PamelaC.,Champe, Hamey R., Biochemistry , Lippincotts lllustrated Reviews |
987. 3.Peter N. Campbell , Anthony D. Smith, Illustrated Biochemistry, 2000. 4.
No.
of
BIOCHEMISTRY CODE 041,1010107 SUBIECT OUTLINE
l. hfornrtion
on the
edrcation insiurtion
Sciences and Veterinarv Medicine
of
12. Frculn
lJ. 1.4. Field
of
1.5
1.7. Form
ofeducation
2. Information on the discipline 2.1. Name of the
Chemistry and Biochemistry 2
discipline 2.2. Course coordinator 2.3. Seminar/ laboratory/ proi ect coordi nator 2.4. Year of study I 2.5. Semester
ol(f qo loto
+
Prof. Dr. Bele Constantin Prof. Dr. Bele Constantin
II
2.6. Type
of
evaluation
2.7.
Discipline
Continue
status
Content2
DF
Compulsoriness
DI
3
3. Total estimated time (teaching hours per semester) 3.1. Hours per week
- full time
DroEramme
3.4.Total number of hours in the
curriculum Distribution of the time allotted
5
70
out of which:
lecture
3.2. | : |
9:t,:l-:li"h' l+z J.).lecture |
| |
3.3. seminar/ laboratory/ ororect
2
28
l:.o.r"minar/laboratory |
hours 40
3.4.1. Study based on book, textboolq biblioeraphy and notes
3.4.2. Additional documentation in the library, specialized electronic platforms and field 3.4.3. Preparinq seminars/ laboratories/ proiects, subiects. reportsi portfolios and essays 3.4.4.Tutorials 3.4.5.Examinations 3.4.6. Other activities 3.7. Total hours of individual studv ll0 3.8. Total hours oer semester 180 3.9. Number of credits" 6
30 20 8
t2
4. Prerequisites (is applicable) 4.
l. curriculum-related
4.2. skills-related
General and Orsanic Chemistry. Analtical Chemistry . Cell Bioloev Student should possess basic knowledge about fundamental notions of chemistry , cell biolosy . anatomy . physiology and genetics.
5. Conditions (if applicable) 5.1. for the lecture 5.2. for the seminar/ laboratory/
oroiect
Interactive lecture Student should prepare for each practical
work
by reading all specific instructions.
I
6. Specific competences acquired
s-\ -!,
vt Y=
g: !)! dv
Description of structures and properties of several biomolecules (vitamins, enzymes, hormones ) involved in control and regulation of some metabolic pathways. Knowledge of the main processes specific for: -energetic metabolism. -carbohydrate metabolism. - lioid metabolism.
-Ci
Ability to work in
a team
FC
7. Course objectives (based on the list of competences acquired) 7.1. Overall course obiective
Knowledge of the structures and properties of biomolecules involved in control and regulation of metabolic pathways. Knowledge of the main metabolic pathways of carbohydrates and lipids.
7.2. Specifi c objectives
8. Content
8.1.LECTURE Number of hours
Teaching methods
-
Notes
42
I.VITAMINS. General considerations, classification I Lecture ((3hours) 3 hours
| 2lectures
and biochemical role.Hydrosoluble vitamins , correlation between vitamin and coenzyme and
biochemical imolications.Lioosoluble vitamins. 2.ENZYMES. Overview of eeneral features and I Lecture functions of enzymes in living organisms. Structure , enzyme classification and nomenclature , catalytic and allosteric sites.Kinetic of enzyme reactions. Enzyme effectors.Enzyme class description and mechanisms of reactions. 3.HORMONES. General features: structure. functions and properties. General mechanisms of hormone action. I Lecture Structure and properties of main hormones.
4.ENERGETICMETABOLISM.
lLecture
2 lectures
2 lectures
l2lectures
Biological oxidation.Citric acid cycle. Electron transport chain and oxidative phosphorylation.
S.CARBOHYDRATEMETABOLISM.-Overview. lLecture Pathways used in carbohydrate metabolism . Anaerobic glycolysis and aerobic metabolism of glucose.Pentose phosphate pathway.Glucuronic acid pathway. Gluconeogenesis.Carbohydrate metabolism disorders. 6.FAT METABOLISM. Absorption and transport lipids into blood.Catabolism of fatty acids.p- oxidation of fatty acids.Fatty acids synthesis. Synthesis and utilization of ketone bodies in animal body.Biosynthesis of cholesterol and other steroidic
of
biomolecules.Metabolism of glycerides, glycerophospholipids, sterides and sphyngolipids.Lipid metabolism disorders
8.2.
PRACTICALWORK
I Lecture
3 lectures
3 lectures
Number of hours
-
24
l.Hydrolysis of nucleoproteins and lecithines. 2.DNA separation from biological samples.
Practical works ( 2 hours )
3.Separation of proteins using magnetic nanoparticles. Size exclusion chromatography. 4. Identification of vitamins using chemical reactions. Quantification of vitamin C. 5.Extraction, chromatographic separation and
spectrophotometric quantification
of
I practical work I practical work I practical work I practical work I practical work
carotenoids from
vegetables. 6.HPLC separation and quantification of vitamin E from animal tissues. T.Enzymes properties. 8. Enzymes effectors. 9.Determination of enzymatic activity . 10. Identification of hormones usins chemical reactions.
I practical work I practical work I practical work 5 practical works
I practical work
C'ompulsory bibliognphy: I. Lehninger , A.L., Principles of Biochemistry , Fourth Editionwith CDROM, Ed. W.H. Freeman, 2004 2.PamelaC.,Champe, Hamey R., Biochemistry , Lippincotts lllustrated Reviews |987 3.Peter N. Campbell , Anthony D. Smith, Illustrated Biochemistry, 2000 4. Robert Mutay , Dovid Bender , Kathleen M. Botham , Peter J. Kennelly , Victor Rodwell , P.Anthony Llreil, Harpers lllustrated Biochemistry , 29 th Edition , LANGE Basic Science , Mcgreww-Hill Medical , 2012
Optionalbibliqraphy:
l.
C.Bele, Lipidandcubohydrate metabolism, EdituraAcafumicPres,20l0, ISBN 978-
973- 744-234.
9. Corroborating the course content with the expectations of the epistemic community representatives,
of
the professional associations and of the relevant employers in the corresponding lield The content of our discipline - afundamental science - is more or less the same with the content of biochemistry one canfind in the cuniculum of the mainfaculties of veterinary medicine in Europe.
10. Assessnent 10.3.
Type of activity
10.1. Assessment criteria
10.2. Assessment methods
Percentage of the final srade
10.4. Lecture
Written examination
Laboratory examinations
10.5. Seminar/Laboratorv 10.6.
90
o/o
r0%
Mininum oerformance standards
Filled in on 02.10.2013
Approved by the deDartment on
[8-$o^.2o1]
Course coordinator Prof. Dr. Bele Constantin
Laboratory work/seminar coordinator Prof. Dr. Bele Constantin
Head of the Department
Prof. Dr.
Aurg{ajian
// A4
a/fr /,/
