the frequently used diaminofluorescein (DAF-2 and. DAF-2DA) and by
chemiluminescence were compared using the following systems of increasing
complexity:.
Journal of Experimental Botany, Vol. 57, No. 12, pp. 3043–3055, 2006 doi:10.1093/jxb/erl070 Advance Access publication 7 August, 2006
RESEARCH PAPER
Nitric oxide (NO) detection by DAF fluorescence and chemiluminescence: a comparison using abiotic and biotic NO sources Elisabeth Planchet and Werner M. Kaiser* Julius-von-Sachs Institute for Biosciences, University of Wu¨rzburg, Julius-von-Sachs-Platz 2, D-97082 Wu¨rzburg, Germany Received 20 March 2006; Accepted 30 May 2006
Abstract Because of controversies in the literature on nitric oxide (NO) production by plants, NO detection by the frequently used diaminofluorescein (DAF-2 and DAF-2DA) and by chemiluminescence were compared using the following systems of increasing complexity: (i) dissolved NO gas; (ii) the NO donor sodium nitroprusside (SNP); (iii) purified nitrate reductase (NR); and (iv) tobacco cell suspensions. Low (physiological) concentrations (
