Nuclear reprogramming and development of handmade cloned porcine embryos induced by Trichostatin A
Siriboon Chawalit1, Ngoc Tan Nguyen1, Jung-Kai Tseng2, Neng-Wen Lo3, Chin-Fu Tu4, and Jyh-Cherng Ju1,5
5
1
Department of Animal Science, National Chung Hsing University, 250 Kuokuang
Road, Taichung 402, Taiwan, ROC 2
10
School of Optometry, Chumg Shan Medical University, 110 Chien-Kuo North Road,
Taichung 402, Taiwan, ROC. 3
Department of…Animal Science and Biotechnology, Tunghai University. 181, Sec.
3, Taichung Harbor Rd., Taichung 407, Taiwan, ROC. 4
Animal Technology Institute Taiwan, 52 Kedung 2 Rd., Ding-Pu LII, Chunan,
Miaoli, Taiwan, ROC. 15
5
Correspondence: Dr. Jyh-Cherng Ju, professor
E-mail:
[email protected]
20 Running title: Reprogramming and development of HMC porcine embryos
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1
Abstract Histone modification of specific nuclear activities is an essential process of transcriptional regulation during embryonic development and is generally associated 30
with the level of gene expression. The aim of this study was to improve handmade cloning efficiency or developmental competence of porcine handmade cloned (HMC) embryos by Trichostatin A (TSA), a histone deacetylation inhibitor, treatment. Reconstructed embryos were treated with 0, 20, 30, or 40 nM TSA for 24 h after parthenogenetic activation. Blastocyst rates of the reconstructed embryos reached as
35
much as in close proximity to 60% in both the 30 and 40 nM TSA treatment groups, which were significantly higher (P
