N E W S
A N D
V I E W S
Obesity: A Somatotrope Perspective Buffy S. Ellsworth Department of Physiology, Southern Illinois University in Carbondale, Carbondale, Illinois 62901-6523
ith the obesity epidemic increasing steadily in the United States, learning how energy homeostasis is regulated is imperative (1, 2). Leptin has been a molecule of great interest for this reason. The road to leptin discovery began in 1950 at Jackson Laboratories when a spontaneous mutation arose resulting in hyperphagia and severe obesity (3). The mutation was named the obese mutation, and Ob/Ob mice were the focus of decades of research. The gene encoding leptin was finally cloned in 1994 (4). Its receptor was cloned the year after (5). Leptin acts as an indicator of energy stores and regulates appetite through regulation of orexogenic (NPY and AgRP) and anorexogenic (␣MSH) signals in the hypothalamus (6). Although mutations in leptin and its receptor are associated with severe obesity in mice, rats, and humans (3–5, 7, 8), these mutations account for a very small fraction of human obesity cases (9 –13). Even so, learning more about leptin and its role in energy homeostasis promises to provide researchers and health professionals with important information about obesity. Sensing energy stores is important for growth and metabolism, which need to be closely linked to nutrition. Somatotrope cells in the anterior pituitary gland secrete GH, which regulates growth and body mass through stimulation of long bone growth, muscle anabolism, and lipolysis (14, 15). Thus, GH decreases fat and increases muscle mass. Several nutrition-sensing hormones regulate somatotrope function, including ghrelin, insulin, and leptin (16 –19). Although many studies aimed at understanding obesity have focused on the role of ghrelin, a potent secretagogue for GH, very few have ventured downstream to the somatotrope cells (20 –23). Ghrelin is released by distinct endocrine cells of the stomach in response to fasting and is important for energy homeostasis (16). Leptin, which is produced by adipose tissue and by the pituitary,
W
acts as a satiety factor in the hypothalamus and can stimulate GH production (14, 17). Over 80% of somatotrope cells express the receptor for leptin, Leprb (24). Ob/Ob and Db/Db mice, which lack leptin signaling, have reduced somatotrope numbers and reduced serum GH levels (15, 17). Whether the effect of leptin on somatotrope function is direct or indirect due to increased adipose tissue, metabolic disease, or hypogonadism has been difficult to ascertain. The paper by Syed et al in this issue of Endocrinology addresses the role of the leptin receptor in somatotrope function. The authors employed a Gh-Cre mouse (25) to delete the long form of the leptin receptor (Leprb) specifically in somatotrope cells (30). Somatotrope-specific Leprb deletion mutants exhibit a reduction in serum GH levels leading to reduced fat burning and changes in adipokines resulting in obesity by 5– 6 months of age (26 –28). Syed et al find that the number of somatotrope cells in leptin receptor deletion mutants is normal based on in situ hybridization for Gh mRNA but that most these somatotrope cells do not contain enough GH to be detected by immunohistochemistry, suggesting that somatotrope cells are not functioning normally. The authors demonstrate that the failure in somatotrope function is due to reduced sensitivity to GHRH. However, treatment with both GHRH and ghrelin restored GHRH sensitivity, increased the number of GH-immunoreactive cells to normal proportions, and increased serum GH to normal levels (30). These studies suggest that leptin receptor is necessary for maintaining somatotrope sensitivity to GHRH and that ghrelin is key for this process. This is valuable mechanistic information about how leptin signaling regulates somatotrope function to affect energy homeostasis. Syed et al answer several key questions about the interplay between metabolism and somatotrope function and uncover many more questions that need to be ad-
ISSN Print 0013-7227 ISSN Online 1945-7170 Printed in U.S.A. Copyright © 2013 by The Endocrine Society For article see page 1565
1390
endo.endojournals.org
Endocrinology, April 2013, 154(4):1390 –1391
doi: 10.1210/en.2013-1159
doi: 10.1210/en.2013-1159
dressed. These studies were performed in primary pituitary cells from Leprb deletion mutants (30). How would ghrelin and GHRH affect GH levels in whole-animal studies of Leprb deletion mutants? In-depth imaging of pituitary glands reveal 3-dimensional organization of somatotrope cells. Studies of intact animals would preserve the somatotrope networks that are present in intact pituitary glands, which facilitate GH secretion in response to secretagogue (29). The studies by Syed et al present clear evidence that ghrelin and leptin are important for GHRH sensitivity of somatotrope cells, leading us to wonder at the mechanism by which ghrelin and leptin signaling pathways interact to augment GHRH stimulation of GH production. Future studies that continue to elucidate the interplay between energy stores and somatotrope function will add to our understanding of how somatotrope cells sense and respond to changes in energy levels.
endo.endojournals.org
11.
12. 13.
14.
15.
16. 17. 18.
19.
Acknowledgments 20.
Address all correspondence and requests for reprints to: Buffy S. Ellsworth, PhD, Department of Physiology, Southern Illinois University in Carbondale, 1135 Lincoln Drive, Carbondale, Illinois 62901-6523. E-mail:
[email protected]. Disclosure Summary: The author has nothing to disclose.
22.
References
23.
1. Wang Y, Beydoun MA, Liang L, Caballero B, Kumanyika SK. Will all Americans become overweight or obese? Estimating the progression and cost of the US obesity epidemic. Obesity (Silver Spring). 2008;16:2323–2330. 2. Wang YC, McPherson K, Marsh T, Gortmaker SL, Brown M. Health and economic burden of the projected obesity trends in the USA and the UK. Lancet. 2011;378:815– 825. 3. Ingalls AM, Dickie MM, Snell GD. Obese, a new mutation in the house mouse. J Hered. 1950;41:317–318. 4. Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM. Positional cloning of the mouse obese gene and its human homologue. Nature. 1994;372:425– 432. 5. Tartaglia LA, Dembski M, Weng X, et al. Identification and expression cloning of a leptin receptor, OB-R. Cell. 1995;83:1263–1271. 6. Penicaud L, Meillon S, Brondel L. Leptin and the central control of feeding behavior. Biochimie. 2012;94:2069 –2074. 7. Sharma K, McCue P, Dunn SR. Diabetic kidney disease in the db/db mouse. Am J Physiol Renal Physiol. 2003;284:F1138 –F1144. 8. Ghilardi N, Ziegler S, Wiestner A, Stoffel R, Heim MH, Skoda RC. Defective STAT signaling by the leptin receptor in diabetic mice. Proc Natl Acad Sci USA. 1996;93:6231– 6235. 9. Farooqi IS, Wangensteen T, Collins S, et al. Clinical and molecular genetic spectrum of congenital deficiency of the leptin receptor. N Engl J Med. 2007;356:237–247. 10. Gotoda T, Manning BS, Goldstone AP, et al. Leptin receptor gene
21.
24.
25.
26.
27.
28.
29.
30.
1391
variation and obesity: lack of association in a white British male population. Hum Mol Genet. 1997;6:869 – 876. Karvonen MK, Pesonen U, Heinonen P, et al. Identification of new sequence variants in the leptin gene. J Clin Endocrinol Metab. 1998; 83:3239 –3242. Carlsson B, Lindell K, Gabrielsson B, et al. Obese (ob) gene defects are rare in human obesity. Obes Res. 1997;5:30 –35. Lucantoni R, Ponti E, Berselli ME, et al. The A19G polymorphism in the 5⬘ untranslated region of the human obese gene does not affect leptin levels in severely obese patients. J Clin Endocrinol Metab. 2000;85:3589 –3591. Asada N, Takahashi Y, Honjo M. Effects of 22K or 20K human growth hormone on lipolysis, leptin production in adipocytes in the presence and absence of human growth hormone binding protein. Horm Res. 2000;54:203–207. Isozaki O, Tsushima T, Miyakawa M, Demura H, Seki H. Interaction between leptin and growth hormone (GH)/IGF-I axis. Endocr J. 1999;46(suppl):S17–S24. Kojima M, Kangawa K. Ghrelin: structure and function. Physiol Rev. 2005;85:495–522. Popovic V, Damjanovic S, Dieguez C, Casanueva FF. Leptin and the pituitary. Pituitary. 2001;4:7–14. Luque RM, Kineman RD. Impact of obesity on the growth hormone axis: evidence for a direct inhibitory effect of hyperinsulinemia on pituitary function. Endocrinology. 2006;147:2754 –2763. Melmed S, Neilson L, Slanina S. Insulin suppresses rat growth hormone messenger ribonucleic acid levels in rat pituitary tumor cells. Diabetes. 1985;34:409 – 412. Tschop M, Weyer C, Tataranni PA, Devanarayan V, Ravussin E, Heiman ML. Circulating ghrelin levels are decreased in human obesity. Diabetes. 2001;50:707–709. Hansen TK, Dall R, Hosoda H, et al. Weight loss increases circulating levels of ghrelin in human obesity. Clin Endocrinol (Oxf). 2002;56:203–206. Yildiz BO, Suchard MA, Wong ML, McCann SM, Licinio J. Alterations in the dynamics of circulating ghrelin, adiponectin, and leptin in human obesity. Proc Natl Acad Sci USA. 2004;101:10434 – 10439. Schellekens H, Finger BC, Dinan TG, Cryan JF. Ghrelin signalling and obesity: at the interface of stress, mood and food reward. Pharmacol Ther. 135:316 –326. Childs GV, Akhter N, Haney A, et al. The somatotrope as a metabolic sensor: deletion of leptin receptors causes obesity. Endocrinology. 2010;152:69 – 81. Luque RM, Amargo G, Ishii S, et al. Reporter expression, induced by a growth hormone promoter-driven Cre recombinase (rGHpCre) transgene, questions the developmental relationship between somatotropes and lactotropes in the adult mouse pituitary gland. Endocrinology. 2007;148:1946 –1953. Ahima RS, Prabakaran D, Flier JS. Postnatal leptin surge and regulation of circadian rhythm of leptin by feeding. Implications for energy homeostasis and neuroendocrine function. J Clin Invest. 1998;101:1020 –1027. Attig L, Larcher T, Gertler A, Abdennebi-Najar L, Djiane J. Postnatal leptin is necessary for maturation of numerous organs in newborn rats. Organogenesis. 2011;7:88 –94. Akhter N, Odle AK, Allensworth-James ML, et al. Ablation of leptin signaling to somatotropes: changes in metabolic factors that cause obesity. Endocrinology. 2012;153:4705– 4715. Le Tissier PR, Hodson DJ, Lafont C, Fontanaud P, Schaeffer M, Mollard P. Anterior pituitary cell networks. Front Neuroendocrinol. 2012;33:252–266. Syed S, Cozart M, Haney AC, et al. Ghrelin Restoration of Function In Vitro in Somatotropes from Male Mice Lacking the Janus Kinase (JAK)-Binding Site of the Leptin Receptor. Endocrinology. 2012, 154:1565 – 1576.