Phenotypic, genotypic and antigenic characterization of emerging avian reoviruses isolated from clinical cases of arthritis in broilers in Saskatchewan, Canada Authors: Lisanework E Ayalew, Ashish Gupta, Jenny Fricke, Khawaja Ashfaque Ahmed, Shelly Popowich, Betty Lockerbie, Suresh K Tikoo, Davor Ojkic, Susantha Gomis* *
Corresponding author: 52 Campus Drive, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5B4. Phone #: +1-306-966-7299 E-mail address:
[email protected] Supplement Table 1. A list of ARV isolates and their Sigma C gene sequences deposited in GenBank Isolate
Symptom
GenBank Accession #
SK-R1 SK-R2 SK-R3 SK-R4 SK-R5 SK-R6 SK-R7 SK-R8 SK-R9 SK-R10 SK-R11 SK-R12 SK-R13 SK-R14 SK-R15 SK-R16 SK-R17 SK-R18 SK-R19 SK-R20 SK-R21 SK-R22 SK-R23 SK-R24 SK-R25 SK-R26 SK-R27 SK-R28 SK-R29 SK-R30 SK-R31 SK-R33 SK-R34 SK-R35 SK-R36 SK-R37 SK-R38
Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis
KX855899 KX855900 KX855901 KX855902 KX855903 KX855904 KX855905 KX855906 KX855907 KX855908 KX855909 KX855910 KX855911 KX855912 KX855913 KX855914 KX855915 KX855916 KX855917 KX855918 KX855919 KX855920 KX855921 KX855922 KX855923 KX855924 KX855925 KX855926 KY026178 KY026179 KY026180 KY026182 KY026183 KY026184 KY026185 KY026186 KY026187
1
Genotyping Cluster (Sigma C) VI VI VI VI VI VI VI VI VI V V V IV IV IV IV IV IV IV IV IV II II II II II II II VI II VI VI V II II VI II
Supplement Table 2. A list of ARVs and Sigma C gene sequences retrieved from the GenBank for this study Isolate
Country
Symptom
JR1 S1133 TU5 TU87 TU96 TU97.1 TU399 TU430 V.A.Vac 2408 601SI 1733 VA-1 ISR5220 ISR5226 ISR5225 04769a/14 04660/14 01384/14 04667/14 04666/14 ISR5233 GEL12 98M 601G R2-TW 918 916 GA41560 GA/12274 GEL13a98M 05273a/14 05273b/14 04455/13 01382/14 05287/14 GA/12355 GEL 13B98M ISR5233 GA41565 42563-4 28505a/11 28505b/11 22790/11 07634/14 28439/11 GEL05 97M NLA13 96T NLI12 96M GEL03 97T GEL01 96T
England USA Tunisia Tunisia Tunisia Tunisia Tunisia Tunisia USA USA Taiwan USA India Israel Israel Israel USA USA USA USA USA Israel Germany Taiwan Taiwan Taiwan Taiwan USA USA Germany USA USA USA USA USA USA Germany Israel USA USA USA USA USA USA USA Germany Netherlands Netherlands Germany Germany
Unclear Tenosynovitis MAS Unclear Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Vaccine MAS Tenosynovitis Tenosynovitis Vaccine Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis MAS Tenosynovitis Tenosynovitis MAS MAS RSS RSS MAS Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis RSS MAS Tenosynovitis RSS RSS Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis MAS Tenosynovitis MAS Tenosynovitis Tenosynovitis
2
GenBank Accession # EF122836.1 AF330703 HM751145 HM751144 HM751139 HM751140 HM751135 HM751137 EF122837 AF204945 AF204947 AF004857 EU681255.1 FJ793532 FJ793547 FJ793546 KP727766 KR856959 KR856956 KP727767 KP727768 FJ793549 AF354225.1 AF297217 AF297213 AF297215 AF297214 DQ872798 JX983599 AF354226.1
KR856981 KR856982 KP727778 KR856980 KR856986 JX983602 AF354227.1 FJ793549 DQ872799.1 DQ872801.1
KP727786 KR856990 KP727787 KR856992 KR856989 AF354223 AF354228 AF354230 AF354222 AF354221
Genotyping Cluster (Sigma C) I I I I I I I I I I I I I I I I I I I I I III I I I II II II II II II II II II II II III III III III III III III III III IV IV IV IV IV
02807/14 03795/14 04870/14 05573/12 05907/14 1017-1
03349/14 04314/14 05682/12 12323/13 GEL10 97M RAM-1 SOM-4
USA USA USA USA USA Taiwan USA USA USA USA Germany Australia Australia
Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis MAS Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis MAS Healthy Unclear
KP727807 KP727805 KP727806 KP727800 KR857002 AF297216
KR856994 KR856995 KP727791 KP727793 AF354219 L38502 L07069
IV IV IV IV IV V V V V V VI VI VI
Supplement Table 3. A list of ARV isolates and their Sigma B gene sequences deposited in GenBank Isolate
Symptom
GenBank Accession #
SigB_SK-R1 SigB_SK-R2 SigB_SK-R3 SigB_SK-R5 SigB_SK-R6 SigB_SK-R7 SigB_SK-R9 SigB_SK-R10 SigB_SK-R12 SigB_SK-R13 SigB_SK-R15 SigB_SK-R16 SigB_SK-R18 SigB_SK-R20 SigB_SK-R22 SigB_SK-R26 SigB_SK-R27
Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis Tenosynovitis
KY633770 KY633771 KY633772 KY633773 KY633774 KY633775 KY633776 KY633777 KY633778 KY633779 KY633780 KY633781 KY633782 KY633783 KY633784 KY633785 KY633786
3
Genotyping Cluster (Sigma C) VI VI VI VI VI VI VI V V IV IV IV IV IV II II II
4
5
6
7
8
