NE C8 121 ST 122 ST 120 ST 123 ST 133 ST 135 ST DW 143 ST 145

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Supplementary Figure 1. NE. C8. 121 S. T. 122 S. T. 120 S. T. 123 S. T. 133 S. T. 135 S. T. DW. 143 S. T. 145 S. T. 140 S. T. 158 S. T. 170 S. T. 150 S. T. 117 S. T.
DW

170 ST

158 ST

150 ST

145 ST

143 ST

140 ST

135 ST

123 ST 133 ST

121 ST 122 ST

120 ST

117 ST 119 ST

NEC8

M

DNMT3L

β-ACTIN

Supplementary Figure 1

+D NM T3L He La NE C8 NE C1 4 hE S

2 93

DNMT3L SOX2 β-ACTIN

Supplementary Figure 2

Supplementary figure legends. Supplementary Fig. 1. Expression of DNMT3L mRNA in testicular tissues adjacent to the TGCTs. RT-PCR analysis of DNMT3L in testicular tissues adjacent to the TGCTs. The bottom lane shows RT-PCR results using β-actin primers as a control. ST: surrounding testicular parenchyma tissues adjacent to the TGCTs. The number of each tissue represents the serial code number of our laboratory corresponding to the primary TGCT tissues.

Note that the transcripts of DNMT3L were

negative in the testicular tissues adjacent to the germ cell tumors while the EC cell line (NEC8) shows expression of DNMT3L. M, molecular weight marker. DW, distilled water as a negative control for PCR.

Supplementary Fig. 2. Comparative expression of DNMT3L and SOX2 proteins in EC cell lines and human embryonic stem (hES) cells. Western blots with DNMT3L and SOX2 antibodies are shown. Note that both DNMT3L and SOX2 proteins were expressed in hES cells as well as human EC cell lines.