p Value Log2(FC) p Value Log2(FC) p Value Log2(FC)

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p Value Log2(FC) p Value Log2(FC) p Value Log2(FC)

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p Value. Log2(FC). CAT. 0.444097201 0.023091979 -1.257712046 0.000983546 0.386443774 0.041358057 CAT. -0.561615626 0.003558369 -0.386443774.

Changes compared to wild-type G6PD shRNA Grx1 shRNA

Trx1 shRNA Gene Symbol CAT GPX1 GSR PRDX1 PRDX2 PRDX4 APEX1 SRXN1 TXNRD1 GLRX GLRX2 TXN TXN2 G6PD IDH1 IDH2 AQP8 DUOX1 DUOX2 NOX1 CYBB NOX3 NOX4 NOX5 RAC1 RAC2 SOD1 SOD2

Log2(FC)

p Value

-0.319551129 0.025626635 -0.429704963

0.00137241

-0.585129081 0.0109731 -1.961231846 0.003534751

Log2(FC) p Value Log2(FC) p Value Log2(FC) p Value 0.444097201 0.023091979 -1.257712046 0.000983546 0.386443774 0.041358057 -1.431850433 0.00103646 -0.633456231 0.006342395 -1.960039775 -1.74936676 -0.42681853 0.041205272 -1.95269839 -1.184022268 -1.383929571 -2.006031036 -2.354386012 -0.730017665 0.002984928 -1.876590729 -1.525462786 -1.385533015 -1.462068242 0.002441014 -1.49723498

1.370188098 0.01391249 0.482473712 0.089380136 2.16922476 0.001517917

0.722553592 0.004247462 -0.613074599 0.001945752

pLKO

0.000356142 -0.643682798 0.00053749 5.84315E-05 0.000177952 -0.414676348 0.006335573 0.003632724 0.373452187 0.020380684 0.000466325 0.025687792 0.004228108 0.000223881 -0.674129168 0.005439961 0.003310809 0.015217843 0.002959574

-1.587453842 0.005694317 1.703991256 0.007574579

-0.539853732 0.00531766 -1.433111827 0.557637848 0.006498566 -1.33496666 -0.718765259 0.00411683 -1.804046949 -1.412471135

0.345824877 0.032086868 2.347567558 0.000666391

0.011035792 0.005201047 0.618503888 0.007873064 0.001216395 -0.736918132 0.008477113 0.002954379

Gene Symbol CAT GPX1 GSR PRDX1 PRDX2 PRDX4 APEX1 SRXN1 TXNRD1 GLRX GLRX2 TXN TXN2 G6PD IDH1 IDH2 AQP8 DUOX1 DUOX2 NOX1 CYBB NOX3 NOX4 NOX5 RAC1 RAC2 SOD1 SOD2

Trx1 shRNA

Changes compared to pLKO G6PD shRNA Grx1 shRNA

Log2(FC) p Value

Log2(FC)

-0.1626774 0.0277069

p Value

w.t.

Log2(FC) p Value Log2(FC) -0.561615626 0.003558369 -0.386443774

-0.233816783 -0.195821126 0.036637074 -0.614979426 -0.455481847 -0.47493426

0.036223713 0.643682798 0.000389564 0.002081694 0.414676348 0.002845608 -0.373452187

-0.6246443 0.0438786 -0.935614904 1.74131E-05 0.674129168 -1.8113858 0.0012555 -0.607021332 0.021386216 -1.597409566 0.003214057 -0.550036112 0.011471815 -0.850738525 0.002539775 -0.345824877 -0.643576304

0.01881708 -2.489041011 0.000499238 -2.347567558

-0.870930354 0.002953091 -0.618503888 0.736918132 -0.442469915 0.004187063