PCR-Oligonucleotide Ligation Assay for Detection of Point Mutations ...

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Feb 25, 2002 - two target sequences compared with automated DNA se- quencing ... Mailing address: Dept. 47T ... on an automated sequencer (model 373A;.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Apr. 2003, p. 1456–1459 0066-4804/03/$08.00⫹0 DOI: 10.1128/AAC.47.7.1456–1459.2003 Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Vol. 47, No. 4

PCR-Oligonucleotide Ligation Assay for Detection of Point Mutations Associated with Quinolone Resistance in Streptococcus pneumoniae Mai-Ha Bui,* Gregory G. Stone,† Angela M. Nilius, Laurel Almer, and Robert K. Flamm Infectious Disease Research, Abbott Laboratories, Abbott Park, Illinois Received 25 February 2002/Returned for modification 13 October 2002/Accepted 30 December 2002

We have developed a PCR-oligonucleotide ligation assay to rapidly identify base substitutions in topoisomerase genes that are associated with quinolone resistance in clinical isolates of Streptococcus pneumoniae. Thirty-seven strains for which the ciprofloxacin MICs were >4 ␮g/ml and 16 strains for which the MICs were