p53 controls cancer cell invasion by inducing the MDM2 ... - Nature

E R R AT U M p53 controls cancer cell invasion by inducing the MDM2-mediated degradation of Slug Shu-Ping Wang, Wen-Lung Wang, Yih-Leong Chang, Chen-Tu Wu, Yu-Chih Chao, Shih-Han Kao, Ang Yuan, Chung-Wu Lin, Shuenn-Chen Yang, Wing-Kai Chan, Ker-Chau Li, Tse-Ming Hong and Pan-Chyr Yang Received 29 October 2008; accepted 19 February 2009; published online 17 May 2009; corrected after print 19 May 2009 In the version of this article initially published, the Myc–Ub label in Figure 3c was incorret. Arrowheads were missing in fig. 5d and 5e. A plus (+) was misplaced from the curve in Fig. 7c. The correct versions of these figures are shown below. These errors have been corrected in the HTML and PDF versions of the article. Figure 5

Figure 3

d

* *

80 50 39 IB: Anti-HA 124

e

β-Actin

39

HA–Slug

MDM2: Vector

1–491 101–491 1–440

Input IgG Flag

Input IgG Flag

1–300

1–210

IB: SMP-14 *

*

*

Myc–Ub 5

Input IgG Flag

Flag–Slug

IP:

MDM2

4

Input IgG Flag

Anti-HA Anti-Flag

50

3

* IgGH *

* *

IB : Anti-HA Anti-p53 Anti-p53 Anti-HA Anti-HA Anti-HA

Input:

2

* *

HA–p53

HA–Slug

1

* *

*

*

** IgGL variants

IgGH

13

Input IgG Flag

IP :

+ + +

Input IgG SMP-14

+ + –

Input IgG SMP-14

+ – –

1–125 100–325 300–393

Input IgG SMP-14

+ + +

∆N96

Input IgG SMP-14

+ + –

∆C30

Input IgG SMP-14

+ – –

Ubiquitylated Slug

HA–Slug Myc–Ub MDM2 Mr(K) IB: Anti-Myc 182 124

+ MG132

Input IgG SMP-14

IP: anti-HA – MG132

HA–p53 : Vector 1–393

Input IgG Flag

H1299 (p53 )

Input IgG Flag

c

–/–

Anti-Flag

6

*

*

*IgGH MDM2 variants Flag–Slug

Figure 7 c

1.0 0.9

Probability of survival

0.8 0.7

p53mt–, M+, S–, E+ (n = 21)

0.6 0.5 0.4 0.3 0.2 0.1 0.0

914

p53mt+, M–, S+, E– (n = 14)

P = 0.048 0

10

20

30 40 50 Time (months)

60

70

80

nature cell biology volume 11 | number 7 | JULY 2009 © 2009 Macmillan Publishers Limited. All rights reserved.