BIOETANOL PRODUCTION FROM BAGASSE THROUGH. FUNGAL
TREATMENT COMBINATION OF TRICHODERMA. VIRIDE AND ASPERGILLUS
NIGER, ...
BIOETANOL PRODUCTION FROM BAGASSE THROUGH FUNGAL TREATMENT COMBINATION OF TRICHODERMA VIRIDE AND ASPERGILLUS NIGER, WITH ADDITION OF TRICHODERMA HARZIANUM, AND CONTINUED BY ZYMOMONAS MOBILIS IN FERMENTATION PROCESS Name of Student : MEY LIA (2308 100 078) ELIS SETIOWATI (2308 100 127) Department : Chemical Engineering, ITS Advisor : Ir. NUNIEK HENDRIANIE, M.T.
Abstract Along with the depletion of petroleum reserves, began an attempt to find alternatives to oil fuels, such as ethanol. Ethanol is a fuel that can be renewed, one potential feedstock for ethanol manufacture is bagasse. Bagasse is the residue of sugar cane that has been extracted in the mill and is one of the solid waste from sugar mills, bagasse is usually used as boiler fuel, bagasse can be used when one of the raw material producing ethanol. Bagasse contains substantial lignocellulosic materials, to reduce the lignin content of the bagasse is a necessary pretreatment process using the fungus Trichoderma harzianum, this fungus is a fungus which is good for delignification process. Meanwhile, to convert the material into bioethanol sesulosa necessary degradation of cellulose into glucose, the activity is done very well by Trichoderma viride and Aspergillus niger, while the fermentation process carried out by Zymomonas mobilis. Research is underway to take advantage of Bagasse as raw material for bioethanol through fungal treatment with Trichoderma viride combination and the addition of Aspergillus niger and Trichoderma harzianum, followed by Zymomonas mobilis fermentation process. This research method uses two main stages, namely, treatment and fungal fermentation. In this study using bagasse raw material and fungi Trichoderma viride, Trichoderma harzianum, Aspergillus niger, as well as the bacteria used for fermentation is Zymomonas mobilis. Bagasse initially washed,
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dried and milled and sieved to 100 mesh size is reached. Then do the manufacture of bagasse pulp with a ratio of bagasse and water, 250 g: 2500 ml were included in fungal treatment by aeration tank at 38C and pH 4-5.temperature of 38 Then added Trichoderma harzianum which has been cultured in liquid media for 42 hours, and left for 5 days. After the addition was done Trichoderma viride and Aspergillus niger which has been cultured in liquid media for 24 hours and left for 4 days. From the research that has been made known that Trichoderma viride dpat can degrade lignin up to 50,35% with the addition of 30% v / v, while for the addition of Trichoderma viride and Aspergillus niger is best to 3:1 v / v to produce glucose for 5,5 mg/l. The parameters analyzed include the initial conditions of bagasse (the content of cellulose, hemicellulose, and lignin). Conditions after the addition of Trichoderma harzianum (contains cellulose, hemicellulose, and lignin), and the condition after Trichoderma harzianum, Trichoderma viride and Aspergillus niger (the content of cellulose, hemicellulose, lignin, and glucose). Keywords: Aspergillus niger, Bagasse, Trichoderma harzianum, Trichoderma viride
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