Organism Template Amplicon Forward Primer Reverse Primer ... - PLOS

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Template pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 pJPS1232 cDNA cDNA cDNA cDNA cDNA cDNA cDNA cDNA cDNA cDNA cDNA cDNA

Amplicon RPL16 Round 1 PCR RPL16 Round 2 PCR RPL23A Round 1 PCR RPL23A Round 2 PCR RPL24A Round 1 PCR RPL24A Round 2 PCR RPL2A Round 1 PCR RPL2A Round 2 PCR RPL2B Round 1 PCR RPL2B Round 2 PCR RPL13A Round 1 PCR RPL13A Round 2 PCR RPL34A Round 1 PCR RPL34A Round 2 PCR RPL35A Round 1 PCR RPL35A Round 2 PCR RPL40B Round 1 PCR RPL40B Round 2 PCR RPSS14A Round 1 PCR RPSS14A Round 2 PCR RPS14B Round 1 PCR RPS14B Round 2 PCR RPS21B Round 1 PCR RPS21B Round 2 PCR RPS23A Round 1 PCR RPS23A Round 2 PCR RPS8A Round 1 PCR RPS8A Round 2 PCR RPS9A Round 1 PCR RPS9A Round 2 PCR RPS9B Round 1 PCR RPS9B Round 2 PCR Total RPS9A Total RPS9B SCR1 UPF1_1 UPF1_2 HNRNPLL RPLP0 RPS9 PTC+ Total exogenous RPS9 Total endogenous RPS9 GAPDH1 RPS9 cDNA ORF

Forward Primer aacaaatcgagaaaatgtctgttgaaccagttgttgtcattgatgGTAAGGGTCATTTAGTAGGTCGTTT gtaagggtcatttagtaggtcgtttagcttccgttgttgctaagcGTATTACCCTGTTATCCCTA ataatgtccggtaacggtgctcaaggtactaagtttagaatctcaTTAGGTCTACCAGTCGGTGCCATCA ttaggtctaccagtcggtgccatcatgaactgtgctgacaacagtGTATTACCCTGTTATCCCTA tgctattgccaagttaagcaagacaccaagacaataacttgagagTGATAAAATGAAGGTTGAAATCGAT tgataaaatgaaggttgaaatcgattctttttcaggtgccaaaatGTATTACCCTGTTATCCCTA ccatttctgagcaaactaagaaaccattagatcaataagcaatggGTAGAGTTATTCGTAACCAAAGAAA gtagagttattcgtaaccaaagaaagggtgctggttctatctttaGTATTACCCTGTTATCCCTA tttgtcataaactcaccaagaaaccacaaagttattgaacaatggGTAGAGTTATTCGTAACCAAAGAAA gtagagttattcgtaaccaaagaaagggtgctggttctatcttcaGTATTACCCTGTTATCCCTA tttgagaaaagcatagtacagcacagcaggaatcgtacacaatggCCATTTCCAAGAATTTACCAATCTT ccatttccaagaatttaccaatcttaaagaatcatttcagaaagcGTATTACCCTGTTATCCCTA aaccaaagatggcccaacgtgttactttcagaagaagaaatccatACAACACCCGTTCTAACAAAATCAA acaacacccgttctaacaaaatcaaggttgttaagaccccaggtgGTATTACCCTGTTATCCCTA ttattgaccgtagaataggtacagtgagacagtatattcgaaatgGCCGGTGTTAAAGCTTACGAACTAA gccggtgttaaagcttacgaactaagaaccaaatccaaggaacaaGTATTACCCTGTTATCCCTA cccgttcagcaagaggtaaagccaccaaaggttcaaaatgcaaatCTTCGTTAAGACTTTGACTGGTAAG cttcgttaagactttgactggtaagaccatcactttggaagttgaGTATTACCCTGTTATCCCTA ttaaacccaaggagagtaagaaaccacaagaacccgccatgtctaACGTTGTTCAAGCTCGTGACAATTC acgttgttcaagctcgtgacaattcccaagtttttggtgttgctaGTATTACCCTGTTATCCCTA agactgaaatcaacaactcccaataacaattaagaatggctaacgACCTTGTTCAAGCTCGCGATAACTC accttgttcaagctcgcgataactctcaagtctttggtgttgccaGTATTACCCTGTTATCCCTA aaagaatatcaataagccaaaatggaaaacgataagggtcaattaGTTGAATTATATGTTCCAAGAAAGT gttgaattatatgttccaagaaagtgttctgctaccaacagaatcGTATTACCCTGTTATCCCTA aggtttgaactctgctagaaagctacgtgtccacagaagaaacaaCCGTTGGGCCGAAAACAACTACAAG ccgttgggccgaaaacaactacaagaagagattgttgggtactgcGTATTACCCTGTTATCCCTA ttttggtactcacaagaagacgttataaacacaccaggacaaaaaTTACAAAATGGGTATTTCTCGTGAT ttacaaaatgggtatttctcgtgattctcgtcacaaaagatccgcGTATTACCCTGTTATCCCTA aaaactaatacagcaacagaaatacaaaagtatacaacatgccaaGAGCCCCAAGAACATATTCCAAGAC gagccccaagaacatattccaagacttactctaccccaaagagacGTATTACCCTGTTATCCCTA aagaaagaaagcaacaagacaactaagactaagcaacaatgccaaGAGCTCCAAGAACTTACTCTAAGAC gagctccaagaacttactctaagacttactctacaccaaagagacGTATTACCCTGTTATCCCTA ccaacatctccattcggtgg gctagaagaaacgctgctaga aggaatccgtctctctgtct GGGCGGGTgcctctcgcaaagtttgttc GGGCGGGTtggtccctacggaaactcac GGGCGGGTgatcactcggccaggaaata agaagaaggaggaggccaag tgactccgttcacaccaatc gtcgcgtcaagaggaagaac gtcgcgtcaagaggaagaac cttcttcagcgacacccatt CACCatggtgaacggccgcatac

Reverse Primer gatgctcattcaaaaaaatggatttgttagtattatcctttaaatTCCTTACCATTAAGTTGATC gatgctcattcaaaaaaatggatttgttagtattatcctttaaatTCCTTACCATTAAGTTGATC gagaataattttgttagtaaaagcatagggagaaagttagcagatTCCTTACCATTAAGTTGATC gagaataattttgttagtaaaagcatagggagaaagttagcagatTCCTTACCATTAAGTTGATC gaaaaaaaaaatgttagtaagtctatgcttttcataggtagccatTCCTTACCATTAAGTTGATC gaaaaaaaaaatgttagtaagtctatgcttttcataggtagccatTCCTTACCATTAAGTTGATC tctgttagtatacacgatacattttcaacaaaaggaataaaaagaTCCTTACCATTAAGTTGATC tctgttagtatacacgatacattttcaacaaaaggaataaaaagaTCCTTACCATTAAGTTGATC tgttagtaaatatttctcgaaagttttttcattatcaaaatatggTCCTTACCATTAAGTTGATC tgttagtaaatatttctcgaaagttttttcattatcaaaatatggTCCTTACCATTAAGTTGATC aaaaattgttagtatacagaaaagtggacgtttcaattagacaccTCCTTACCATTAAGTTGATC aaaaattgttagtatacagaaaagtggacgtttcaattagacaccTCCTTACCATTAAGTTGATC actttctcatttgaaaaatatgttagtacgaaatgtttcaataggTCCTTACCATTAAGTTGATC actttctcatttgaaaaatatgttagtacgaaatgtttcaataggTCCTTACCATTAAGTTGATC ccaaatttttattaacgttagtattttattagcacttataatgaaTCCTTACCATTAAGTTGATC ccaaatttttattaacgttagtattttattagcacttataatgaaTCCTTACCATTAAGTTGATC agtaagcacagacgtgcgtgcgtacatcataaagaaagaaacttcTCCTTACCATTAAGTTGATC agtaagcacagacgtgcgtgcgtacatcataaagaaagaaacttcTCCTTACCATTAAGTTGATC atatcaaacgtaatgttgttagtatccaaaagtttttttttttaaTCCTTACCATTAAGTTGATC atatcaaacgtaatgttgttagtatccaaaagtttttttttttaaTCCTTACCATTAAGTTGATC aacagaacttctgctttttcgattattaaaaaataaacgaatgagTCCTTACCATTAAGTTGATC aacagaacttctgctttttcgattattaaaaaataaacgaatgagTCCTTACCATTAAGTTGATC acatttgcgcttatattcttgtaaaattattctgcacatgaatgaTCCTTACCATTAAGTTGATC acatttgcgcttatattcttgtaaaattattctgcacatgaatgaTCCTTACCATTAAGTTGATC aaatgattccgttgtaaaaaacggcctcctgtgactttctgagttTCCTTACCATTAAGTTGATC aaatgattccgttgtaaaaaacggcctcctgtgactttctgagttTCCTTACCATTAAGTTGATC taaaataaagaacgtacaaaagttgcggaatttttttcttatcttTCCTTACCATTAAGTTGATC taaaataaagaacgtacaaaagttgcggaatttttttcttatcttTCCTTACCATTAAGTTGATC gtgagcgctctctttttcaaacgaatgcgaggatcaagatcactaTCCTTACCATTAAGTTGATC gtgagcgctctctttttcaaacgaatgcgaggatcaagatcactaTCCTTACCATTAAGTTGATC ttagtcaaaagttttcccggaacaaaagtctgcaagggcccaagtTCCTTACCATTAAGTTGATC ttagtcaaaagttttcccggaacaaaagtctgcaagggcccaagtTCCTTACCATTAAGTTGATC aaaatcagcacaattattcttca tgaaaaatgatccgtgatttga TGGATAAAACTCCCCTAACA GGGCGGGTgttagatcgcacgcaagaca GGGCGGGTccgagcttccttgtgttagc GGGCGGGTaccagagggatttcctccat ccgaaacccatatcatcgtc tcaagtctgcgccatgtatt cctgctagcttacgtcacca tcaagtctgcgccatgtatt accgaactcgttgtcgtacc ttagtcctcctcctcttcagc

Technique intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion intron deletion qPCR qPCR qPCR RNAi RNAi RNAi qPCR qPCR qPCR qPCR qPCR subcloning

Figure 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2A 2D 2D 2D 3A 3A 3A 3B 3B 3B 3B 3B 3B