May 21, 1991 - A novel method has been developed to quantify a(1-*3)-. L-fucosyltransferase activity in human sera by applying a sandwich-type ...
CLIN.CHEM.37/12, 2081-2086 (1991)
Novel Method for Quantifying a(1-3)-L-Fucosyltransferase Activity in Serum Tetsuya Tachikawa,’
Shin Yazawa,2 Takayuki
Asao,3 Sadahito
Shin,’
and Noboru
Yanalhara4
A novel method has been developed to quantify a(1-*3)L-fucosyltransferase activity in human sera by applying a sandwich-type immunoradiometric assay. H type 2 trisaccharide (8Fuca1-*2GaI1-4GlcNAc) covalently attached to bovine serum albumin (BSA) was used as an acceptor and incubated with serum samples in the presence of guanosine diphosphate-fucose. The resulting product,Ytetrasacchande (Fucal-*2Gal(31--+4[Fucal-+3] GIcNAcf3-BSA), was detected by a sequential use of anti-BSA antibody-coated bead and 1l-labeled anti-V antibody. Inter- and intra-assay CVs for a(1-+3)-L-fucosyltransferase were both
