Phase fraction calculation of microstructure using ImageJ
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Phase fraction calculation of microstructure using ImageJ
ImageJ. BY. HARIHARAN V S. HARIHARAN V S OPENMET IMAGEJ. 1 ... Adjust the scale such that only the phase of ... Select Analyzeâ Analyze Particles.
Phase fraction calculation of microstructure using ImageJ BY
HARIHARAN V S
HARIHARAN V S
OPENMET
IMAGEJ
1
STEP-1
1. Open ImageJ sotware 2. Select File Open
HARIHARAN V S
OPENMET
IMAGEJ
2
STEP-2
1. Select the required file 2. Click Open
HARIHARAN V S
OPENMET
IMAGEJ
3
STEP-3
The selected image appears
HARIHARAN V S
OPENMET
IMAGEJ
4
STEP-4
1.Select ImageDuplicate 2. Another copy of the image is created and file name prompt appears
HARIHARAN V S
OPENMET
IMAGEJ
5
STEP-5
1.Enter the name of duplicate file (which is of your wish) 2. Click OK 3. The duplicate image appears
HARIHARAN V S
OPENMET
IMAGEJ
6
STEP-6
Click on the rectangular box icon
HARIHARAN V S
OPENMET
IMAGEJ
7
STEP-7
The area other than the bottom unwanted area is selected
This area should not be counted for analysis HARIHARAN V S
OPENMET
IMAGEJ
8
STEP-8
Select Image Crop
HARIHARAN V S
OPENMET
IMAGEJ
9
STEP-9
Select ImageType8-bit
HARIHARAN V S
OPENMET
IMAGEJ
10
STEP-10
Select ImageAdjustThreshold
HARIHARAN V S
OPENMET
IMAGEJ
11
STEP-10
Adjust the scale to colour the phase of interest
HARIHARAN V S
OPENMET
IMAGEJ
12
STEP-11
Adjust the scale such that only the phase of interest is coloured red and then click APPLY
HARIHARAN V S
OPENMET
IMAGEJ
13
STEP-12
Select Analyze Analyze Particles
HARIHARAN V S
OPENMET
IMAGEJ
14
STEP-13 1.Ensure that “summarize” option is checked
2.Click OK
The phase fraction of shaded region is obtained
HARIHARAN V S
OPENMET
IMAGEJ
15
CAUTION The above procedure should be carried out for a large number of images taken in the same magnification and the average value should be reported. The obtained phase fraction will vary for different threshold values. Hence, it is necessary to choose the optimum threshold value which shades only the region/phase of interest. The images chosen for analyses should be clear and free of scratches. If the phases don’t have a sharp contrast difference, it will be difficult to get accurate results.
