Journal of Strength and Conditioning Research Publish Ahead of Print DOI: 10.1519/JSC.0000000000001866
ORIGINAL RESEARCH
Movement patterns and muscle damage during simulated rugby sevens matches in
D
National team players
Ramos4, Irineu Loturco1
TE
Lucas A. Pereira1, Fábio Y. Nakamura1,2, José E. Moraes3, Katia Kitamura1, Solange P.
NAR – Nucleus of High Performance in Sport, São Paulo, Brazil;
2
Department of Physical Education, State University of Londrina, Parana, Brazil;
3
Brazilian Rugby Confederation, São Paulo, Brazil;
4
Departament of Histology, State University of Londrina, Parana, Brazil.
C EP
1
C
Running head: Match running performance in rugby sevens
*Corresponding Author:
A
Lucas A. Pereira
Av. Padre José Maria, 555 - Santo Amaro, 04753-060 – São Paulo, SP, Brazil. e-mail:
[email protected]
Copyright ª 2017 National Strength and Conditioning Association
1 1 2
ABSTRACT The aim of this study was to analyze the match performance (i.e., distance covered in
4
different intensities), signs of muscle damage (assessed by means of creatine kinase [CK]
5
activity and rate of force development [RFD]), and neuromuscular fatigue (using linear sprint
6
and vertical jump performances) following three single-day simulated matches performed by
7
rugby sevens players from the Brazilian National Team. Ten male rugby sevens players (25.2
8
± 3.6 years; 88.7 ± 7.1 kg; 182.2 ± 6.3 cm) participated in this study. On the day prior to the
9
matches, the athletes performed a 40-m sprint, a vertical jump assessment and a maximal
10
isometric force test. In the morning of the match day, blood samples were collected to analyze
11
the CK activity. Afterwards, three simulated rugby sevens’ matches were performed with 2-h
12
intermission periods. The match performance (encompassing total distance and distance
13
covered in different velocity ranges and body loads [BL]) were obtained from global
14
positioning system units. The statistical analysis was performed by using a mixed model
15
approach and the effect sizes (ES) of the differences. The statistical significance level was set
16
at P< 0.05. Players were capable of maintaining the match performance when comparing the
17
first and last games. Large (ES >0.8) and significant (P< 0.05) reductions were demonstrated
18
in the total distance and BL when comparing the 2nd with the 1st halves. Decrements in the
19
explosive force capacity (assessed by means of RFD) and the squat jump were noticed (ES
TE
C EP
C
A
20
D
3
varying from 0.55 to 1.14; P< 0.05). The CK activity increased after the matches (ES = 1.29;
21
P< 0.05). The rugby sevens players were able to maintain the physical performance across
22
three successive matches simulating the first day of a tournament. The augmented CK activity
23
and the decreases in the squat jump and RFD suggest that increased levels of muscle damage
24
were experienced on the day after the matches. Therefore, the technical staff are encouraged
25
to implement recovery strategies and planned substitutions during multi-day tournaments in
Copyright ª 2017 National Strength and Conditioning Association
2 26
order to reduce the impact of accumulated fatigue and muscle damage on subsequent match
27
performance. In addition, specific training strategies aimed at better simulating the match
28
demands need to be implemented in the players’ training routines.
29 30
Keywords: team sports, ball games, recovery, performance analysis
32
D
31
INTRODUCTION
Rugby sevens took part of the Olympic Games in Rio 2016 for the first time. In the
34
rugby sevens, the rules and field dimensions are the same as in the 15-player rugby union,
35
except for the fact that the game is played by 7 players per team, in two halves with a duration
36
of 7-min each, and with a 2-min half-time interval. Remarkably, it has been shown that the
37
average velocity maintained during the rugby sevens matches is higher than in rugby union
38
(7, 26). Therefore, due to the high-intensity efforts demanded throughout these games (12, 14,
39
30), athletes are required to excel in speed, muscle power and aerobic and anaerobic
40
capacities in order to efficiently cope with the specific sport demands. In addition to physical
41
fitness development, maintenance of nutritional and hydration status throughout the
42
tournaments is fundamental for athletes to deal with rugby sevens match demands (8).
C
C EP
TE
33
Rugby sevens tournaments are generally contested over 2 consecutive days,
44
comprising 5-7 matches with ≈3-h intervals between successive games. The sequential
45
A
43
matches may lead to fatigue accumulation and reduced match performance (i.e., distance
46
covered in different intensities) across the tournament. Although only trivial to small
47
decrements in the match performance of rugby sevens players have been reported from the
48
first to the second halves (12, 30), between-match comparisons are less prevalent in the
49
literature. One study analyzing match-to-match performance variations showed unclear to
50
moderate differences in movement patterns (i.e., number of accelerations performed and
Copyright ª 2017 National Strength and Conditioning Association
3
distance covered in different intensities) between the first pool match on day one and the last
52
knock out finals match on day two in international level male players (13), while in females it
53
appears that match performance is largely reduced in similar competition settings (3).
54
However, it is currently unknown whether rugby sevens players experience physical
55
performance impairments during the first day of competition, leading to deterioration of the
56
neuromuscular/physiological traits and, consequently, hampering the athletes’ performance on
57
the second day. To date, a recent study (37) with female National and State level players
58
showed moderately attenuated vertical jump performance, a large increase in muscle soreness
59
and a very large increase in circulating creatine kinase (CK) by the end of the tournament.
60
Accordingly, decrements in countermovement jump performance related to prolonged
61
neuromuscular fatigue and muscle damage were reported for up to 7 days’ post-tournament
62
stages (37). As such, previous studies have shown that high physical demands of high-
63
intensity running, disputes and collisions acutely induce significant increases in post-game
64
circulating muscle damage markers (e.g., CK) and inflammatory mediators (e.g., neutrophils
65
and interleukin [IL]-6) immediately after two consecutive rugby sevens matches (31), and
66
between 14- and 48-h after rugby union (5) and soccer matches (28).
C EP
TE
D
51
Confirming the existence of signs of neuromuscular fatigue (i.e., reduced vertical
68
jump and sprint performances and decreases in the rate of force development [RFD]) and
69
muscle damage (i.e., increased level of CK activity) at the start of the second day of
A
70
C
67
competition in rugby sevens players would highlight the necessity of implementing nutritional
71
interventions trying to maintain glycogen levels and hydration status (8); to employ effective
72
recovery strategies at the end of day one (e.g., cold water immersion) (6); and to develop
73
more effective training strategies aiming to enhance the physical fitness components
74
necessary to allow players to better cope with the high match demands (15). Furthermore,
75
these “physical impairments” from the first to the second day could be a criterion to spare a
Copyright ª 2017 National Strength and Conditioning Association
4
player from full-match participation on the second day of competition. Therefore, the aim of
77
this study was to analyze the match performance (i.e., distance covered in different intensities
78
and body load), signs of muscle damage (i.e., CK activity and RFD), inflammatory status
79
(assessed by means of IL-6, tumor necrosis factor [TNF] α and IL-10), and neuromuscular
80
fatigue (i.e., vertical jump and sprint performances, maximum isometric force and RFD)
81
following three single-day simulated matches performed by rugby sevens players from the
82
Brazilian National Team.
D
76
84
METHODS
85
Experimental approach to the problem
TE
83
This observational study was designed to simulate the first day of a rugby sevens
87
tournament played over three consecutive matches. Figure 1 displays the schematic
88
representation of the study scheme. On the day prior to the matches (in the afternoon), the
89
athletes performed a 40-m sprint, a vertical jump assessment (squat and countermovement
90
jumps) and a maximal isometric force test in the half-squat exercise (baseline physical
91
testing). On the day of the matches, in the morning (at 8:00 am), blood samples were
92
collected (baseline measurements). Afterwards, three simulated rugby sevens matches were
93
performed with 2-h intermission periods. The match performance (encompassing total
94
distance and distance covered in different velocity ranges and body loads) were obtained from
C
A
95
C EP
86
global positioning system (GPS) units. Prior to physical testing on day 1 and day 3 and before
96
each match, a 10-min standardized warm-up involving self-selected low-intensity runs for 5
97
minutes followed by active stretching and submaximal jumps and runs was supervised by the
98
coaching staff. The matches were played in accordance with the rules of the World Rugby
99
(IRB) and under the control of an experienced referee. All the players participated in the
100
entire three matches. Finally, on the next day, the athletes returned to the testing facilities
Copyright ª 2017 National Strength and Conditioning Association
5 101
between 8:00am and 12:00pm. Blood samples were drawn and the physical tests were
102
repeated following the same order as used in the baseline measurements. All assessments
103
were performed between 15- and 17-h after the final simulated match (“post” measurements).
104
The same experienced person performed all physical assessments and the athletes were not
105
allowed to use any recovery method or substance capable of affecting the study outcomes
***INSERT FIGURE 1 HERE***
107
109
TE
108
D
106
Participants
Ten male rugby sevens players (25.2 ± 3.6 years; 88.7 ± 7.1 kg; 182.2 ± 6.3 cm)
111
from the Brazilian National team participated in the study. The players competed at
112
professional level and trained 10 to 12-h per week. Although athletes did not train in the same
113
place on a daily basis, they trained under the same training regime, supervised by the
114
coaching staff of the Brazilian National team. In addition, they took part in several training
115
camp weekends throughout the season in order to develop their technical and physical
116
abilities equally. From the 10 players, 7 comprised one team and the other 3 formed part of
117
the other team. To complete the second team, 4 players of the same competitive level
118
participated solely in the simulated matches, and did not take part in any other experimental
119
testing. From the 14 players involved in the matches, only ten were monitored due to the
C
A
120
C EP
110
availability of the GPS units. The two teams were formed by the coaching staff, matching the
121
athletes in terms of physical and technical performances. Prior to commencing the study,
122
participants were briefed about the experimental design and signed an informed consent form.
123
The study was approved by the local Ethics Committee.
124 125
Copyright ª 2017 National Strength and Conditioning Association
6 126
Match performance The game movement patterns of the rugby matches were obtained from the GPS
128
units, sampling at 5-Hz (SPI Elite, GPS ports Systems, Australia). The equipment was fitted
129
to the upper back of each player using an adjustable neoprene harness. The GPS contained a
130
tri-axial accelerometer system (sampling at 100-Hz) which was used to quantify body
131
accelerations. The units were turned on during the warm-up, to allow satellite detection, and
132
placed in the harness immediately prior to the kick-off.
D
127
The velocity ranges were selected based on a previous study (30). The match
134
activities were divided into the following categories: walking (0-6.0 km.h-1), jogging (6.1-12.0
135
km.h-1), cruising (12.1-14.0 km.h-1), striding (14.1-18.0 km.h-1), high-intensity running (18.1-
136
20.0 km.h-1) and sprinting (>20.1 km.h-1).
TE
133
The acceleration vector magnitude as a function of time (i) (AVM) was obtained
138
from x (lateral), y (frontal/back) and z (vertical) axis components (i.e., acx, acy and acz,
139
respectively) obtained from the 100 Hz accelerometer system, using the following equation:
C EP
137
140
142
C
141
Finally, the body load (BL) was calculated as the accumulated sum of all acceleration vector
144
magnitude values obtained across the matches. Since the distance covered in different
145
A
143
146
of the GPS system (5-Hz) in detecting short, but intense movements, such as
147
accelerations/decelerations and collisions, the BL has been used as an important tool for
148
match performance monitoring that quantifies all kind of movements performed by players
149
during the matches (10, 11). The BL results were divided by 100 in order to simplify the
150
expression and presentation.
intensities could underestimate the actual physical demands of the match due to the incapacity
Copyright ª 2017 National Strength and Conditioning Association
7 151 152
Neuromuscular fatigue
153
The neuromuscular fatigue was assessed by means of three different physical
154
measures: (1) the vertical jumping height, (2) the 40-m linear speed, and (3) the maximal
155
isometric force and the RFD applied in the half squat exercise. The vertical jump height was assessed through squat and countermovement jumps (SJ
157
and CMJ, respectively). The athletes performed five attempts, with a 15-s interval between
158
each jump. In the SJ, a static position with a 90° knee-flexion angle was maintained for 2-s
159
before a jump attempt without any preparatory movement. In the CMJ, athletes were
160
instructed to execute a downward movement followed by a complete extension of the legs. To
161
avoid changes in the jumping coordination pattern, the amplitude of the countermovement
162
was freely determined. All jumps were executed with the hands on the hips. The jumps were
163
performed on a contact platform (Smart Jump; Fusion Sport, Coopers Plains, Australia) with
164
the recorded flight time (t) being used to estimate the height (h) of the rise of the body’s
165
centre of gravity during the vertical jump (i.e., h = gt²/8, where g = 9.81 m·s-2). The best
166
attempt was retained for data analysis.
C EP
TE
D
156
Prior to the execution of the maximum speed test, two pairs of photocells (Smart
168
Speed®, Fusion Sport, Coopers Plains, Australia) were positioned at distances of 0- and 40-
169
m. The athletes sprinted twice, starting from a standing position, 0.5-m behind the start line. A
171
A
170
C
167
5-min rest interval was allowed between the two attempts, and the fastest time was retained for analysis.
172
Maximal isometric force and RFD in the half-squat exercise were determined using a
173
Smith-machine (Technogym Equipment, Italy), positioned over a force platform with custom
174
designed software (AccuPower, AMTI, USA), sampling at a rate of 400-Hz (36). The initial
175
position of each test was validated by an experienced test administrator, who set and fixed the
Copyright ª 2017 National Strength and Conditioning Association
8
176
bar on the safety pins at a height corresponding to ≈90° of knee flexion (to maximize the
177
force application) (8). After an initial command, subjects were instructed to push as hard and
178
fast as possible against a fixed pre-set bar, sustaining the muscle contraction for 5-s. The
179
maximal
180
achieved/collected over the force-time curve during the course of 5-s. RFD was determined as
181
the slope of the isometric force-time curve over the time-intervals of 0-100 ms (RFD100) and
182
0-200 ms (RFD200) (1, 34).Additionally, the variation in the RFD in the 100-200 ms (RFD100-
183
200)
184
marker of exercise-induced muscle damage and neuromuscular fatigue. Strong verbal
185
encouragement was provided during the tests.
force
represented
the
force
output
(peak
force)
C EP
TE
interval was calculated, since Peñailillo, et al. (24) reported that this interval is a sensitive
186 187
maximum
D
isometric
Muscle damage markers and Cytokines
Blood samples (5-ml) were collected from an antecubital arm vein into evacuated
189
tubes containing ethylenediaminetetraacetic acid (EDTA). Plasma was separated by
190
centrifugation (1,500 g, 4ºC, 10-min). Samples were collected prior to and between 15 and
191
17-h after the final match. The time for blood sample collection was determined in accordance
192
with the timetable defined by the technical staff. Plasma concentration of IL-6, TNFα, and IL-
193
10 were determined by enzyme linked immunosorbent assay (ELISA), using commercial kits
194
(Becton and Dickinson, Franklin Lakes, USA) according to the manufacturer’s instructions.
195
A
C
188
196
analyzer (Dimension EXLTM Chemistry System, Siemens, Munich, Germany). All cytokines
197
and CK analyses were performed with duplicate.
Creatine kinase activity was determined using a commercial kit in an automated biochemical
198 199 200
Copyright ª 2017 National Strength and Conditioning Association
9 201
Statistical analyses The Shapiro-Wilk test was used to check the data normality. To compare the CK
203
activity and cytokines and the performance in the physical tests (at baseline and after the
204
simulated matches), a paired t-test was used. A linear mixed model analysis was used to
205
compare the distance covered in different ranges of velocities and also to compare the body
206
load accumulated between match halves (during each of the three matches and between-
207
matches). This approach is used in general linear models with repeated measures and has the
208
ability to compare the slope among curves (i.e., rate of change in the distance covered across
209
the matches) (35). Accordingly, two factors were included for analyses: match number and
210
period (i.e., first and second halves). The Tukey’s post-hoc test identified where the possible
211
differences occurred. The level of statistical significance was set at P< 0.05. Finally, the
212
magnitudes of the differences for the comparisons in all variables were analyzed using the
213
Cohen’s d effect size (ES) and its confidence intervals (CI) (4).The magnitudes of the ESs
214
were qualitatively interpreted using the following thresholds: 0.8, large (4).
C EP
TE
D
202
216
RESULTS
C
217
Table 1 demonstrates the comparisons of the total distance and the distance covered in
219
the different velocity ranges among the three consecutive matches. The distance covered in
220
A
218
the 0-6 km.h-1 and 6-12 km.h-1 velocity ranges in match 3 was significantly shorter than in
221
matches 1 and 2, respectively (16.2% and 9.2% of difference; P< 0.05). During match 2,
222
athletes covered greater distances in the 6-12 km.h-1 and 12-14 km.h-1 velocity ranges in
223
relation to match 1 (15.8% and 19.2% of difference, respectively; P< 0.05), while covering
224
shorter distance in sprinting (>20 km.h-1) than during match 1 (50.5% of difference; P< 0.05).
Copyright ª 2017 National Strength and Conditioning Association
10 225
No differences in BL were observed comparing the three matches (match 1: 977.8 ± 26.8 a.u.;
226
match 2: 975.6 ± 27.3 a.u.; match 3: 973.4 ± 18.6 a.u., P> 0.05).
227
***INSERT TABLE 1 HERE***
228 229
Figure 2 shows the comparisons between the 1st and 2nd halves for total distance and
231
distance covered in the different velocity ranges. The athletes covered a shorter total distance
232
and shorter distances in jogging (6-12.0 km.h-1), cruising (12.0-14.0 km.h-1), and striding
233
(14.0-18.0 km.h-1) in the 2nd half compared to the 1st half (ES [90% CI] % of difference: -1.30
234
[-2.02; -0.42] 9.6%, -1.33 [-1.86; -0.29] 20.7%, -1.52 [-2.40; -0.72] 31.8%, -0.92 [-1.65; -
235
0.11] 21.4%, respectively; P< 0.05). The distance covered walking (0-6 km.h-1) was
236
significantly greater in the 2nd half than in the 1st half (ES: 1.72 [0.69; 2.36] 19.1%; P< 0.05).
237
No difference was observed in the distance covered at high-intensity (18.0-20.0 km.h-1) and
238
sprinting (>20 km.h-1) between the two halves (ES: -0.43 [-1.20; 0.29], -0.04 [-0.77; 0.70],
239
respectively; P> 0.05). Figure 3 shows the comparison of the BL between the 1st and 2nd
240
halves. The 2nd half presented a significantly lower BL compared to the 1st half (ES: -1.28 [-
241
2.00; -0.40] 3.5%; P< 0.05).
244 245
TE
C EP
C
243
A
242
D
230
***INSERT FIGURE 2 HERE***
***INSERT FIGURE 3 HERE***
246 247
Table 2 shows the comparisons of the physical test results at baseline and following
248
the three consecutive rugby sevens matches. The SJ and RFD measured in 0-100 ms, 0-200
249
ms, 100-200 ms were significantly lower in the post- than in the baseline-tests (5.7%, 28.8%,
Copyright ª 2017 National Strength and Conditioning Association
11 250
26.7%, and 23.1% of difference, respectively; P< 0.05). No significant differences were
251
observed in the CMJ, 40-m sprinting speed, or maximum isometric force comparing the
252
baseline with the post-assessments. Table 3 displays the serum CK activity and cytokine concentration at baseline and 15-
254
h after the final match. The CK activity was significantly higher in the post- than in the
255
baseline-measurements (83.2% of difference; P< 0.05). No significant differences were
256
observed in the cytokine concentration comparing the two moments (i.e., baseline vs post-
257
measurements; P> 0.05).
TE
D
253
258
***INSERT TABLE 2 HERE***
259 260
C EP
***INSERT TABLE 3 HERE***
261 262 263
DISCUSSION
The main finding of this study was that the investigated players were capable of
265
maintaining the activity profile and intensity of the match, between the first and last matches
266
on the first day of a simulated rugby sevens tournament. Significant reductions with a large
267
effect size were demonstrated in the total distance and BL when comparing the 2nd with the 1st
268
half of all games combined. In the physical tests (performed 15-h after the matches), there
A
269
C
264
were decrements in the SJ height and in the RFD (assessed in 0-100, 0-200, and 100-200 ms)
270
obtained from the half-squat maximal isometric force test. The CK activity increased 15-h
271
after the matches, whereas inflammatory cytokines did not substantially change from
272
baseline. These results suggest that the rugby sevens players experienced substantial levels of
273
muscle damage and neuromuscular fatigue (i.e., increased CK activity and reduced RFD)
Copyright ª 2017 National Strength and Conditioning Association
12 274
which was accompanied by decreased performance in the vertical jump height 15-h after three
275
simulated rugby sevens matches. The total distance (1413.3-m per match on average) and the distance covered at high-
277
intensity (80.1-m per match on average) and sprinting (146.7-m per match on average) during
278
matches reported herein are strictly in line with previous studies that analyzed national and
279
international level rugby sevens players (13, 27, 30). For instance, Suarez-Arrones, et al. (30)
280
demonstrated that during national level matches, these athletes might cover a total of 1500-m,
281
being 79.5-m at high-intensity efforts and 137.7-m at maximal sprinting speed. Furthermore,
282
although using a slightly different threshold for detecting sprinting (i.e., 21.6 km.h-1), a
283
previous study (13) demonstrated higher total distance (120 m.min-1) and similar distance in
284
sprinting (11.5 m.min-1) during domestic and international matches in comparison to those
285
observed in our study (101 m.min-1 and ≈10.5 m.min-1, respectively). Meanwhile, Ross, et al.
286
(27) revealed that sevens players could cover a total of 105 m.min-1, 9 m.min-1 being in
287
sprinting, during provincial and/or international matches. Together, these data suggest that the
288
intensity of the sevens matches investigated in the present study is comparable with those
289
already observed during top-level matches (13, 27, 30).
C EP
TE
D
276
A previous study analyzing the profile of players’ activities over a rugby sevens
291
tournament showed only small reductions in the distance covered in low-intensity running
292
(90% maximal
303
heart rate increased in the second half (in comparison with the first half). Moreover, when
304
compared with athletes who participated in the entire match, the substitute players covered
305
greater distances while running at high intensities during the 2nd half (13). From an applied
306
standpoint, these results indicate that, despite the absence of signs of permanent fatigue
307
throughout successive matches, temporary fatigue can be observed during each match.
308
Although the mechanisms underlying fatigue experienced during the matches were not fully
309
elucidated, some studies have already suggested that this could be caused by metabolic and
310
neural disturbances, such as ionic imbalances and central fatigue (2, 20, 33). It is important to
311
note that we did not permit substitutions of the monitored players during the three matches; as
312
aforementioned, substitute players may cover higher distances when compared with players
313
involved in the entire match, which could compromise the objectives of our investigation (14,
314
26).
C
C EP
TE
D
298
The increased blood CK activity suggests that substantial level of muscle damage
316
may occur after one-day of simulated competition. For instance, Takahashi, et al. (31)
317
A
315
demonstrated an increase of 42% in CK after two consecutive sevens matches. In the present
318
study, we showed an increase of 83% in the CK activity 15-h after three consecutive matches.
319
In spite of the large increase in the CK activity reported herein, this increase is lower than
320
those demonstrated by West, et al. (37) (250% and 500% of increase after the 1st and 2nd day
321
of competition, respectively) and by Clarke, et al. (3) (126% of increase) after official
322
tournaments, in male and female sevens players, respectively. Possibly, the higher CK values
Copyright ª 2017 National Strength and Conditioning Association
14
demonstrated by Clarke, et al. (3) are related to the fact that the athletes covered greater
324
distances in sprinting than our players (13.5 m.min-1 vs. 10.5 m.min-1) (23). Additionally,
325
these studies were performed during official tournaments, which probably induced higher
326
physical engagement in specific playing actions (e.g., collisions), consequently entailing
327
greater signs of muscle damage (23, 29, 32). In this regard, it is essential to further examine
328
the physical/physiological differences between “friendly” and competitive rugby sevens
329
matches.
D
323
There was a moderate decrease of 5.7% in the SJ height, 4.8% in the CMJ height
331
(non-significant) and a large decrease of 23% in the RFD100-200. This reduction in vertical
332
jumping performance (SJ and CMJ) is in accordance with previous studies that demonstrated
333
neuromuscular fatigue (i.e., measured by means of jump height) after professional rugby
334
union (38), rugby league (21), and consecutive rugby sevens matches (37). Importantly, a
335
previous study indicated that reductions in the RFD100-200 were highly associated with
336
exercise-induce muscle damage and neuromuscular fatigue (24). Indeed, it has been shown
337
that decreases in SJ height, CMJ height, and RFD100-200 (in association with increased levels
338
of CK) might be related to significant impairments in the contractile machinery (24). From a
339
physiological perspective, the decrement in the capacity to quickly apply/produce force and
340
the symptoms associated with muscle damage and fatigue may be connected to the repetitive
341
involvements in high-intensity efforts, such as collisions and maximum sprints during the
C EP
C
A
342
TE
330
matches (21, 22). Although the SJ and CMJ height have been shown to be highly associated
343
with sprinting speed (16, 17), at least for this group of top-level rugby sevens players, the
344
reductions in the vertical jumping performance were not accompanied by decreases in the
345
maximal sprinting capacity. This suggests that the extent of muscle damage and fatigue
346
induced in our sample was not able to affect/disturb the speed performance. On the other
347
hand, the signs of muscle damage described here might compromise the ability of the players
Copyright ª 2017 National Strength and Conditioning Association
15 348
to perform maximally during the second day of competition, strongly indicating the necessity
349
of implementing effective recovery methods during the transition from the first to the second
350
day of competition during rugby sevens tournaments (6). This study presented some inherent limitations, listed as follows: 1) although players
352
received similar meals and could ingest water ad libitum during the study duration, the exact
353
composition of the food portions and the hydration status were not controlled; 2) the baseline
354
physical tests on day 1 were performed in the afternoon, while the post-match tests on day 3
355
were performed in the morning; 3) since circadian variations are expected in some physical
356
performance indices (9, 25), our results could have been somewhat biased by this biological
357
factor.
TE
D
351
In summary, the rugby sevens players were able to maintain the game movement
359
patterns across three successive matches simulating the first day of a tournament. In addition,
360
the increased levels of CK and the decreases in the SJ and RFD100-200 suggest that substantial
361
levels of muscle damage and neuromuscular fatigue occurred in the day after the matches.
362
This probably causes significant physical performance impairments on the second day of
363
competition. Finally, additional studies are needed to comprehensively examine the efficacy
364
of different recovery methods for restoring the physical and physiological traits of these elite
365
athletes between successive matches or training sessions. Certainly, these applied approaches
366
will positively influence the competitive performance of these players throughout the rugby
C
A
367
C EP
358
sevens’ tournaments.
368 369 370 371 372
Copyright ª 2017 National Strength and Conditioning Association
16 373
PRACTICAL APPLICATIONS Sevens’ tournaments are typically executed over two consecutive days, with players
375
performing up to seven matches, interspersed with short recovery intervals (i.e., ≈3-4 h). After
376
examining the data collected in this study, it was revealed that these athletes could effectively
377
maintain the game movement patterns during three-successive sevens matches. However, on
378
the second day, they experienced meaningful signs of muscle damage and a compromised
379
capacity to produce explosive force (i.e., RFD). In this sense, coaches and scientists involved
380
in this sport are strongly encouraged to implement and create specific strategies able to
381
improve (and reduce the impairments) in the neuromuscular function of their athletes.
382
Intrinsically, training components such as plyometric and eccentric exercises may be optimal
383
alternatives to increase the “explosive muscle strength” (18) and enhance the protective effect
384
against muscle damage (19) in sevens players.
386
REFERENCES
387
1.
C EP
385
TE
D
374
Aagaard P, Simonsen EB, Andersen JL, Magnusson P, and Dyhre-Poulsen P. Increased rate of force development and neural drive of human skeletal muscle
389
following resistance training. J Appl Physiol 93: 1318-1326, 2002.
391 392
2.
Aughey RJ. Australian football player work rate: evidence of fatigue and pacing? Int J Sports Physiol Perform 5: 394-405, 2010.
A
390
C
388
3.
Clarke AC, Anson JM, and Pyne DB. Neuromuscular Fatigue and Muscle Damage
393
After a Women's Rugby Sevens Tournament. Int J Sports Physiol Perform 10: 808-
394
814, 2015.
395 396
4.
Cohen J. Statistical power analysis for the behavioral sciences. Hillsdale (NJ): Lawrence Erlbaum Associates, 1988.
Copyright ª 2017 National Strength and Conditioning Association
17 397
5.
Cunniffe B, Hore AJ, Whitcombe DM, Jones KP, Baker JS, and Davies B. Time
398
course of changes in immuneoendocrine markers following an international rugby
399
game. Eur J Appl Physiol 108: 113-122, 2010.
400
6.
Douglas J, Plews DJ, Handcock PJ, and Rehrer NJ. The Beneficial Effect of Parasympathetic Reactivation on Sympathetic Drive During Simulated Rugby Sevens.
402
Int J Sports Physiol Perform 11: 480-488, 2016. 7.
union. Sports Med 33: 973-991, 2003.
404 405
8.
Dziedzic CE and Higham DG. Performance nutrition guidelines for international rugby sevens tournaments. Int J Sport Nutr Exerc Metab 24: 305-314, 2014.
406 407
Duthie G, Pyne D, and Hooper S. Applied physiology and game analysis of rugby
TE
403
D
401
9.
Edwards BJ, Pullinger SA, Kerry JW, Robinson WR, Reilly TP, Robertson CM, and Waterhouse JM. Does raising morning rectal temperature to evening levels offset the
409
diurnal variation in muscle force production? Chronobiol Int 30: 486-501, 2013.
410
10.
Ehrmann FE, Duncan CS, Sindhusake D, Franzsen WN, and Greene DA. GPS and injury prevention in professional soccer. J Strength Cond Res 30: 360-367, 2016.
411 412
C EP
408
11.
Gomez-Piriz PT, Jimenez-Reyes P, and Ruiz-Ruiz C. Relation between total body load and session rating of perceived exertion in professional soccer players. J Strength
414
Cond Res 25: 2100-2103, 2011.
416
12.
BM. Match analysis and temporal patterns of fatigue in rugby sevens. J Strength Cond Res 28: 728-734, 2014.
417 418
Granatelli G, Gabbett TJ, Briotti G, Padulo J, Buglione A, D'Ottavio S, and Ruscello
A
415
C
413
13.
Higham DG, Pyne DB, Anson JM, and Eddy A. Movement patterns in rugby sevens:
419
effects of tournament level, fatigue and substitute players. J Sci Med Sport 15: 277-
420
282, 2012.
Copyright ª 2017 National Strength and Conditioning Association
18 421
14.
Higham DG, Pyne DB, Anson JM, and Eddy A. Physiological, anthropometric, and
422
performance characteristics of rugby sevens players. Int J Sports Physiol Perform 8:
423
19-27, 2013.
424
15.
Higham DG, Pyne DB, Anson JM, Hopkins WG, and Eddy A. Comparison of activity profiles and physiological demands between international rugby sevens matches and
426
training. J Strength Cond Res 30: 1287-1294, 2016.
427
16.
D
425
Loturco I, D'Angelo RA, Fernandes V, Gil S, Kobal R, Abad CCC, Kitamura K, and Nakamura FY. Relationship between sprint ability and loaded/unloaded jump tests in
429
elite sprinters. J Strength Cond Res 29: 758-764, 2015.
430
17.
TE
428
Loturco I, Pereira LA, Cal Abad CC, D'Angelo RA, Fernandes V, Kitamura K, Kobal R, and Nakamura FY. Vertical and horizontal jump tests are strongly associated with
432
competitive performance in 100-m dash events. J Strength Cond Res 29: 1966-1971,
433
2015.
434
18.
Markovic G and Mikulic P. Neuro-musculoskeletal and performance adaptations to lower-extremity plyometric training. Sports Med 40: 859-895, 2010.
435 436
C EP
431
19.
McHugh MP. Recent advances in the understanding of the repeated bout effect: the protective effect against muscle damage from a single bout of eccentric exercise.
438
Scand J Med Sci Sports 13: 88-97, 2003.
440
20.
Na+-K+ pump inactivation: implications for fatigue. J Appl Physiol (1985) 104: 288295, 2008.
441 442
21.
445
McLellan CP and Lovell DI. Neuromuscular responses to impact and collision during elite rugby league match play. J Strength Cond Res 26: 1431-1440, 2012.
443 444
McKenna MJ, Bangsbo J, and Renaud JM. Muscle K+, Na+, and Cl disturbances and
A
439
C
437
22.
McLellan CP, Lovell DI, and Gass GC. Markers of postmatch fatigue in professional Rugby League players. J Strength Cond Res 25: 1030-1039, 2011.
Copyright ª 2017 National Strength and Conditioning Association
19 446
23.
Oxendale CL, Twist C, Daniels M, and Highton J. The Relationship Between Match-
447
Play Characteristics of Elite Rugby League and Indirect Markers of Muscle Damage.
448
Int J Sports Physiol Perform 11: 515-521, 2016. 24.
as a measure of muscle damage. Scand J Med Sci Sports 25: 417-427, 2015. 25.
output and repeated-sprint ability. Chronobiol Int 22: 1029-1039, 2005.
452 453
26.
Ross A, Gill N, and Cronin J. Match analysis and player characteristics in rugby sevens. Sports Med 44: 357-367, 2014.
454 455
Racinais S, Connes P, Bishop D, Blonc S, and Hue O. Morning versus evening power
D
450 451
Peñailillo L, Blazevich AJ, Numazawa H, and Nosaka K. Rate of force development
27.
TE
449
Ross A, Gill ND, and Cronin JB. A Comparison of the Match Demands of International and Provincial Rugby Sevens. Int J Sports Physiol Perform 10: 786-790,
457
2015.
458
28.
C EP
456
Silva JR, Ascensao A, Marques F, Seabra A, Rebelo A, and Magalhaes J.
459
Neuromuscular function, hormonal and redox status and muscle damage of
460
professional soccer players after a high-level competitive match. Eur J Appl Physiol
461
113: 2193-2201, 2013. 29.
Smart DJ, Gill ND, Beaven CM, Cook CJ, and Blazevich AJ. The relationship
C
462
between changes in interstitial creatine kinase and game-related impacts in rugby
464
union. Br J Sports Med 42: 198-201, 2008.
465
A
463
30.
Suarez-Arrones LJ, Nunez FJ, Portillo J, and Mendez-Villanueva A. Running
466
demands and heart rate responses in men Rugby Sevens. J Strength Cond Res 26:
467
3155-3159, 2012.
468
31.
Takahashi I, Umeda T, Mashiko T, Chinda D, Oyama T, Sugawara K, and Nakaji S.
469
Effects of rugby sevens matches on human neutrophil-related non-specific immunity.
470
Br J Sports Med 41: 13-18, 2007.
Copyright ª 2017 National Strength and Conditioning Association
20
32.
to tackle plays. Br J Sports Med 37: 416-419, 2003.
472 473
33.
34.
Thorlund JB, Aagaard P, and Madsen K. Rapid muscle force capacity changes after soccer match play. Int J Sports Med 30: 273-278, 2009.
476 477
Taylor JL and Gandevia SC. A comparison of central aspects of fatigue in submaximal and maximal voluntary contractions. J Appl Physiol (1985) 104: 542-550, 2008.
474 475
Takarada Y. Evaluation of muscle damage after a rugby match with special reference
35.
D
471
Ugrinowitsch C, Fellingham GW, and Ricard MD. Limitations of ordinary least squares models in analyzing repeated measures data. Med Sci Sports Exerc 36: 2144-
479
2148, 2004.
480
36.
TE
478
Walsh MS, Ford KR, Bangen KJ, Myer GD, and Hewett TE. The validation of a portable force plate for measuring force-time data during jumping and landing tasks. J
482
Strength Cond Res 20: 730-734, 2006.
483
37.
C EP
481
West DJ, Cook CJ, Stokes KA, Atkinson P, Drawer S, Bracken RM, and Kilduff LP.
484
Profiling the time-course changes in neuromuscular function and muscle damage over
485
two consecutive tournament stages in elite rugby sevens players. J Sci Med Sport 17:
486
688-692, 2014. 38.
West DJ, Finn CV, Cunningham DJ, Shearer DA, Jones MR, Harrington BJ, Crewther
C
487
BT, Cook CJ, and Kilduff LP. Neuromuscular function, hormonal, and mood
489
responses to a professional rugby union match. J Strength Cond Res 28: 194-200,
490
A
488
2014.
491 492 493 494 495
Copyright ª 2017 National Strength and Conditioning Association
21 496
FIGURE LEGENDS
497 498
Figure 1. Schematic illustration of the study design.
499
Figure 2. Comparisons between the 1st and 2nd halves for total distance covered and distance
501
covered in the different velocity ranges during the rugby sevens matches.
D
500
502
Figure 3. Comparison of the body load between the 1st and 2nd halves during the rugby sevens
504
matches.
A
C
C EP
TE
503
Copyright ª 2017 National Strength and Conditioning Association
Table 1. Total distance (TD) and distance covered in different velocity ranges in the three rugby sevens matches.
Ranges
Match 1
Match 2
Match 3
M1 vs. M3
M2 vs. M3
ES (90% CI) - rating ES (90% CI) - rating ES (90% CI) - rating 470.1 ± 34.4
443.8 ± 32.2
393.9 ± 31.0*
6-12 km/h (m)
358.6 ± 43.8
415.3 ± 62.5*
325.6 ± 58.2#
12-14 km/h (m)
128.8 ± 16.0
153.5 ± 20.1*
143.2 ± 33.1
14-18 km/h (m)
234.9 ± 57.1
245.9 ± 58.6
18-20 km/h (m)
78.1 ± 25.6
69.3 ± 22.9
> 20 km/h (m)
182.5 ± 89.6
90.4 ± 36.8*
1453.2 ± 120.8
1417.0 ± 88.2
248.7 ± 45.7
93.0 ± 27.4
167.1 ± 59.6
C
A
-0.76 (-1.52; 0.01)
-2.22 (-3.17; -1.29)
-1.55 (-2.35; -0.68)
Moderate
Large
Large
1.29 (0.23; 1.79)
-0.75 (-1.37; 0.14)
-1.44 (-2.25; -0.60)
Large
Moderate
Large
1.54 (0.49; 2.11)
0.90 (-0.22; 1.28)
-0.51 (-1.10; 0.38)
Large
Moderate
Moderate
0.19 (-0.56; 0.92)
0.24 (-0.48; 0.99)
0.05 (-0.68; 0.79)
Trivial
Small
Trivial
-0.34 (-1.09; 0.39)
0.58 (-0.21; 1.29)
1.03 (0.13; 1.67)
Small
Moderate
Moderate
-1.03 (-2.21; -0.66)
-0.17 (-0.93; 0.54)
2.08 (0.65; 3.51)
Large
Trivial
Large
-0.30 (-1.07; 0.41)
-0.69 (-1.54; -0.01)
-0.53 (-1.28; 0.22)
Small
Moderate
Moderate
C EP
0-6 km/h (m)
TD (m)
M1 vs. M2
TE D
Velocity
1370.3 ± 79.9
Note - CI: confidence interval; ES: effect size; *different from match 1, P< 0.05; #different from match 2, P< 0.05.
Copyright ª 2017 National Strength and Conditioning Association
Table 2. Comparison of the physical tests at baseline (pre) and after (post) three consecutive rugby sevens matches. Post
ES (90% CI) - rating
SJ (cm)
42.0 ± 4.4
39.6 ± 3.3*
-0.55 (-1.34; 0.16) Moderate
CMJ (cm)
41.1 ± 3.8
39.1 ± 3.4
-0.53 (-1.28; 0.22) Moderate
Sprinting 40 m (s)
5.0 ± 0.2
5.0 ± 0.2
0.14 (-0.60; 0.88) Trivial
RFD100 (N.ms-1)
730.0 ± 255.6
519.6 ± 180.1*
-0.82 (-1.68; -0.14) Large
RFD200 (N.ms-1)
1171.8 ± 345.4
859.3 ± 266.2*
-0.90 (-1.75; -0.19) Large
441.8 ± 89.8
339.7 ± 86.1*
-1.14 (-1.90; -0.32) Large
2949.3 ± 328.4
2821.6 ± 388.0
-0.39 (-1.08; 0.40) Small
RFD100-200 (N.ms-1) MIF (N)
TE D
Pre
Note - SJ: squat jump; CMJ: countermovement jump; RFD: rate of force development in 100
A C
C EP
ms, 200 ms, and 100-200 ms; MIF: maximum isometric force; *P< 0.05.
Copyright ª 2017 National Strength and Conditioning Association
Table 3. Serum creatine kinase (CK) activity and cytokine concentration at baseline (pre) and 15-h post three consecutive rugby sevens matches. Pre
Post
ES (90% CI) - rating
CK (Units.l-1)
699.6 ± 451.0
1282.0 ± 532.2*
1.29 (0.34; 1.92) Large
IL-6 (pg.ml-1)
1.68 ± 0.86
1.59 ± 0.84
-0.10 (-0.84; 0.63) Trivial
TNFα (pg.ml-1)
1.14 ± 0.62
1.31 ± 0.57
0.27 (-0.47; 1.01) Small
4.79 ± 2.07
4.80 ± 2.14
0.01 (-0.73; 0.74) Trivial
-1
D
IL-10 (pg.ml )
A
C
C EP
TE
Note - IL-6: interleukin-6; IL-10: interleukin-10; TNFα: tumor necrosis factor - α; *P< 0.05.
Copyright ª 2017 National Strength and Conditioning Association
D TE C EP C A Copyright ª 2017 National Strength and Conditioning Association
D TE C EP C A Copyright ª 2017 National Strength and Conditioning Association
D TE C EP C A Copyright ª 2017 National Strength and Conditioning Association