Australian bat lyssavirus. ABLV. NC_003243.1. Aravan virus. ARAV. EF614259.1. Adelaide River virus. ARV. JN935380.1. Bas-Congo virus. BASV. JX297815.1.
SUPPLEMENTARY ONLINE DATA
Supplementary Table S1: Viruses and GenBank accession numbers used in phylogenetic analysis
VIRUS Almpiwar virus Australian bat lyssavirus Aravan virus Adelaide River virus Bas-Congo virus Berrimah virus Bovine ephemeral fever virus Chandipura virus Coastal Plains virus Cocal virus Drosophilia affinis sigma virus Drosophilia melanogaster sigma virus AP30 Drosophilia melanogaster sigma virus HAP23 Drosophilia obscura sigma virus Durham virus Duvenhage virus European bat lyssavirus 1 European bat lyssavirus 2 Eel virus European X Flanders virus Gray Lodge virus Harrison Dam virus Infectious haematopoietic necrosis virus Irkut virus Isfahan virus Joinjakaka virus Kamese virus Khujand lyssavirus Kimberley virus Kolente virus Kotonkan virus La Joya virus Lagos Bat virus Landjia virus Lettuce necrotic yellows rhabdovirus Lettuce yellow mottle virus Manitoba virus Maraba virus Marco virus Maize mosaic virus Mokola virus Mosqueiro virus Mossuril virus Moussa virus North cereal mosaic virus Ngaingan virus Niakha virus North Creek virus Oak Vale virus 1342
Supplementary Table S2: HOJV and ORRV transcription and replication control sequences
Junction leader – N(U4)
N(U4) – P
P – U1
U1 – U2
U2 – U3
U3 – M
M – G(U5)
G(U5) – L
Gene End
IGR
Gene Start
HOJV
n.a.
n.a.
AGTAGTTCTTATGACTACATCTCAAATCAACAATG
ORRV
n.a.
n.a.
AGTAGTTCTTATGACTACTTAACCAAGCAAAATG
WONV
n.a.
n.a.
AGTAGTTTTCATGACTACATCTCCAAAAACAATG
HOJV*
TAA…104 nt…CATGAAAAAAA
TC
AGTAGTTATCATG
ORRV
TAA…131 nt…CATGAAAAAAA
TC
AGTAGTTATTATG
WONV
TAA…131 nt…CATGAAAAAAA
TC
AGTAGTTATCATG
TAAACCATGAAAAAAA
TC
AGTAGACATCTAGTCTACAAGATG
ORRV
TAAAACATGAAAAAAA
TC
AGTAGATATCATATCTACACCATG
WONV
TGATCACATGAAAAAAA
TC
AGTAGACATCTAGTCTACAAGATG
HOJV
TGATCATGAAAAAAA
TC
GGCAGTCATCATG
ORRV
TAGATCATGAAAAAAA
TC
GGCAGTCATCATG
WONV
TAATACATGAAAAAAA
TC
GGCAGTCATCATG
HOJV
TAAATCATGAAAAAAA
TC
AGTAGCAATCATG
ORRV
CATGAAAAAAA
TT
AGTAGACATCATG
WONV
TAAATCTTGAAAAAAA
TC
AGTAGTCATCATG
HOJV
TGATCATGAAAAAAA
CT
AGCAGAAATCATG
ORRV
TGACACATGAAAAAAA
TT
AGCAGCAATCATG
WONV
TAAATACATGAAAAAAA
TC
AGCAGTCATCATG
HOJV
TAACATGAAAAAAA
CC
AGTAGACATATTGAATACAAAATG
ORRV
TAACATGAAAAAAA
TT
AGTAGACATATTGTCTAATTTTTTGAAAATG
WONV
TAACATGAAAAAAA
CT
AGTAGACATATTGATTACAAGATG
HOJV
CATGAAAAAAA
CC
AGTAGGCATTATG
ORRV
CATGAAAAAAA
CT
AGTAGACATAATG
CT
AGCAGTCATAATG
HOJV
CTTGAAAAAAA
WONV L – trailer
HOJV
TAA…29 nt…CATGAAAAAAA
n.a.
n.a
ORRV
TAA…29 nt…CATGAAAAAAA
n.a.
n.a.
WONV
TAA…28 nt…CATGAAAAAAA
n.a.
n.a.
Stop and start codons are bolded and underlined, putative transcription start signals are bolded, and the transcription stop/polyadenylation signals are underlined. IGR, intergenic region. Remnant/nonfunctional or alternate translation start signals in the HOJV and ORRV N proteins, and a premature stop signal in the ORRV N protein are in grey type. (*) HOJV does not contain a U4 ORF. In ORRV and WONV, the first marked stop codon relates to the N ORF and the second marked stop codon (which overlaps the transcription stop signal in both viruses) relates to the U4 ORF. Brackets indicate overlapping ORFs.
Supplementary Figure S1: Multiple (MUSCLE) sequence alignment of the M proteins of HOJV, ORRV and WONV. Fully conserved (*), strongly conserved (:) and weakly conserved (.) amino acids are marked. Five in-frame methionine residues conserved between all three viruses at positions 57, 106, 170, 180 and 192, which may be suggestive of the generation of shorter M protein translation products, are marked in red.
