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Parameter Description Minimum value Base value - PLOS

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Comment

Maximum value kon,RJ. Association rate constant, receptor-Jak2. â. 0.06 nM-1min-1. â. KD,RJ = koff,RJ/kon,RJ. Dissociation constant, receptor-Jak2. 10 nM a.

Minimum value

Base value (if not varied)

Maximum value

—

0.06 nM-1min-1

—

10 nM a

100 nM

100 nM

—

0.06 nM-1min-1

—

1 nM b

100 nM

100 nM

—

0.06 nM-1min-1

—

0.1 nM b

100 nM

10 µM

Association rate constant, phosphoinositide-SH2-Bβ

—

0.06 nM-1min-1

—

KD,SP = koff,SP/kon,SP

Dissociation constant, phosphoinositide-SH2-Bβ

—

100 nM

—

JTot

Total Jak2 concentration

14 pM b

100 nM

100 nM

STot

Total SH2-Bβ concentration

0.01 nM b

100 nM

100 µM b

PTot

Total phosphoinositide concentration

0

1 µM

1 µM

kphos,slow

Jak2 transphosphorylation rate constant (Y2 of the kinase not phosphorylated)

—

6 min-1

—

kphos,fast

Jak2 transphosphorylation rate constant (Y2 of the kinase phosphorylated)

—

60 min-1

—

kdephos

Jak2 dephosphorylation rate constant

—

6 min-1

—

χm

Enhancement factor for interactions at the membrane

—

167

—

χr

Effective intra-complex concentration

—

100 µM

—

Parameter

Description

kon,RJ

Association rate constant, receptor-Jak2

KD,RJ = koff,RJ/kon,RJ

Dissociation constant, receptor-Jak2

kon,JS

Association rate constant, Jak2-SH2-Bβ

KD,JS = koff,JS/kon,JS

Dissociation constant, Jak2-SH2-Bβ

kon,SS

Association rate constant, SH2-Bβ dimerization

KD,SS = koff,SS/kon,SS

Dissociation constant, SH2-Bβ dimerization

kon,SP

Table S1. Model parameters. Growth hormone-receptor binding, receptor dimerization, and receptor trafficking parameters, not listed here, are from Biotechnology Progress, 20: 1337-1344 (2004). a Results not shown. b Extreme values considered for the In Vitro Model only.