Online Coupling of Reverse-Phase and Hydrophilic Interaction. Liquid Chromatography for Protein and Glycoprotein. Characterization. Maggie P. Y. Lam, S. O. ...
Analytical and Bioanalytical Chemistry
Electronic Supplementary Material
Online Coupling of Reverse-Phase and Hydrophilic Interaction Liquid Chromatography for Protein and Glycoprotein Characterization Maggie P. Y. Lam, S. O. Siu, Edward Lau, Xiuli Mao, H. Z. Sun, Philip C. N. Chiu, William Yeung, David M. Cox, and Ivan K. Chu
Figure S1: List of proteins identified from 5 μg of mouse chrondrogenic cell lysate using RP-HILIC (≥ 95% confidence), as determined by ProteinPilot.
Figure S2: Hydrophobicity vs retention time plot of peptides in RP and HILIC. The abscissae are the retention time (min) of all peptides matched to sequences supporting protein identification (≥ 95% confidence), as determined by ProteinPilot. Retention time is here determined as the time point at which the peptide spectrum was acquired. The ordinates are the theoretical hydrophobicity of the corresponding peptides as determined by the Sequence Specific Retention Calculator using 300Å C18 material as model.
