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Journals A-Z > Plastic & Reconstructive ... > 130(2) August 2012 > LOP06: In Vivo ... Article Tools
Plastic and Reconstructive Surgery Issue: Volume 130(2S) EPSRC 2012 Abstracts Supplement, August 2012, p 480
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Copyright: ©2012American Society of Plastic Surgeons Publication Type: [European PSRC Abstract Supplement: Abstracts]
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DOI: 10.1097/01.prs.0000418398.33827.30 ISSN: 0032-1052
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Accession: 00006534-201208001-00008
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LOP06: In Vivo Sensory Recordings Using a Novel Skin-Flap Chamber and Custom Mechanical Indenter Sugg, K.1; Urbanchek, M.1; Baba, Y.2; Kim, E.3; Lumpkin, E.2; Gerling, G.3; Cederna, P.1; Langhals, N.1
Author Information 1
University of Michigan, Plastic and Reconstructive Surgery, Ann Arbor, USA
2
Columbia University College of Physicians & Surgeons, Dermatology, Physiology, and Cellular Biophysics, New York, USA
3
University of Virginia, Systems and Information Engineering, Charlottesville, USA
Papers are reprinted as they were submitted. The EPSRC takes no responsibility for typographical or other errors.
Find Citing Articles About this Journal Request Permissions Outline INTRODUCTION: MATERIALS AND METHODS: RESULTS: CONCLUSIONS: ACKNOWLEDGEMENTS:
INTRODUCTION: Touch receptors are responsible for converting force into an electrical signal. After a mechanical stimulus is applied to the skin surface, it becomes encoded as a unique train of afferent nerve action potentials once a predetermined mechanical threshold is reached. Our laboratory has developed a regenerative peripheral nerve interface (RPNI) to restore the sense of touch to neuroprostheses. However, precise replication of trains of afferent nerve action potentials is necessary to provide high fidelity, discriminable, graded somatosensory feedback. This work describes the transformation of a previous ex vivo experimental setup into an in vivo rat model for the purpose of capturing sensory recordings from the whole sural nerve. Back to Top
MATERIALS AND METHODS: A depilated neurocutaneous flap (peninsula-shaped skin paddle) in the sural nerve distribution was elevated off the dorsolateral aspect of the rat's foot leaving an intact skin bridge for its neurovascular supply. The flap was rotated into a skin-flap chamber containing phosphate-buffered saline, and secured to a silicone elastomer base. The purely sensory sural nerve was then exposed in the proximal thigh where in vivo multi-unit recordings were obtained, while a mechanical indenter fitted with a 3-mm diameter probe tip provided ramp- and-hold stimuli at depths of 1.0, 1.5, and 2.0 mm. Outcomes of interest included action potential firing rate and force application at the probe tip versus amount of displacement. Back to Top
RESULTS: Increasing amounts of displacement provided by the mechanical indenter resulted in increasing force detected by the load cell. As the amount of displacement increased, the action potential firing rate also increased over each stimulation period with 8.68, 42.00, and 61.10 spikes/s recorded at depths of 1.0, 1.5, and 2.0 mm, respectively. These findings indicate that the mechanical indenter is capable of producing differential action potential firing rates in the whole sural nerve by varying the amount of displacement. Back to Top
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CONCLUSIONS: In vivo afferent nerve action potentials were orthotopically recorded from the whole sural nerve using a novel skin-flap chamber, nerve conduction instrumentation, and a custom mechanical indenter. Varying the amount of displacement produced differential action potential firing rates demonstrating discriminable, graded somatosensory feedback. This experimental setup will serve as the foundation for future mechanical stimulation experiments to develop and validate an electrical stimulation algorithm for producing multi-unit recordings from the RPNI. Back to Top
ACKNOWLEDGEMENTS: The views expressed in this work are those of the authors and do not necessarily reflect official Army policy. This work was sponsored by the Plastic Surgery Foundation's Research Fellowship Grant and by the Defense Advanced Research Projects Agency under grant N66001-11-C-4190. ◀ Previous Article | Table of Contents | Next Article ▶ Copyright (c) 2000-2012 Ovid Technologies, Inc.
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