipine, CPA, and 2-APB on Em and Myogenic Tone at 70 mmHg
Fig. 3. A: simultaneous recording of Em and diameter in an arteriole displaying vasomotion (at 0.11 Hz) at 70 mmHg. In this example, peak spike depolarization preceded peak constriction by 2.04 ⫾ 0.26 s, calculated over 7 spikes. B: simultaneous recording of Em and diameter during ACh exposure for an arteriole at 50 mmHg. Hyperpolarization in response to 1 M ACh preceded relaxation response by 0.75 s. C and D: sections of the above tracings using an expanded scale to highlight that Em changes precede diameter changes.
22.8 ⫾ 1.6 m) or Em (⫺9.8 ⫾ 0.3 vs. ⫺10.7 ⫾ 0.3 mV). Hence, the difference in amplitude of constriction would appear to reflect differences in the level of myogenic tone as, importantly, arterioles remained responsive to KCl at the higher pressure. The relationship between Em and mechanical response was observed in arterioles that displayed spontaneous vasomotion and by application of ACh, a hyperpolarizing stimulus. Vasomotion was observed in ⬃5% of the isolated, pressurized arterioles that were studied. Simultaneous continuous measurements of diameter and Em were recorded in five such experiments. Peak spike depolarization preceded peak arteriole constriction by 2.05 ⫾ 0.51 s (n ⫽ 5; for each n value, an average was determined for 5–10 spikes). An example tracing is shown in Fig. 3A with an expanded timescale tracing in Fig. 3C. In response to ACh, hyperpolarization preceded relaxation; an example tracing is shown in Fig. 3B together with an expanded timescale in Fig. 3D.
All experiments examining Ca2⫹ sources were conducted in deendothelialized arterioles (70 mmHg) with myogenic tone of 50.3 ⫾ 1.4% (n ⫽ 58) and Em of ⫺39.04 ⫾ 1.02 mV (n ⫽ 25). Inhibition of L-type Ca2⫹ channels with nifedipine (1 M) resulted in a significant but incomplete loss of myogenic tone (68 ⫾ 2.1% vs. control of 51.9 ⫾ 2.2%; n ⫽ 11; Fig. 5). In contrast, there was no significant change in the associated Em (⫺37.2 ⫾ 1.6 vs. control of ⫺37.0 ⫾ 0.8 mV; n ⫽ 10). Nifedipine (1 M) was shown to be effective by its ability to inhibit 75KPSS-induced constriction (10.9 ⫾ 2.0 m vs. control of 73.5 ⫾ 7.7 m, P ⫽ 0.006; n ⫽ 4), suggesting that this concentration was functionally effective in blocking voltagegated Ca2⫹ entry. The SR Ca2⫹ ATPase inhibitor CPA (30 M) had a biphasic effect on arteriole diameter at 70 mmHg (Fig. 5). After an initial constriction, there was a significant subsequent loss of tone (66.7 ⫾ 3.3%) relative to pre-CPA treatment diameter (50.0 ⫾ 3.2%; n ⫽ 6). After 20 min exposure to CPA, Em remained unchanged (⫺40.8 ⫾ 2.4 vs. control of ⫺40.6 ⫾ 2.1 mV; n ⫽ 6) despite loss of tone. The efficacy of CPA to deplete the stores was verified by its ability to attenuate the contraction to phenylephrine (10 M) in 0 mM Ca2⫹ superfusate. Phenylephrine-induced constrictions (54.2 ⫾ 13.7 m) were significantly attenuated in the presence of 30 M CPA (1.8 ⫾ 1.8 m; P ⫽ 0.02; n ⫽ 5). Unlike nifedipine and CPA, 2-APB (50 M) did not have any significant effect on either steady-state diameter (43.1 ⫾ 4% vs. control of 44.9 ⫾ 4.2%; n ⫽ 9) (Fig. 5) or Em (⫺35.3 ⫾ 1.7 mV vs. control of ⫺36.3 ⫾ 1.9 mV; n ⫽ 9). Combining any of the two pharmacological interventions of Ca2⫹ entry/ release pathways resulted in almost complete loss of myogenic tone (Fig. 6) again without a significant change in Em. These observations were not affected by the order in which the various inhibitors were added. Addition of CPA to arterioles
Contribution of BKCa Channels to Arteriolar Smooth Muscle Em To determine the contribution of BKCa channels, pressure-Em relationships were compared in the absence and presence of iberiotoxin (100 nM). At pressures of 30, 50, 70, and 120 mmHg, iberiotoxin caused significant vasoconstriction and depolarization. For example, at 70 mmHg, iberiotoxin caused a depolarization of 6.9 mV with a corresponding vasoconstriction of 15% (Fig. 4). AJP-Heart Circ Physiol • VOL
Fig. 4. Effect of large-conductance Ca2⫹-activated K⫹ channel (BKCa) inhibition on arteriolar diameter (A) and Em (B). At intraluminal pressures of 30, 50, 70, and 120 mmHg, iberiotoxin (IBTx; 100 nM) caused vasoconstriction (A) and depolarization (B). Results are shown as means ⫾ SE; n ⫽ 6 –9. *P ⬍ 0.05.
289 • OCTOBER 2005 •
www.ajpheart.org
H1330
MEMBRANE POTENTIAL AND CA2⫹ PATHWAYS IN MYOGENIC TONE
Fig. 5. Left: representative traces for the effects of nifedipine (Nif; A), cyclopiazonic acid (CPA; B), and 2-aminoethoxydiphenylborate (2-APB; C) on vessel diameter. Dashed lines represent passive diameter in 0 mM Ca2⫹ PSS. Right: histograms show group diameter data expressed as percentage of passive diameter at 70 mmHg. All experiments shown were conducted on arterioles, without endothelium, at an intraluminal pressure of 70 mmHg. Values are shown as means ⫾ SE. Em values associated with drug treatments were not significantly different from control values (refer to text).
tween Em and Ca2⫹ mobilization were examined. Voltagedependent and -independent mechanisms of Ca2⫹ mobilization were both shown to contribute to myogenic constriction while not having a measurable effect on pressure-induced changes in steady-state Em. The data, however, are further complicated by the observation that different phases of the myogenic reactivity (i.e., development and maintenance; 29) appear to utilize different Ca2⫹ entry/release mechanisms. Consistent with data obtained in other tissues (7, 20, 32), increases in intraluminal pressure were found to result in vascular smooth muscle depolarization. Derivation of the relationship between the extent of myogenic constriction and Em revealed a steep sigmoidal relationship over the pressure range of 0 –150 mmHg. Importantly, and of potential physiological relevance, it was noted that for pressures above 70 mmHg, there was little further constriction despite continued membrane depolarization. This could not be simply explained by a mechanical limitation on constriction as the vessels maintained at 120 mmHg remained responsive to 75KPSS. As the constrictor effects of K⫹ are predominately mediated via the activation of L-type VOCC and subsequent Ca2⫹ entry, this further suggests that these channels were capable of activation both at a pressure of 120 mmHg and at the corresponding Em of the arteriole. Although the underlying reason for the plateau in the myogenic constriction-Em relationship (Fig. 1B) is uncertain, it has important implications in that hyperpolarizing stimuli may have limited capacity to elicit dilation at higher intraluminal pressures. Support for a limitation to endothelium-dependent
that had previously been exposed to 2-APB and nifedipine had no further effect on myogenic tone (data not shown). Comparative Effect of Nifedipine on the Development and Maintenance of Myogenic Tone Figure 7 illustrates the comparative effect of nifedipine on the development and maintenance of myogenic tone. When added to arterioles in which myogenic tone had been abolished by lowering intraluminal pressure to 0 mmHg, nifedipine totally prevented the development of tone on returning arterioles to 70 mmHg (Fig. 7A). In contrast, in arterioles where tone was allowed to redevelop (by returning to 70 mmHg), subsequent addition of nifedipine only partially dilated vessels toward passive (Fig. 7B). The differential effect of the same concentration of nifedipine suggests a difference in the relative contribution of dihydropyridine-sensitive VOCCs to the developmental and maintenance phases of myogenic constriction. DISCUSSION
The present study examined relationships between Em and levels of myogenic tone in isolated, cannulated skeletal muscle arterioles held under zero-flow conditions. The data demonstrate a complex relationship between Em and extent of myogenic contraction that is physiologically relevant, for example in regard to the setting of vascular tone and the efficacy of hyperpolarization-mediated vasorelaxants. The latter may be particularly important in states of increased peripheral resistance that would be expected to be associated with enhanced smooth muscle membrane depolarization relative to that seen under normotensive conditions. In addition, relationships beAJP-Heart Circ Physiol • VOL
Fig. 6. Left: representative traces illustrating the effect of combinations of agents manipulating Ca2⫹ mobilization on arteriolar diameter. A: CPA ⫹ nifedipine. B: CPA ⫹ 2-APB. C: 2-APB ⫹ nifedipine. Experiments were performed at 70 mmHg. Dashed line represents passive diameter in 0 mM Ca2⫹ PSS. Right: histograms show group diameter data expressed as percentage of passive diameter at 70 mmHg. All experiments shown were conducted on arterioles, without endothelium, at an intraluminal pressure of 70 mmHg. Values are shown as means ⫾ SE. Em values associated with drug treatments were not significantly different from control values. *P ⬍ 0.05.
289 • OCTOBER 2005 •
www.ajpheart.org
MEMBRANE POTENTIAL AND CA2⫹ PATHWAYS IN MYOGENIC TONE
Fig. 7. Effect of nifedipine on the development and maintenance of myogenic tone in paired arterioles (n ⫽ 5 for each protocol). After initial development of myogenic tone (at 70 mmHg), intraluminal pressure was reduced to 0 mmHg to negate all pressure-induced signals. Nifedipine (nifed; 1 M) or vehicle was added 20 min before a step increase in pressure (0 –70 mmHg). Diameter measurements are not shown for the period of 0 mmHg intraluminal pressure. A: when nifedipine was added before the 0- to 70-mmHg pressure step, vessels failed to regain significant myogenic tone. B: in the absence of nifedipine, vessels responded to the 0- to 70-mmHg pressure step with development of a level of tone that was not significantly different from the initial tone. Despite subsequent addition of nifedipine, and relaxation, arterioles retained a significant level of myogenic tone. Results are presented as means ⫾ SE; P ⬍ 0.05.
dilation at higher pressures was demonstrated in the present study by comparing the effectiveness of ACh at pressures of 50 and 120 mmHg. Although extensive studies were not performed to dissect the various components of the ACh-mediated dilation, we have previously shown that there is significant involvement of an endothelium-dependent hyperpolarizing component (26). Nevertheless, as the nonhyperpolarizing components of the ACh response were not inhibited in the present studies, the effect of pressure-induced changes in Em on the hyperpolarizing effect of ACh may be underestimated. As a result of these effects, the relationship between myogenic tone and Em should be taken into account when considering the effectiveness of vasoactive stimuli that exert their actions through modulation of arteriolar smooth muscle Em. The results of the present studies, while supporting previous data from Knot et al. (20) obtained from cerebral arterioles, also suggest that differences may exist between vascular beds. Comparing the current data to that of Knot et al. (20) shows an upward shift (toward more depolarized values for a given intraluminal pressure) in the pressure-Em relationship for the skeletal muscle arterioles (Fig. 8A). Interestingly, the largest AJP-Heart Circ Physiol • VOL
H1331
differences between the data sets are evident at pressures below ⬇80 mmHg, which correspond to the region of most rapid change in the Em-myogenic tone relationship (Fig. 8). Given evidence for heterogeneity in the distribution of ion channels in vascular tissues, a possible explanation could relate to underlying differences in reliance on a hyperpolarizing influence such as has been suggested for the BKCa channel. Studies using the BKCa inhibitor iberiotoxin did demonstrate a contribution of these channels to both Em and vessel diameters over the pressure range 30 –120 mmHg. Over this pressure range, intracellular Ca2⫹ is known to increase (37) and progressive depolarization occurs favoring activation of BKCa channels. However, compared with the work of Brayden and Nelson (3), it appears that the contribution of the BKCa channel is greater in cerebral vessels compared with cremaster muscle arterioles. Thus, in the study of Brayden and Nelson (3), inhibition of BKCa channels caused a vasoconstriction of 41 ⫾ 9% (at 60 mmHg), whereas in the present study, a vasoconstriction of only 15 ⫾ 3% was observed (at 70 mmHg). However, in terms of change in Em, there was a similar change of 7 ⫾ 1 mV in cerebral vessels (3) and 6.9 ⫾ 1.3 mV in the present study. It is worthwhile noting that a 15% constriction is predictably associated with a change of 7 mV (at 70 mmHg) in the skeletal muscle arterioles (Fig. 1B). In contrast to the cerebral vessel studies referred to above, Jackson and Blair (15) have suggested that in small myogenically active skeletal muscle arterioles, BKCa channels are “silent” under resting in vivo conditions but can be recruited
Fig. 8. Comparison of data from cerebral vessels (■) [data replotted from Knot et al. (20)] with skeletal muscle arterioles (Œ) from the present study shows an upward shift in the pressure-Em relationship for skeletal muscle arterioles with largest differences between data sets evident at pressures below ⬇80 mmHg (A). Above this pressure, cerebral vessels (⬇200 m) exhibit a lesser extent of myogenic constriction compared with skeletal muscle arterioles (⬇150 m) (B). Note that for both vessel sets, active tone is plotted relative to the passive diameter at each pressure. Data have been normalized as cerebral vessels are slightly larger than cremaster muscle vessels. Numbers in parentheses indicate intraluminal pressures (in mmHg).
289 • OCTOBER 2005 •
www.ajpheart.org
H1332
MEMBRANE POTENTIAL AND CA2⫹ PATHWAYS IN MYOGENIC TONE
during agonist-induced vasoconstriction. This may reflect heterogeneity in KCa channel subtypes with a possible underlying difference in Ca2⫹ set point (15). Alternatively, while estimates of global cytoplasmic Ca2⫹ appear similar in the two tissues, interactions between the cell membrane and the superficial SR controlling focal Ca2⫹ release may differ for the vessel types. If such heterogeneity exists, it remains uncertain as to whether this reflects differences in tissue distribution (i.e., skeletal muscle vs. cerebral) or vessel size. At a minimum, comparison at the molecular level is required to establish whether regional differences exist with respect to BKCa (subtype) distribution. At a physiological level, such differences may importantly reflect adaptive or protective mechanisms such that relative to resting skeletal muscle arterioles, cerebral vessels are able to maintain a more dilated state (Fig. 8B). It is also conceivable that the apparent difference in the relationship between diameter and smooth muscle membrane potential for cerebral and skeletal muscle arterioles is unrelated to differences in the activity, and/or expression, of BKCa channels. Preliminary analysis of the iberiotoxin experiments of the present study suggests that the data points approximate a similar relationship to that for control conditions as shown in Fig. 8B. Further studies are, however, required to obtain data in the presence of BKCa inhibition over the entire range of pressures presented in Figs. 1 and 8. Regardless of this, it appears that resting Em, per se, cannot be used to predict myogenic tone when comparing between vessels from different vascular beds. While this study did not find an exclusive role for voltagegated Ca2⫹ entry (see below) in myogenic constriction, evidence was provided for a temporal relationship between Em and mechanical activity. In particular, this was shown for hyperpolarizing stimuli and in continuous records of vessels exhibiting spontaneous vasomotion (Fig. 3). With regard to the latter, spikes in Em were observed, on average, to occur 2 s before peak vasoconstriction. In separate experiments, it was found that peak changes in intracellular [Ca2⫹] precede constriction by ⬇1.5 s (data not shown). Collectively, these data suggest a sequence of events whereby in skeletal muscle arterioles, vasomotion is triggered by rhythmic changes in Em that promote Ca2⫹ entry and subsequent constriction (1, 9). Interestingly, in earlier in vivo studies of small arterioles (diameter ⬃20 m), it was shown that while inhibition of voltage-dependent Ca2⫹ entry did not abolish myogenic tone, it effectively blocked vasomotion (10). These results are consistent with the notion that voltage-gated Ca2⫹ entry may not be the only source of activator Ca2⫹ contributing to the myogenic response. Sources of Ca2⫹ Underlying Myogenic Constriction In the presence of VOCC blockade by nifedipine, there was a significant, but incomplete, loss of myogenic tone. Thus while a significant influx of Ca2⫹ appeared to be mediated by nifedipine-sensitive channels, the data differ from studies suggesting that depolarization-linked Ca2⫹ entry is solely responsible for the generation of active myogenic tone (5). In contrast, data consistent with the present results are provided by studies demonstrating that ⬃50% of Ca2⫹ influx into isolated smooth muscle cells subjected to controlled stretch was not AJP-Heart Circ Physiol • VOL
prevented by nifedipine (6). In addition, as mentioned above, we have previously shown (10) that nifedipine did not abolish myogenic tone in third-order arterioles (diameter ⬃20 m) although rhythmic vasomotion was markedly inhibited. Collectively, it therefore appears that Ca2⫹ required for myogenic tone can be supplied via both VOCC- and non-VOCC-dependent sources. Exposure of arterioles to CPA to deplete SR Ca2⫹ stores resulted in a significant loss of myogenic tone, suggesting a role for Ca2⫹ stores in the generation and/or maintenance of myogenic constriction. These data are consistent with several studies examining the effect of SR Ca2⫹ ATPase inhibitors on pressure- and stretch-induced vascular tone (20, 35). The present study further suggests that both Ca2⫹ entry and SR Ca2⫹ release are required as the combination of CPA and nifedipine effected an almost complete loss of tone. The combination of inhibitors did not, however, prevent pressureinduced depolarization, consistent with both of these Ca2⫹ mobilizing events being distal to membrane depolarization in the overall signaling pathway. As an additional approach for studying the role of Ca2⫹ entry and Ca2⫹ release, studies were performed using the putative IP3 receptor blocker 2-APB. This agent has been previously reported to inhibit store depletion-mediated Ca2⫹ entry (22, 34), and such pathways are active in pressurized arterioles (30). Caution must, however, be taken in interpretation of such data as 2-APB has also been suggested to inhibit store depletion-mediated Ca2⫹ entry by more direct actions (31); nevertheless, the agent inhibits this axis of Ca2⫹ entry and was considered suitable for the goals of this study. 2-APB alone showed little effect on myogenic tone under steady-state conditions (70 mmHg), indicating that Ca2⫹ entry mechanisms inhibited by this compound were not essential. Similarly to the combination of CPA and nifedipine described above, 2-APB and nifedipine resulted in a near total loss of myogenic tone. Furthermore, treatment of arterioles with 2-APB and CPA led to a nearly complete loss of myogenic tone. While the presence of 2-APB by itself did not compromise myogenic tone, there was complete loss of myogenic tone in the presence of 2-APB and CPA. It is not clear why all tone was lost under these conditions, as Ca2⫹ influx through VOCCs would be expected to be maintained as these agents do not alter pressure-induced changes in Em. Furthermore, 2-APB does not inhibit depolarization-mediated constriction in the vessels studied (30). With regard to this, Wesselman et al. (36) have demonstrated that in rat mesenteric resistance vessels, sensitization to VOCC-mediated Ca2⫹ influx is necessary to affect myogenic constriction. While it is plausible that 2-APB and CPA somehow interfere with Ca2⫹ sensitization mechanisms, this was beyond the scope of the current study. Collectively these data are, however, consistent with a dynamic relationship between Ca2⫹ entry and SR Ca2⫹ release being necessary for the maintenance of myogenic constriction, as has been proposed by Nelson and colleagues (17, 20, 28). Disparate results regarding the role of voltage-dependent Ca2⫹ entry in myogenic phenomena may arise from several factors. The strength of the myogenic response varies between arterioles of similar size from different vascular beds and also between vessels within a given microcirculatory bed (32). Among the possible reasons for this apparent functional het-
289 • OCTOBER 2005 •
www.ajpheart.org
MEMBRANE POTENTIAL AND CA2⫹ PATHWAYS IN MYOGENIC TONE
erogeneity is the variation in the extent and involvement of intracellular stores (33). In addition to differences relating to regional variation in vascular smooth muscle cells, differing results may be a function of protocols employed. For example, protocols involving long time periods of exposure of arterioles to 0 mM Ca2⫹, or decreases in intraluminal pressure, may compromise pressure-evoked Em signals and hence the development of tone when VOCCs are blocked. Whether the endothelium has been removed from a given preparation may also contribute to discrepancies. While the endothelium does not directly contribute to arteriolar myogenic tone under no-flow conditions, pharmacological interventions adopted to assess the role of various sources of Ca2⫹ would also have effects on the endothelial Ca2⫹ levels, thus modulating the release of endothelial vasoactive stimuli that in turn modulate smooth muscle Ca2⫹ levels and hence the level of tone. In addition, consideration must be given to drug specificity. For example, some inhibitors of VOCC may have additional effects on store-filling related mechanisms (4, 24). Compounding this, evidence exists to suggest that depolarization-induced Ca2⫹ entry may also contribute to store filling (2). In summary, the present study demonstrates a more complex relationship between Em and arteriolar tone than has been previously appreciated and is suggestive of heterogeneity between vascular beds. This relationship has significant physiological implications for responsiveness to stimuli that target Em and provides a base against which the hyperpolarizing influences of neural and endothelial origin can be predicted. Furthermore, the data may be relevant to understanding the action of hyperpolarizing vasodilators in pathophysiological states such as hypertension. ACKNOWLEDGMENTS Thanks are extended to Dr. Michael Davis for helpful discussions. GRANTS Studies described in this manuscript were supported by the National Health and Medical Research Council of Australia and the National Heart Foundation of Australia. REFERENCES 1. Bartlett IS, Crane GJ, Neild TO, and Segal SS. Electrophysiological basis of arteriolar vasomotion in vivo. J Vasc Res 37: 568 –575, 2000. 2. Bradley KN, Flynn ER, Muir TC, and McCarron JG. Ca2⫹ regulation in guinea-pig colonic smooth muscle: the role of the Na⫹-Ca2⫹ exchanger and the sarcoplasmic reticulum. J Physiol 538: 465– 482, 2002. 3. Brayden JE and Nelson MT. Regulation of arterial tone by activation of calcium-dependent potassium channels. Science 256: 532–535, 1992. 4. Curtis TM and Scholfield CN. Nifedipine blocks Ca2⫹ store refilling through a pathway not involving L-type Ca2⫹ channels in rabbit arteriolar smooth muscle. J Physiol 532: 609 – 623, 2001. 5. Davis MJ and Hill MA. Signaling mechanisms underlying the vascular myogenic response. Physiol Rev 79: 387– 423, 1999. 6. Davis MJ, Meininger GA, and Zawieja DC. Stretch-induced increases in intracellular calcium of isolated vascular smooth muscle cells. Am J Physiol Heart Circ Physiol 263: H1292–H1299, 1992. 7. Harder DR. Pressure-dependent membrane depolarization in cat middle cerebral artery. Circ Res 55: 197–202, 1984. 8. Haws CW and Heistad DD. Effects of nimodipine on cerebral vasoconstrictor responses. Am J Physiol Heart Circ Physiol 247: H170 –H176, 1984. 9. Hill CE, Eade J, and Sandow SL. Mechanisms underlying spontaneous rhythmical contractions in irideal arterioles of the rat. J Physiol 521: 507–516, 1999.
AJP-Heart Circ Physiol • VOL
H1333
10. Hill MA and Meininger GA. Calcium entry and myogenic phenomena in skeletal muscle arterioles. Am J Physiol Heart Circ Physiol 267: H1085– H1092, 1994. 11. Hill MA, Zou H, Davis MJ, Potocnik SJ, and Price S. Transient increases in diameter and [Ca2⫹]i are not obligatory for myogenic constriction. Am J Physiol Heart Circ Physiol 278: H345–H352, 2000. 12. Hill MA, Zou H, Potocnik SJ, Meininger GA, and Davis MJ. Invited review: arteriolar smooth muscle mechanotransduction: Ca2⫹ signaling pathways underlying myogenic reactivity. J Appl Physiol 91: 973–983, 2001. 13. Hwa JJ and Bevan JA. Dihydropyridine calcium agonists selectively enhance resistance artery myogenic tone. Eur J Pharmacol 126: 231–238, 1986. 14. Hwa JJ and Bevan JA. A nimodipine-resistant Ca2⫹ pathway is involved in myogenic tone in a resistance artery. Am J Physiol Heart Circ Physiol 251: H182–H189, 1986. 15. Jackson WF and Blair KL. Characterization and function of Ca2⫹activated K⫹ channels in arteriolar muscle cells. Am J Physiol Heart Circ Physiol 274: H27–H34, 1998. 16. Jaggar JH, Porter VA, Lederer WJ, and Nelson MT. Calcium sparks in smooth muscle. Am J Physiol Cell Physiol 278: C235–C256, 2000. 17. Jaggar JH, Wellman GC, Heppner TJ, Porter VA, Perez GJ, Gollasch M, Kleppisch T, Rubart M, Stevenson AS, Lederer WJ, Knot HJ, Bonev AD, and Nelson MT. Ca2⫹ channels, ryanodine receptors and Ca2⫹-activated K⫹ channels: a functional unit for regulating arterial tone. Acta Med Scand 164: 577–587, 1998. 18. Johnson P. The myogenic response. In: Handbook of Physiology. The Cardiovascular System. Vascular Smooth Muscle. Bethesda MD: Am. Physiol. Soc., 1980, sect. 2, vol. II, chapt. 15, p. 409 – 442. 19. Johnson PC. Autoregulation of blood flow. Circ Res 59:483– 495, 1986. 20. Knot HJ, Standen NB, and Nelson MT. Ryanodine receptors regulate arterial diameter and wall [Ca2⫹] in cerebral arteries of rat via Ca2⫹dependent K⫹ channels. J Physiol 508: 211–221, 1998. 21. Lagaud G, Gaudreault N, Moore ED, Van Breemen C, and Laher I. Pressure-dependent myogenic constriction of cerebral arteries occurs independently of voltage-dependent activation. Am J Physiol Heart Circ Physiol 283: H2187–H2195, 2002. 22. Ma HT, Patterson RL, van Rossum DB, Birnbaumer L, Mikoshiba K, and Gill DL. Requirement of the inositol trisphosphate receptor for activation of store-operated Ca2⫹ channels. Science 287: 1647–1651, 2000. 23. Mauban JR, Lamont C, Balke CW, and Wier WG. Adrenergic stimulation of rat resistance arteries affects Ca2⫹ sparks, Ca2⫹ waves, and Ca2⫹ oscillations. Am J Physiol Heart Circ Physiol 280: H2399 –H2405, 2001. 24. McCarron JG, Flynn ER, Bradley KN, and Muir TC. Two Ca2⫹ entry pathways mediate InsP3-sensitive store refilling in guinea-pig colonic smooth muscle. J Physiol 525: 113–124, 2000. 25. Meininger GA, Zawieja DC, Falcone JC, Hill MA, and Davey JP. Calcium measurement in isolated arterioles during myogenic and agonist stimulation. Am J Physiol Heart Circ Physiol 261: H950 –H959, 1991. 26. Murphy TV, Kotecha N, and Hill MA. Effect of increased myogenic tone on endothelium-dependent dilation of arterioles (Abstract). FASEB J 16: A79, 2002. 27. Neild TO. Measurement of arteriole diameter changes by analysis of television images. Blood Vessels 24: 452– 485, 1989. 28. Nelson MT, Cheng H, Rubart M, Santana LF, Bonev AD, Knot HJ, and Lederer WJ. Relaxation of arterial smooth muscle by calcium sparks. Science 270: 633– 637, 1995. 29. Osol G, Brekke JF, McElroy K, and Gokina NI. Myogenic tone, reactivity, and forced dilatation: a three-phase model of in vitro arterial myogenic behavior. Am J Physiol Heart Circ Physiol 283: H2260 –H2267, 2002. 30. Potocnik SJ and Hill MA. Pharmacological evidence for capacitative Ca2⫹ entry in cannulated and pressurized skeletal muscle arterioles. Br J Pharmacol 134: 247–256, 2001. 31. Prakriya M and Lewis RS. Potentiation and inhibition of Ca2⫹ releaseactivated Ca2⫹ channels by 2-aminoethyldiphenyl borate (2-APB) occurs independently of IP3 receptors. J Physiol 536: 3–19, 2001. 32. Schubert R and Mulvany MJ. The myogenic response: established facts and attractive hypotheses. Clin Sci 96: 313–26, 1999.
289 • OCTOBER 2005 •
www.ajpheart.org
H1334
MEMBRANE POTENTIAL AND CA2⫹ PATHWAYS IN MYOGENIC TONE
33. Somlyo AP and Himpens B. Cell calcium and its regulation in smooth muscle. FASEB J 3: 2266 –2276, 1989. 34. Sugawara H, Kurosaki M, Takata M, and Kurosaki T. Genetic evidence for involvement of type 1, type 2 and type 3 inositol 1,4,5trisphosphate receptors in signal transduction through the B-cell antigen receptor. EMBO J 16: 3078 –3088, 1997. 35. Watanabe J, Karibe A, Horiguchi S, Keitoku M, Satoh S, Takishima T, and Shirato K. Modification of myogenic intrinsic tone and [Ca2⫹]i of rat
AJP-Heart Circ Physiol • VOL
isolated arterioles by ryanodine and cyclopiazonic acid. Circ Res 73: 465– 472, 1993. 36. Wesselman JP, Schubert R, VanBavel ED, Nilsson H, Mulvany MJ. KCa-channel blockade prevents sustained pressure-induced depolarization in rat mesenteric small arteries. Am J Physiol Heart Circ Physiol 272: H2241–H2249, 1997. 37. Zou H, Ratz PH, and Hill MA. Role of myosin phosphorylation and [Ca2⫹]i in myogenic reactivity and arteriolar tone. Am J Physiol Heart Circ Physiol 269: H1590 –H1596, 1995.
289 • OCTOBER 2005 •
www.ajpheart.org