Supplementary Figure 1. NE. C8. 121 S. T. 122 S. T. 120 S. T. 123 S. T. 133 S. T. 135 S. T. DW. 143 S. T. 145 S. T. 140 S. T. 158 S. T. 170 S. T. 150 S. T. 117 S. T.
DW
170 ST
158 ST
150 ST
145 ST
143 ST
140 ST
135 ST
123 ST 133 ST
121 ST 122 ST
120 ST
117 ST 119 ST
NEC8
M
DNMT3L
β-ACTIN
Supplementary Figure 1
+D NM T3L He La NE C8 NE C1 4 hE S
2 93
DNMT3L SOX2 β-ACTIN
Supplementary Figure 2
Supplementary figure legends. Supplementary Fig. 1. Expression of DNMT3L mRNA in testicular tissues adjacent to the TGCTs. RT-PCR analysis of DNMT3L in testicular tissues adjacent to the TGCTs. The bottom lane shows RT-PCR results using β-actin primers as a control. ST: surrounding testicular parenchyma tissues adjacent to the TGCTs. The number of each tissue represents the serial code number of our laboratory corresponding to the primary TGCT tissues.
Note that the transcripts of DNMT3L were
negative in the testicular tissues adjacent to the germ cell tumors while the EC cell line (NEC8) shows expression of DNMT3L. M, molecular weight marker. DW, distilled water as a negative control for PCR.
Supplementary Fig. 2. Comparative expression of DNMT3L and SOX2 proteins in EC cell lines and human embryonic stem (hES) cells. Western blots with DNMT3L and SOX2 antibodies are shown. Note that both DNMT3L and SOX2 proteins were expressed in hES cells as well as human EC cell lines.