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OVERLOAD STIMULATION AT THE ONSET OF TYPE I DIABETES PRESERVES THE SOLEUS BUT NOT THE EDL MUSCLE MASS IN WISTAR RATS
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Marco Aurélio Salomão Fortes1,*; Maria Vitória Martins Scervino1; Gabriel Nasri Marzuca-Nassr1,2; Kaio Fernando Vitzel3; Carlos Hermano da Justa Pinheiro1 and Rui Curi1,4
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1 Department of Physiology and Biophysics, Institute of Biomedical Sciences,
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University of São Paulo, Sao Paulo, Brazil
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2 Department of Internal Medicine, Faculty of Medicine, Universidad de La Frontera,
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Temuco, Chile.
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3 Massey Institute of Food Science and Technology, College of Health, Massey
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University, Albany, New Zealand
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4 Interdisciplinary Post-Graduate Program in Health Sciences, Cruzeiro do Sul
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University, Sao Paulo, Brazil
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*Corresponding author: Marco Aurélio Salomão Fortes, Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, room 105, 1524 Prof. Lineu Prestes Av, Cidade Universitária, Butantã, São Paulo, SP - CEP:05508-000, Brazil. Tel: +55 11 3091 7245. E-mail:
[email protected]
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Supplementary Figures and Tables
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Supplementary Figure 1: Ponceau and gels obtained by western blotting of the total Akt protein and its phosphorylated form in the EDL muscle after 7 days of overload. 5 to 7 animals were used for the plot. DMCL – contralateral muscle diabetic group; DMH – hypertrophied muscle diabetic group; CL contralateral; H – hypertrophy; P - pool containing a mixture with equal parts of all samples – used to normalize Ponceau S quantitative results.
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Supplementary Figure 2: Ponceau and gels obtained by western blotting of the total rpS6 protein and its phosphorylated form in the EDL muscle after 7 days of overload. 5 to 7 animals were used for the plot. DMCL – contralateral muscle of the diabetic group; DMH – hypertrophied muscle of the diabetic group;
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CL - contralateral; H – hypertrophy; P - pool containing a mixture with equal parts of all samples – used to normalize Ponceau S quantitative results.
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Supplementary Figure 3: Ponceau and gels obtained by western blotting of the total 4EBP-1 protein and its phosphorylated form in the EDL muscle after 7 days of overload. 5 to 7 animals were used for the plot. DMCL – contralateral muscle of the diabetic group; DMH – hypertrophied muscle of the diabetic group; CL - contralateral; H – hypertrophy; P - pool containing a mixture with equal parts of all samples – used to normalize Ponceau S quantitative results.
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Supplementary Figure 4: Ponceau and gels obtained by western blotting of the total AMPKα1 protein and its phosphorylated form in the EDL muscle after 7 days of overload. 5 to 6 animals were used for the plot. DMCL – contralateral muscle of the diabetic group; DMH – hypertrophied muscle of the diabetic
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group; CL - contralateral; H – hypertrophy; P - pool containing a mixture with equal parts of all samples – used to normalize Ponceau S quantitative results.
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Supplementary Figure 5: Ponceau and gels obtained by western blotting of the MuRF-1 and atrogin-1 in the EDL muscle after 7 days of overload. 7 to 8 animals were used for the plot. DMCL – contralateral muscle of the diabetic group; DMH – hypertrophied muscle of the diabetic group; CL - contralateral; H – hypertrophy; P - pool containing a mixture with equal parts of all samples – used to normalize Ponceau S quantitative results.
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Supplementary Figure 6: Ponceau and gels obtained by western blotting of the total Akt protein and its phosphorylated form in the soleus muscle after 7 days of overload. 6 animals were used for the plot.
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DMCL – contralateral muscle diabetic group; DMH – hypertrophied muscle diabetic group; CL contralateral; H – hypertrophy; P - pool containing a mixture with equal parts of all samples – used to normalize Ponceau S quantitative results.
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Supplementary Figure 7: Ponceau and gels obtained by western blotting of the total rpS6 protein and its phosphorylated form in the soleus muscle after 7 days of overload. 6 animals were used for the plot. DMCL – contralateral muscle of the diabetic group; DMH – hypertrophied muscle of the diabetic group; CL - contralateral; H – hypertrophy; P - pool containing a mixture with equal parts of all samples – used to normalize Ponceau S quantitative results.
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Supplementary Figure 8: Ponceau and gels obtained by western blotting of the total 4EBP-1 protein and its phosphorylated form in the soleus muscle after 7 days of overload. 6 animals were used for the plot. DMCL – contralateral muscle of the diabetic group; DMH – hypertrophied muscle of the diabetic group; CL - contralateral; H – hypertrophy; P - pool containing a mixture with equal parts of all samples – used to normalize Ponceau S quantitative results.
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Supplementary Figure 9: Ponceau and gels obtained by western blotting of the total AMPKα1 protein and its phosphorylated form in the soleus muscle after 7 days of overload. 6 animals were used for the plot. DMCL – contralateral muscle of the diabetic group; DMH – hypertrophied muscle of the diabetic group; CL - contralateral; H – hypertrophy; P - pool containing a mixture with equal parts of all samples – used to normalize Ponceau S quantitative results.
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Supplementary Figure 10: Ponceau and gels obtained by western blotting of the MuRF-1 and atrogin-1 in the soleus muscle after 7 days of overload. 6 animals were used for the plot. DMCL – contralateral muscle of the diabetic group; DMH – hypertrophied muscle of the diabetic group; CL - contralateral; H – hypertrophy; P - pool containing a mixture with equal parts of all samples – used to normalize Ponceau S quantitative results.
