Nucleotide sequence of a novel crystal protein gene isolated from ...

Nucleic Acids Research, Vol. 18, No. 24 7443

Nucleotide sequence of a novel crystal protein gene isolated from Bacillus thuringiensis subspecies kenyae M.Bosse, L.Masson and R.Brousseau* Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Ave, Montreal, Quebec H4P 2R2, Canada Submitted October 30, 1990

EMBL accession

A novel protoxin gene from Bacillus thuringiensis subspecies kenyae, encoding a 134 kilodalton protein toxic for Bombyx mori, has been isolated. The protoxin shares amino acid homology to both crylA and crylC protoxin subclasses (80-83%) and to the toxin (amino acids 1-650; 70-72%). However, the C-terminus of the activated toxins (amino acids 300-650) shows more sequence similarity to the cryIC (75%) than the cryIA (61-63%) 1 91 181 271 361 451 541 631 721

ATTA

classes. Therefore, until more extensive studies are performed on the pathotype of this protoxin, we tentatively place this gene in the new classification scheme (1)

*

as a

cryIC(b) gene.

REFERENCE 1. Hofte,H. and Whiteley,H.R. (1989) Microbiol. Rev. 53, 242-255.

90

TCTCTGCTCtCTTCCGCACTCA ATAGGGGGGATT IATTAGGATTGTTTGAT

180

TTGATTGACCAAAAAATAGAGAATTCGCT GCTTTAGAGAGTGGGACATCCTACT ACAGCTATTCCTCTTirT?CAGTTCAAAAT

270 360

GCAATATGGGGGTCTATAGGCCCTTCACM TGGGATTTATTTTTAGAGCAAATTGAGCTA AGAAACCAGGCAATTTCTAGATTAGAAGGG ATAAGCAGTCTTACGGAATTTATCAGAA AATCCAGCATTAAAAGAAGATGCGTACT CAATTTAATGACATGAACAGTATTCTTGTA TATCAAGTCCCATTTTTATCAGTATATGTT CAAGCTGCAATTTACATTTATCGGTTTTG GGATTTGATATAGCAACAATAAATAGTCGT TATAATGATCTGACTAGACTTATTCCTATA ACCGCATTAGATCXTTACCACGAACTGCT GGGCTGCGAACTGGGCAAGA?TTMTCAG GATATTATTTC?TTtTTCAGAATTACGAT TCTAGATTATATCCAATTCCAACAAGCTCC 811 GTAATTAATATAACTGACTATAGAGTTGOC CCCAGCTCGAGAATATTGAGAACTCAQC 901 AATAATTTGACCATTGATACGGATTTGATT AGAGGTCrTCACTATTGGGCAGCATCGT 991 GTGATAACMCCCCTCAATATGGGATAACC QCAMTWAGACCAAGACGAACTAMTC'TC 1081 ATAGMCATTATCAAATCCTTTCTTCCGAA GATCAGAAAAATATTACTCCTACCTTAGG 1171 1261 1351 1441 1531 1621 1711 1801 1891 1981 2071 2161 2251 2341 2431 2521 2611 2701 2791 2881 2971 3061 3151 3241 3331 3421 3511

AGAGATG CAGTGTTGGGCAGGCTTGG TATACAGATTATGCTGTACGCTGTACAAT TTTAGAAGAGAGTTAACAATATCAGTATTA CAATTAACGCGGGAAGTATATACGATCCG ATTAGAAGCCCCCACCTTAT ACIA GTAACTTCCACATACAGGTAGCTCAA

450 540 630

720 610

900 990

CTMTACGTLTCAGGTCTTAACCTATTTT ATAAATGTAGGTACAGGGATAATGTAGTA

100 1170

CTAGATTCTCTThATGAGTTACCAATTGAT CAGGCAGTAGGGTTCATTCAACCAAATAAT GCTGAAGTCrATATAGAAGTAGGCG GGTGAGAATTCATTAGTTGGATATAGTCAT CGATTAAGTCATGTTACACTAACCAGGT=G TTATATAATACTAATATAACTACCTGCCA ACATTTGTTTGGACACATCACAGTGCTACT AATACAAATACAATTMTCAGATATTATT ACACAAATACCiTrAGTGAAAGATTTAGA

1260

CTTGGTGGTGGCACCTCTGTCATTACAGGA CAAGTCAATATTAACTCACCArTTACCCAA GGAGGACAAATTAGAGTAGATATGACCCTT AGTAATCCmTTTTCATTTAGGGTTAATCCA

CCAGGATTTACAGGACGGATATCMCCA AGAAATACCATGGTGAGTTTCrGrCTTTA AGATACCCrrrAGATTrrCATGCTYC AGTAGGGATGCACGAATTAC=TGACCGATA

GAAAAAACCATGGAXAkTGCGGAGAGC7TA ACATCTAGAACATTTAGCTATACAATTTT GATATAATTAGAATACTGAAGAACTTCCT ATTCGGTGGTGAGCTTTATATAGATAAA ATTGACTTATTCTAGCAGATGCAACATmT GAAGAAGAATATG&TTTGGAAAGAOCAChG AA(GGCXTGAATGGCCTGTTACTTCTACA AATCAACTAGGGCTAAAAACAGATGTGACG GATTATCATATTGATCAACrTTTCCAATTTA GTTGAGTGTTTATCGGATGAATTTTGTCTG GATGAAAAGAGAGATTATCCGAGAAAGTC AAACATGCGAAGCGACTCAGTGATGAACG AATTTACTTCAAGATCCAACITCAGAGG ATCAATAGGCAACCAGACCGTTGCTGGAGA GGAAGCACGGATATTACTATCCAATA GATGACGTATTCAAAGAGAATTACGTCACA TTACCGGGTACCTTTGATGAGTGCTATCCA ACGTATTTATATCAAMATAGATGAGTCG AAGTTAAAAGCTTATACCCGCTATGATTA AGAGCTATATCGAGGATAGTCAAGACTCA GAAATCTAT7TAATTCCTACAATGCAAAA CACGAGACAGTAAACGTGCCGGTACGGGT TCCTTATGGCCGC7TTCAGCCCAAAGTCCA ATCGGAAAGTGTGGAGACCGAATCATGC GCXCACACCTTGAATGGAATCCTAATCTA GATTCTCCTGCAGAGACGGGAAAMTGT GCCCATCATTCCCATCATTrCTCCTTGGAC ATTGATGTTGGATGTACAGACTTAAATGAG CGTSAGGTGTATGGGTGATATTCAAGATT MGACACAAGATGGCTATGCAACTAGGA AATCTAGAGTTTCTCGAAGAGAAACCACTA TTAGGGAAGCACTAGCTCGTGTGAAAAGA GCGGAGAAAAATGGAGAGACAAATGCGAA AAATTGGAATGGGAAACAAATATTGTTTAT AAAGAGGCAAAAGAATCTGTAGATGCTTTA TTTGTAAACTTCAATATGATAGATTACAA GCGGATACGAATATCGCG&TG&TTCATGCG GCAGATAAACGCGTTCATAGCATTCGAGMA GCGTATCTGCCAGAGCUTGTCGTGATTCCG GGTGTCAATGCGGCTATTTTTGAAGAATTA GAAGGGCGTATTTCACTGCATTCTCCCTA TATGATGGAGAAATGTCATTAAAAATSC GATTTcATAATGGCTTATCATOCTGGAAC GAATGGGAGCAGAAGTGTCACAGAAGTT GTGAAAGGGCATGTAGATGTAGAAGAACAG AACAACCATTTCL CCTGTTTGTCCGGGTCGTGGCTATATCCTT CGTGTTACAGCGTACAAAG&GGGATATGGA GAGGGCYGTGTAACGATTCATGAGATCGAA GACAATACAGACGAACTGAAATTCAGCAAC TGTGTAGAAGAGGAAGTATATCCAAACAC ACGGTAACGTGTAATAATTATAhGCGACT CMGAAGAACATGAGGGTACGTACACTTCC CGTMTCGAGGATATGACGAAGCCTATGAA AGCAATTCr'GTACATGCGCAGTCTAT GAAGAAAAATCGTATACAGATAGACGAAGA GAGAATCCTTGTGAATCTAACAGAGGATAT GGGGATTACACACCACTACCAGCrGCYTAT GTGACAAAAGAATTAGAGTACTTCCCAGAA ACCGATAAGGTATGGATTGAGATCGtAGAA ACGGMGGAACATTCATCGTGGACAGCGTG GAATTACTTCTTATGGAGGAATAAtatata tgctttaaatgt 3552

To whom correspondence should be addressed

X56144

gene

ATGGAGATAGTGATAATCAGATCAATXC GTGCCTTATAATTGTTAAATAATCCTGAA AATGAGATATTAGATATT'GAAA TCAAAT AGTACTGTAGCAACAAACATCGccrGGA

no.

1350 1440 1530 1620 1710 1800 1890 1960

2070 2160 2250 2340 2430 2520 2610 2700 2790 2880 2970 3060

3150 3240 3330 3420 3510