october 25-26, 2017

2017 NHLBI SYSTEMS BIOLOGY SYMPOSIUM OCTOBER 25-26, 2017

LIPSETT AMPHITHEATER

NIH MEDICAL ARTS

BUILDING 10, NIH CAMPUS, BETHESDA, MD NATIONAL INSTITUTES OF HEALTH

Agenda Wednesday, October 25, 2017 8:00 a.m.

Registration Opens

1:35 p.m.

9:00 a.m.

Welcome and Director’s Remarks Keji Zhao, Ph.D. National Heart, Lung and Blood Institute

Coupling MYC Function and Expression David L. Levens, M.D., Ph.D. National Cancer Institute

2:00 p.m.

New CRISPR/Cas9 Technologies for Studies of Genetic Variation Leonid Kruglyak, Ph.D. David Geffen School of Medicine University of California, Los Angeles

2:35 p.m.

Sleep in Drosophila Melanogaster: Insights from a Systems Genetics Approach Susan T. Harbison, Ph.D. National Heart, Lung and Blood Institute

3:00 p.m.

Poster Session

Session I Chairperson: Elizabeth Murphy, Ph.D. 9:10 a.m.

9:45 a.m.

Nature’s Gift: How the Discovery of Structural Principles in a Microbial Protein Helped llluminate the Pathophysiology of Psychiatry Karl Deisseroth, M.D., Ph.D. Stanford University Howard Hughes Medical Institute The Muscle Mitochondrial Reticulum Brian Glancy, Ph.D. National Heart, Lung and Blood Institute National Institute of Arthritis and Musculoskeletal and Skin Diseases

Session III Chairperson: Mark A. Knepper, M.D., Ph.D. 4:00 p.m.

Integrated Profiling Approaches to Decipher the Metabolic Origins of Heart Disease Daniel P. Kelly, M.D. University of Pennsylvania Perelman School of Medicine

4:35 p.m.

Prolyl Hydroxylation Regulates Protein Degradation, Synthesis and Splicing in Cardiomyocytes Elizabeth Murphy, Ph.D. National Heart, Lung and Blood Institute

5:00 p.m.

Adjourn

10:10 a.m. Break/Coffee 10:30 a.m. Epigenetic Mechanisms of Tumor Initiation and Evolution Bradley E. Bernstein, M.D., Ph.D. Broad Institute of MIT and Harvard University 11:05 a.m. CTCF is a Critical Player in Controlling Gene Expression Noise Keji Zhao, Ph.D. National Heart, Lung and Blood Institute 11:30 a.m. Lunch and Poster Session Session II Chairperson: Keji Zhao, Ph.D. 1:00 p.m.

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Single-cell Epigenomics Maps the Continuous Regulatory Landscape of Human Hematopoietic Differentiation William J. Greenleaf, Ph.D. Stanford University School of Medicine

2017 NHLBI Systems Biology Symposium October 25-26, 2017

Agenda Thursday, October 26, 2017 8:00 a.m.

Registration Opens

1:20 p.m.

Metabolomic Unveils Cardiometabolic Disease Mechanisms and Therapeutic Strategies Christopher B. Newgard, Ph.D. Duke University School of Medicine

1:55 p.m.

Break and Coffee

Session IV Chairperson: Brian Gancy, Ph.D. 9:00 a.m.

The Interactome of CFTR John R. Yates III, Ph.D. The Scripps Research Institute

9:35 a.m.

A Systems Approach to the Characterization of Toll-like Receptor Response to Different Ligand Stimulation in Macrophages Alexandra Nita-Lazar, Ph.D. National Institute of Allergy and Infectious Diseases

10:00 a.m.

Break and Coffee

10:20 a.m.

Biomedical Informatics on the Cloud: A Treasure Hunt for Advancing Cardiovascular Medicine Peipei Ping, Ph.D. University of California, Los Angeles

10:55 a.m.

11:20 a.m.

Integrating NGS and Protein Mass Spectrometry Data to Discover Biological Mechanisms Mark A. Knepper, M.D., Ph.D. National Heart, Lung and Blood Institute

Session V Chairperson: Susan T. Harbison, Ph.D. 2:15 p.m.

Systems Biology of the Mitochondrion Robert S. Balaban, Ph.D. National Heart, Lung and Blood Institute

2:35 p.m.

System Level Analysis of Mutational Landscape of Cancer Teresa Przytycka, Ph.D. National Library of Medicine

3:00 p.m.

The Translational Regulation of Mitochondrial Biogenesis and Inheritance Hong Xu, Ph.D. National Heart, Lung and Blood Institute

3:25 p.m.

Closing Remarks Robert S. Balaban, Ph.D. National Heart, Lung and Blood Institute

3:30 p.m.

Adjourn

Lunch Session V Chairperson: Robert S. Balaban, Ph.D.

12:20 p.m. Peeling the Lid on Living Circuits Klaus Hahn, Ph.D. University of North Carolina School of Medicine 12:55 p.m. Understanding Transcription and Splicing Heterogeneity in Gene Expression Daniel R. Larson, Ph.D. National Cancer Institute

2017 NHLBI Systems Biology Symposium October 25-26, 2017

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Attendee Abstracts and Poster Assignments PB 1

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Georgios Amanakis Cardioprotection in mice with a knock-in mutation in Cyclophilin D (CypD-C202S): a site of S-nitrosylation Firdevs Cansu Atilgan Biochemical and Proteomic Analyses of Normal Human Astrocytes and Glioblastoma William Belden Age-related Changes in Diurnal Non-coding RNA Correlates With Changes in Genome-wide Facultative Heterochromatin Karthik R. Boppidi Impact of a Mutation in the podB Gene on Protein Productivity in Filamentous Fungi Glauber Rudá F. Braz Fluoxetine-induced Mitochondrial and Molecular Effects in the Hypothalamus of Overfed Rats Jonathon L. Burman Mitochondrial Fission Determines the Selectivity of Mitophagy Nzali Campbell Transcriptome Profiling, Variant Detection, and Pathway Analysis Link Enoyl-CoA Hydratase, Short Chain 1 to Drug Refractory Dilated Cardiomyopathy J. Harry Caufield Clinical Case Report Analysis Provides the Foundation for New Approaches to Biomedical Data Integration J. Harry Caufield Implications for Cardiac Remodeling among the Interactions of 14-3-3 Proteins Cynthia Chelius Phosphoproteomic and Transcriptomic Data Reveal New Functionality of the MAP Kinase MpkA in Aspergillus Nidulans Lihe Chen Single-cell RNA-Seq Reveals Distribution of Na, K, and Cl Transporters among the Major Cell Types of the Collecting Duct Bryan Chun Differential Regulation of Hypertrophy and Apoptosis by Beta Adrenergic Signaling in Cardiomyocytes

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Huseyin Cimen Analyses of Proteomic Changes in Prostate Cancer Upon Sodium Borate (NaB) Treatment Kairong Cui MLL4 Prepares the Enhancer Landscape for Foxp3 Induction Via Chromatin Looping Tiange Cui PreDist: A Distance-based Model as Potential Diagnostic and Prediction Classifier for Human Diseases

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Ezzat Dadkhah Comparing Three De Novo OUT Clustering Approaches, UCLUST, UPARSE, and UPGMA

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Jun Ding Interactive Dynamic Model for the Regulation of Lung Development

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2017 NHLBI Systems Biology Symposium October 25-26, 2017

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Yi Ding Dynamic Epigenetic Status and Plasticity During CD4+ T Cell Differentiation Steven Ebert Metabolomics Reveals Critical Adrenergic Regulatory Checkpoints in Glycolysis and Pentose-phosphate Pathways in Embryonic Heart Ruogu Fang STAR: Spatio-Temporal Architecture for Low-Dose Cerebral CT Perfusion Image Super-Resolution Natasha Fillmore A Role for PPARα in Sex Differences in Cardiac Hypertrophy Manasi Gadkari A Network View of the Genomic Response to Glucocorticoids in Human Primary Cells Young-Mi Go Metabolic Effects of Atherosclerosis in Disturbed Flow Mouse Model Reversed by Anti-miR205 Mark K. Halushka Does a Mosaic Pattern of Protein Expression Indicate Fast-twitch/slow-twitch Phenotypes in the Human Heart? Megan Herceg Systems Biology-driven Development of Multiplexed Biomarker Panels for the Objective Measurement of Pain Dean P. Jones Mechanistic Models for Redox Prediction of Death in Coronary Artery Disease Patients Hyun Jun Jung Gene Expression Changes in Vasopressin-Sensitive mpkCCD Cells After CRISPR/Cas9-Deletion of cAMP-Dependent Protein Kinase (PKA) Mehmet Ali Karaca Effect of Sodium Borate (NaB) Treatment on Prostate Cell Leslie Kennedy Loss of Post-Translational Prolyl Hydroxylation is Cardioprotective Via Activation of Pyruvate Dehydrogenase Arthur Ko Human Liver and Adipose Transcriptomes Reveal Potential New Cholesterol Genes and Mechanism in Fatty Liver Progression Wai Lim Ku Genome-wide Identification of Histone H2A- and Histone Variant H2A.Z-interacting Proteins by bPPI-seq Shailesh Kumar Synaptic Plasticity in PDF Expressing Circadian Neurons in Extreme Long- and Short-sleeper Inbred Lines of Drosophila Kamil C. Kural A Systems Biology Dissection of the Physiological and Accelerated Aging  Jens Lichtenberg SigSteeker and SBR-Blood: Using an Epigenetic Peak Calling Ensemble to Characterize the Red Cell System

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Julia C. Liu Molecular and Physiological Impact of Regulated Mitochondrial Calcium Uptake Lin Liu Probabilistic Modeling of Reprogramming to Induced Pluripotent Stem Cells Vivek Mahadevan Native KCC2 Interactome Reveals PACSIN1 as a Critical Regulator of Synaptic Inhibition Nathan P. Manes Multi-Omics Comparative Analysis Reveals Host Signaling Pathways Affected by the Gut Microbiota Ekaterina S. Marakasova N-acetylation of Francisella Tularensis Proteome Mark Marten Global Action of cAMP-dependent Protein Kinase (PKA) in Aspergillus Nidulans Alika K. Maunakea Delay in the Developmental Trajectory of Intragenic DNA Methylation Underlies Altered pre-mRNA Splicing in Autism Spectrum Disorders Gunjan D. Mehta Single Molecules of Transcription Factors Observed In Intracellular Space On Specific Promoters Bilal Mirza Exploring Imbalanced Machine Learning for the Analysis of Protein Dynamics in Mammalian Heart Proteome Ratnadeep Mukherjee Systematic Mapping of pSTAT Activation in Immune Cells by Single-cell Mass Cytometry

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Katy Norman New Insight into Idiopathic Pulmonary Fibrosis with Data-driven Modeling Randi J. Parks CypD-mediated Regulation of the Permeability Transition Pore is Altered in Mice Lacking the Mitochondrial Calcium Uniporter Simona Patange A Single Cell View of MYC’s Gene Regulatory and Oncogenic Mechanism Robert S. Plumb Robust Reproducible LC/MS Based Metabolomic Phenotyping of Large Cohort Epidemiological Studies

Sandra Ramey Building Resilience in an Urban Police Department Gang Ren CTCF-mediated Enhancer-promoter Interaction is a Critical Regulator of Cell-to-cell Variation of Gene Expression Ziad J. Sahab Identification of the Cellular Mechanism of Action for Xenobiotics in Less than 30 days Jian Song Integrative Method of TRIAGE Analysis Decreases Type I and Type II Error Rate in High Throughput Studies of the Macrophage Response to LPS Caetano Souto-Maior Model-based Inference from Multiple Dose, Time Course Data Reveals Infection Profiles of Type 1 Dengue Virus in Aedes Aegypti Yijun Su Lin28b Interacts with Igf2bp3 to Achieve Post-TranscriptionalRregulation of mRNA Abundance

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Junhui Sun Activation of Non-nuclear estrogen Receptor Alpha in Endothelium Reduces Cardiac Ischemia-reperfusion Injury in Mice

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Elaine Thompson Systems Level Computational Extraction of Classification Features from High-Dimensional Biological Data Sets

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Manikandan Narayanan System-wide Immune Monitoring With “Virtual” Flow Cytometry Katy Norman Inference of Cytokine Networks in Sputum and Peripheral Blood Associated with Acute Exacerbations of COPD

Mihai Podgoreanu Integrated Proteomic and Metabolomic Analysis of Myocardial Resilience to Ischemic Stress in a Hibernating Mammal

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Kedir N. Turi Integrated Analysis to Identify the Role of Newborn Metabolites in Immune-response to Acute Respiratory Syncytial Virus Infection and Association with Subsequent Childhood Recurrent Wheeze Rajanikanth Vadigepalli A Data-driven Modeling Approach to Identify Disease-specific Multi-organ Networks Driving Physiological Dysregulation

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Zhenjia Wang Functional Transcription Factor Prediction Using BART

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Junco S. Warren Multisystems Approach Reveals that Smyd1 is a Novel Regulator of Cardiac Metabolism

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Shuang Yang Chemoenzymatic Method for Analysis of Linkages of Sialylated Glycoprotein

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Angela Zeigler Predicting Drivers and Modulators of Fibroblast Phenotype During Post-Myocardial Infarction Healing

Robert S. Plumb Ion Mobility Separation of N-Glycans Directly from FFPE Colon Cancer Tissue Section in a MALDI Mass Spectrometry Imaging Experimente

2017 NHLBI Systems Biology Symposium October 25-26, 2017

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31 Center Drive MSC 2486 Bethesda, MD 20892

NHLBI Office of the Scientific Director Director

Division Intramural Research National of Heart, Lung, and Blood Institute National Heart, Lung, and Building 31, Room 5A52 Blood Institute Building Room 4-1581 31 Center10CRC, Drive MSC 2486 10 Center Drive MSC 1458 Bethesda, MD 20892 Bethesda, MD 20892

NHLBI Office of the Scientific Director

Division of Intramural Research National Heart, Lung, and Blood Institute Building 10CRC, Room 4-1581 10 Center Drive MSC 1458 Bethesda, MD 20892

NHLBI Office of the Director

National Heart, Lung, and Blood Institute Building 31, Room 5A52 31 Center Drive MSC 2486 Bethesda, MD 20892

NHLBI Office of the Scientific Director

Division of Intramural Research National Heart, Lung, and Blood Institute Building 10CRC, Room 4-1581 10 Center Drive MSC 1458 Bethesda, MD 20892