gastric H.pylori infection are available, but urea breath test. (UBT) with either 13C or 14C labelled urea is broadly used as it is simple, safe, non-traumatic, quick ...
IJNM, 17(1): 21-25, 2002 ORIGINAL ARTICLE
Influence of Delaying Gastric Emptying by Hyoscine N-Buttlbromide on 14C-Urea Breath Test Chander M Pathak, Deepak K Bhasin*, Brijesh C Sharma*, Kim Vaiphei@, Pallab Ray#, Baljinder Singh*, Krishan L Khanduja Departments of Biophysics, Gastroenterology*, Histopathology@, Medical Microbiology#, and Nuclear Medicine* Postgraduate Institute of Medical Education and Research, Chandigarh-160012, India. Optimal parameters for 14C-urea breath test for the diagnosis of Helicobacter pylori infection are still being worked out. The duration of the test, the timing of breath sampling and the accuracy of the method vary according to the test meal used for slowing the gastric emptying and to increase the area of contact with the substrate. In the present study, we investigated the impact of delaying gastric emptying by hyoscine Nbutylbromide (HBB), an atropine derivative, on the performance of 14C-UBT. A total of 17 patients of dyspepsia (13 having H.pylori infection) participated in the study. On three consecutive days, participants ingested 111 kBq(3 mCi) 14C-urea; first day with 200 ml of drinking water; second day 10 min after taking semi-liquid meal and on third day 5 min after i.v. administration of 20 mg HBB. 14C-UBT was performed on each occasion by collecting breath samples upto 60 min. Results were expressed as 14CO 2/mmol breath CO 2 x b.wt.(kg) as percent of administered dose. Rapid urease test and histological assessment of H.pylori presence in biopsy samples served as reference standard. The mean peak UBT values in H.pylori infected subjects with fasting, gastric loading and after HBB administration were 2.27 ± 0.34 at 5 min, 1.9 ± 0.35 at 45 min and 2.16 ± 0.15 at 15 min respectively, which were significantly different (P
