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Alimentary Pharmacology and Therapeutics

Paediatric gastroenterology evaluation of overweight and obese children referred from primary care for suspected nonalcoholic fatty liver disease J. B. Schwimmer*,†,‡, K. P. Newton*,†, H. I. Awai*,†,‡, L. J. Choi*,†, M. A. Garcia*,†, L. L. Ellis§,¶,**, K. Vanderwall†† & J. Fontanesi‡‡,§§

*Division of Gastroenterology, Hepatology,

SUMMARY

and Nutrition, Department of Pediatrics, San Diego School of Medicine, University of California, San Diego, CA, USA. †

Department of Gastroenterology, Rady

Children’s Hospital San Diego, San Diego, CA, USA. ‡

Liver Imaging Group, Department of

Radiology, San Diego School of Medicine,

Background Screening overweight and obese children for non-alcoholic fatty liver disease (NAFLD) is recommended by paediatric and endocrinology societies. However, gastroenterology societies have called for more data before making a formal recommendation.

University of California, San Diego, CA, USA. §

Department of Pathology, Rady Children’s

Hospital San Diego, San Diego, CA, USA. ¶

Department of Pathology, San Diego School

of Medicine, University of California, La Jolla, CA, USA.

Aim To determine whether the detection of suspected NAFLD in overweight and obese children through screening in primary care and referral to paediatric gastroenterology resulted in a correct diagnosis of NAFLD.

**Department of Medical Sciences, Frank H. Netter MD School of Medicine at Quinnipiac University, Hamden, CT, USA. ††

Department of Anesthesiology, Rady

Children’s Hospital San Diego, San Diego, CA, USA. ‡‡

Division of General Internal Medicine,

Department of Medicine, Center for Management Science in Health, San Diego

Methods Information generated in the clinical evaluation of 347 children identified with suspected NAFLD through screening in primary care and referral to paediatric gastroenterology was captured prospectively. Diagnostic outcomes were reported. The diagnostic performance of two times the upper limit of normal (ULN) for alanine aminotransferase (ALT) was assessed.

School of Medicine, University of California, La Jolla, CA, USA. §§

Departments of Family and Preventive

Medicine and Pediatrics, San Diego School of Medicine, University of California, La Jolla, CA, USA.

Correspondence to: Dr J. B. Schwimmer, Division of Gastroenterology, Hepatology, and Nutrition,

Results Non-alcoholic fatty liver disease was diagnosed in 55% of children identified by screening and referral. Liver disease other than NAFLD was present in 18% of those referred. Autoimmune hepatitis was the most common alternative diagnosis. Children with NAFLD had significantly (P < 0.05) higher screening ALT (98  95) than children with liver disease other than NAFLD (86  74). Advanced fibrosis was present in 11% of children. For the diagnosis of NAFLD, screening ALT two times the clinical ULN had a sensitivity of 57% and a specificity of 71%.

Department of Pediatrics, University of California, 3020 Children’s Way, MC 5030

Conclusions

San Diego, CA 92123, USA.

Screening of overweight and obese children in primary care for NAFLD with referral to paediatric gastroenterology has the potential to identify clinically relevant liver pathology. Consensus is needed on how to value the risk and rewards of screening and referral, to identify children with liver disease in the most appropriate manner.

E-mail: [email protected]

Publication data Submitted 3 September 2013 First decision 6 September 2013 Resubmitted 13 September 2013 Accepted 13 September 2013 EV Pub Online 1 October 2013

Aliment Pharmacol Ther 2013; 38: 1267–1277

ª 2013 The Authors. Alimentary Pharmacology & Therapeutics published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. doi:10.1111/apt.12518

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J. B. Schwimmer et al. INTRODUCTION Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease in children.1 The diagnosis of NAFLD requires that 5% or more hepatocytes have macrovesicular steatosis, and that other liver diseases and/or clinical conditions, which may cause steatosis, are excluded.2 Approximately 25% of children with NAFLD have a progressive sub-phenotype known as non-alcoholic steatohepatitis (NASH).3 Some children with NASH will develop cirrhosis and end-stage liver disease.4–6 Thus, NAFLD is not a singular diagnosis, but a clinical–pathological diagnosis that encompasses a broad spectrum of liver disease ranging from isolated steatosis to steatohepatitis, fibrosis and cirrhosis.7 For the years 1993–2003, the prevalence of NAFLD in children aged 2–19 years was estimated to be 9.6%.1 Studies have consistently shown that obesity is one of the most important risk factors for paediatric NAFLD.1, 8–11 An overweight or obese child with elevated alanine aminotransferase (ALT) is typically considered to have suspected NAFLD.12 A recent report from the National Health and Nutrition Examination Survey demonstrated that the prevalence of suspected NAFLD in children age 12–19 in the United States more than doubled from 1988–1994 to 2007–2010.13 In 2005, a report from UC San Francisco and Stanford University noted that general paediatricians were ‘underscreening’ overweight children for NAFLD.14 Beginning in 2007, major medical societies published guideline statements regarding screening overweight and obese children for NAFLD.15–17 The positions of these societies are summarised in Table 1. Recommendations for screening have been made by paediatricians, endocrinologists and paediatric gastroenterologists. The paediatric guidelines state that overweight or obese children ≥10 years should be screened for NAFLD using serum ALT and aspartate aminotransferase (AST).18 There is, however, some controversy surrounding screening children for NAFLD. Using published literature through June 2011, the American Gastroenterology Association, American Association for the Study of Liver Diseases and the American College of Gastroenterology developed a Practice Guideline on the diagnosis and management of NAFLD, which was jointly published in Gastroenterology,19 Hepatology20 and American Journal of Gastroenterology21 in June 2012. The Practice Guideline states, ‘Due to a paucity of evidence, a formal recommendation cannot be made with regards to screening for NAFLD in overweight and obese children despite a recent expert

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Table 1 | Society guidelines regarding screening overweight and obese children for NAFLD Recommend screening children for NAFLD Society

Yes

American Academy of Family Physicians American Academy of Pediatrics American Association for the Study of Liver Disease American College of Gastroenterology American Gastroenterological Association Endocrine Society European Society for Pediatric Gastroenterology, Hepatology, and Nutrition National Association of Pediatric Nurse Practitioners North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition

X

No

Uncertain

Not stated

X X

X X

X X

X

X

committee recommendation for biannual screening for liver disease with liver enzyme measurements in this population’. In the absence of uniform guidance, physicians must make their own decision whether or not to screen overweight children for NAFLD. Many primary care providers are screening overweight and obese children for NAFLD and many of these children identified as having suspected NAFLD are referred to paediatric gastroenterology for evaluation. The diagnostic outcomes for such children with suspected NAFLD who were referred to paediatric gastroenterology have not been reported. Therefore, we sought to address critical gaps in the knowledge base with the following study aims: (i) To describe the population of children who were identified with suspected NAFLD through screening in primary care and referred to paediatric gastroenterology. (ii) To determine whether the detection of suspected NAFLD in overweight and obese children through screening in primary care and referral to paediatric gastroenterology resulted in a correct diagnosis of NAFLD.

Aliment Pharmacol Ther 2013; 38: 1267-1277 ª 2013 The Authors. Alimentary Pharmacology & Therapeutics published by John Wiley & Sons Ltd.

Evaluation of suspected NAFLD in children (iii) To determine the frequency of NASH amongst overweight and obese children who were identified with suspected NAFLD through screening in primary care and referred to paediatric gastroenterology. (iv) To determine the frequency of advanced fibrosis amongst overweight and obese children who were identified with suspected NAFLD through screening in primary care and referred to paediatric gastroenterology. (v) To determine the diagnostic performance of ALT two times the upper limit of normal (ULN) for the above outcomes amongst overweight and obese children who were identified with suspected NAFLD through screening in primary care and referred to paediatric gastroenterology.

METHODS Study population Screening for NAFLD and referral to paediatric gastroenterology were performed clinically in primary care prior to participation in the study. Eligibility for this study was designed to mirror the paediatric screening guidelines for NAFLD.18 Therefore, children had to be at least 10 years old and either overweight or obese. In the clinical notes from the primary care office, there had to be documentation of screening for NAFLD with ALT and referral to paediatric gastroenterology for evaluation of ‘elevated ALT’ or ‘suspected NAFLD’ or ‘NAFLD’. There was no study inclusion threshold set for ALT; rather, the determination that the screening ALT was abnormal was made by the primary care provider. The parent(s) of all subjects provided written informed consent. Written assent was obtained for all children. The protocol was approved by the institutional review boards of the University of California, San Diego and Rady Children’s Hospital San Diego. Standard of care clinical evaluation of suspected NAFLD The clinical evaluation of the children was at the discretion of the attending paediatric gastroenterologist and not dictated by research protocol. Information generated in the clinical evaluation of suspected NAFLD was captured prospectively. To provide clinical context for the study, details of this diagnostic process are provided. Paediatric gastroenterology clinic. All children underwent a comprehensive history and physical as part of the clinical consultation for suspected NAFLD. The history

included investigation of potential hepatotoxic medication intake as well as age-appropriate interviewing for relevant lifestyle factors including unprotected sexual activity, alcohol, tobacco and recreational drug use. Height and weight were measured. Physical examination also included assessment for signs of chronic liver disease.22–24 Initial laboratory studies performed in all children at least 1 month after screening labs included hepatic panel (ALT, AST, alkaline phosphatase, total protein, albumin, total bilirubin, direct bilirubin) and gamma glutamyl transferase (GGT) to assess the chronicity and nature of aminotransferase elevation; complete blood count to assess for anaemia and evidence of splenic sequestration related to portal hypertension; and coagulation studies to assess hepatic synthetic function. If this confirmatory testing showed continued evidence for liver disease, additional laboratory studies were performed as ordered by the paediatric gastroenterologist based on the clinical context. These labs included evaluation of both hepatic aetiologies [hepatitis A IgM, hepatitis B surface antigen, hepatitis B surface antibody, hepatitis C antibody, HIV ELISA, alpha-1 anti-trypsin phenotype, anti-nuclear antibody (ANA), anti-smooth muscle antibody (ASMA), anti-liver kidney microsomal antibody, quantitative IgG, ceruloplasmin, 24-h urinary copper measurement] and extra-hepatic aetiologies (tissue transglutaminase IgA, quantitative IgA, serum amino acids, urine organic acids, serum acylcarnitine, profile, creatine kinase, erythrocyte sedimentation rate, C-reactive protein, thyroid stimulating hormone and free thyroxine). In children who had no symptoms or signs of liver disease by history or physical examination, and all laboratory results were normal on confirmatory testing, further evaluation was not pursued, given low likelihood of liver disease. When there was evidence for chronic liver disease based on history, physical and/or laboratory testing, a percutaneous liver biopsy was offered for diagnosis.25

Liver biopsy. Children who underwent clinical liver biopsy did so according to the standard clinical protocol in use at our institution. Anaesthesia was provided by an attending paediatric anaesthesiologist. Children underwent a mask induction using a combination of gas ventilation with oxygen, nitrous oxide and sevoflurane. An intravenous line was placed and supplemental propofol and fentanyl was given according to the anaesthesiologist’s discretion. Hemodynamic monitoring was placed. Most patients were mask ventilated or had laryngeal mask airways placed. Ventilation was spontaneous.

Aliment Pharmacol Ther 2013; 38: 1267-1277 ª 2013 The Authors. Alimentary Pharmacology & Therapeutics published by John Wiley & Sons Ltd.

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J. B. Schwimmer et al. A time out was performed to confirm the correct patient and procedure. Children were positioned supine, with the right hand raised. Limited ultrasonography of the liver was performed to identify the ideal biopsy path. Liver biopsy was performed by an experienced paediatric gastroenterologist using a 15-gauge Jamshidi needle. A portion of tissue was placed in saline and brought afresh to pathology and a portion was placed in formalin for standard processing. After the procedure was completed, patients were taken to the post-anaesthesia care unit (PACU), monitored and observed for 4 h prior to discharge.

Pathology. Pathology procedures were performed according to standard clinical protocol within our institution. Formalin-fixed paraffin-embedded liver sections were stained with haematoxylin and eosin, periodic acid Schiff with and without diastase, Masson trichrome, reticulin and iron histochemical stains. Fresh frozen sections were stained with oil-red-O. Slides were evaluated systematically (adequacy, overall architecture, portal tracts, and parenchyma) by an experienced, board-certified, paediatric pathologist. Additional stains were performed and reviewed as needed based on the clinical context. Fibrosis was staged using standard methods relevant to the specific pathologic findings (e.g. Kleiner for NAFLD, METAVIR for viral hepatitis, etc.).26, 27 The pathological diagnosis was recorded. Diagnosis. The final diagnosis was made by the paediatric gastroenterologist incorporating all available information from clinical interview, medical record, physical examination, laboratory testing and review of histopathology. A diagnosis of NAFLD was based on exclusion of other causes of steatosis by clinical history, laboratory studies and histology in addition to histologic demonstration of ≥5% of hepatocytes containing macrovesicular fat. Following the prevailing standard, for those biopsies indicative of NAFLD, the diagnosis of steatohepatitis was based on the pathologists’ interpretation of the global histological features including steatosis, lobular and portal inflammation and ballooning degeneration of hepatocytes.26 The diagnoses of other liver diseases were made based on relevant society guidelines and standard gastroenterology, hepatology and pathology reference textbooks.24, 28, 29 For example, the diagnosis of autoimmune hepatitis was made following the recommendations of the AASLD Practice Guideline on the Diagnosis and Management of Autoimmune Hepatitis.30 1270

Data collection Information generated in the clinical evaluation of suspected NAFLD was captured prospectively. Age and sex were recorded. Because race and ethnicity influence the risk for NAFLD, each child’s race and ethnicity were self-identified by the parent(s). Height and weight were recorded. From the primary care provider’s office records, the screening ALT and AST values were recorded. In addition, from the paediatric gastroenterology clinical record, we prospectively recorded the values for laboratory assays performed for the evaluation of liver disease. Adverse events were recorded as problems in the operating room, patient complaints in the PACU, return to the hospital, calls to gastroenterology and by report at out-patient follow-up visit. Data analysis Calculated variables. Body mass index (BMI) was calculated as weight (kg) divided by height (m) squared. BMI percentiles were determined from the Centers for Disease Control and Prevention 2000 growth curves. BMI Z scores (s.d.s from the national reference mean for a given age and gender of children’s BMI values) also were determined. Definitions. Subjects were classified as overweight (BMI 85–94th percentiles) or obese (BMI ≥95th percentile). The most common value used at children’s hospitals in the United States for the ULN for ALT is 40 U/L.31 Therefore, two times the ULN was defined as 80 U/L. This value was used to assess the diagnostic performance of the paediatric guideline recommendation to use two times the ULN for ALT as the threshold for further evaluation. As a comparison, we also evaluated recently proposed biology-based thresholds for the ULN in children derived from the SAFETY study.31 These are gender-specific with ULN of 25 U/L in boys and 22 U/L in girls. Therefore, two times the biology-based ULN was defined as 50 U/L for boys and 44 U/L for girls. Advanced fibrosis was defined as bridging fibrosis or cirrhosis. Statistics. Data were expressed as mean  standard deviation (if not normally distributed, then geometric means were reported) or frequency and percentage. Continuous variables were analysed with Student’s t-test; the Mann–Whitney U test was used for nonparametric measures. The Pearson v2 test was used to test for differences in proportions. All hypothesis tests were two-tailed. Significance was defined a priori at a value of 0.05.

Aliment Pharmacol Ther 2013; 38: 1267-1277 ª 2013 The Authors. Alimentary Pharmacology & Therapeutics published by John Wiley & Sons Ltd.

Evaluation of suspected NAFLD in children Analyses were performed with Statistica 10 (StatSoft, Inc., Tulsa, OK, USA). We performed a post-hoc analysis of published indices for NAFLD and advanced fibrosis using readily available clinical data points.32 For the detection of NAFLD, we tested the Fatty Liver Index (FLI).33 The FLI was calculated as (e0.953*loge (triglycerides) + 0.139*BMI + 0.718*loge (GGT) + 0.053*waist circumference 15.745)/(1 + e0.953*loge (triglycerides) + 0.139* BMI + 0.718*loge (GGT) + 0.053*waist circumference 15.745) 9 100. The index produces a score from 1 to 100. Scores of 60 are considered positive for NAFLD. For the detection of advanced fibrosis, we tested the FIB-4 index.34, 35 This was calculated as (Age 9 AST)/(Platelets 9 (sqr (ALT)). Values