Veterinary World, EISSN: 2231-0916 Available at www.veterinaryworld.org/Vol.10/March-2017/8.pdf
RESEARCH ARTICLE Open Access
Molecular characterization of velogenic viscerotropic Ranikhet (Newcastle) disease virus from different outbreaks in desi chickens V. S. Dhaygude1, G. K. Sawale1, M. M. Chawak2, N. R. Bulbule2, S. D. Moregaonkar1 and D. S. Gavhane1 1. Department of Veterinary Pathology, Bombay Veterinary College, Parel, Mumbai, Maharashtra, India; 2. Department of Veterinary Pathology, Poultry Diagnostic and Research Centre, Lono Kalbhor, Pune, Maharashtra, India. Corresponding author: V. S. Dhaygude, e-mail:
[email protected], Co-authors: GKS:
[email protected], MMC:
[email protected], NRB:
[email protected], SDM:
[email protected], DSG:
[email protected] Received: 26-11-2016, Accepted: 07-02-2017, Published online: 17-03-2017 doi: 10.14202/vetworld.2017.319-323 How to cite this article: Dhaygude VS, Sawale GK, Chawak MM, Bulbule NR, Moregaonkar SD, Gavhane DS (2017) Molecular characterization of velogenic viscerotropic Ranikhet (Newcastle) disease virus from different outbreaks in desi chickens, Veterinary World, 10(3): 319-323.
Abstract Aim: Diagnosis of velogenic viscerotropic Ranikhet disease from six different flocks of desi chicken in and around Mumbai by gross and histopathological examination, isolation of virus and molecular methods. Materials and Methods: A total of 25 carcasses (varying between 2 and 6 carcasses from each flock) of six different flocks of adult desi chicken were subjected to necropsy examination for diagnosis of the disease during the span of a year (2014-2015). After thorough gross examination, the tissue samples were collected and processed for virus isolation and histopathological examination. The 20% tissue homogenate was inoculated into 9-day-old specific pathogen free (SPF) embryonated eggs. Mean death time (MDT) of embryos after inoculation and intracerebral pathogenicity index (ICPI) were used to judge velogenic nature of the virus. Newcastle disease virus (NDV) was isolated from six cases and confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) targeting the partial fusion protein gene of the viral genome. Results: A total of 25 carcasses (varying between 2 and 6 carcasses from each flock) of six different flocks of desi chicken were presented for postmortem examination to Department of Veterinary Pathology, Bombay Veterinary College, Parel, Mumbai during 2014-2015. The gross and histopathological examination revealed lesions suggestive of viscerotropic velogenic form of the Newcastle disease (ND). The 20% tissue homogenate was inoculated into 9-day-old embryonated eggs from SPF chicken. NDV was isolated from six cases and confirmed by RT-PCR targeting the partial fusion protein gene. MDT of all the isolates was
