Accepted Manuscript “Phoenicibacter massiliensis” gen. nov., sp. nov. , a new bacterium isolated from the human gut of a pygmy woman Melhem Bilen, Frédéric Cadoret, Gregory Dubourg, Pierre-Edouard Fournier, Ziad Daoud, Didier Raoult PII:
S2052-2975(17)30033-1
DOI:
10.1016/j.nmni.2017.04.003
Reference:
NMNI 321
To appear in:
New Microbes and New Infections
Received Date: 13 February 2017 Revised Date:
5 April 2017
Accepted Date: 10 April 2017
Please cite this article as: Bilen M, Cadoret F, Dubourg G, Fournier P-E, Daoud Z, Raoult D, “Phoenicibacter massiliensis” gen. nov., sp. nov. , a new bacterium isolated from the human gut of a pygmy woman, New Microbes and New Infections (2017), doi: 10.1016/j.nmni.2017.04.003. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT “Phoenicibacter massiliensis” gen. nov., sp. nov. , a new bacterium isolated from the human gut of a pygmy woman
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Running title: Phoenicibacter massiliensis
Melhem Bilen1,2, Frédéric Cadoret1, Gregory Dubourg1, Pierre-Edouard Fournier1,
Aix-Marseille Université, URMITE, UM63, CNRS7278, IRD198, Inserm 1095,
M AN U
1
SC
Ziad Daoud2 and Didier Raoult1,3*
Institut Hospitalo-Universitaire Méditerranée-Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France •
2
Clinical Microbiology Laboratory, Saint George University Hospital, Faculty of
TE D
Health Sciences, University of Balamand, Beirut, Lebanon.
[email protected] 3
Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz
EP
•
AC C
University, Jeddah, Saudi Arabia
*
Corresponding author. E-mail address:
[email protected]
Keywords: Culturomics, “Phoenicibacter massiliensis”, Emerging bacteria, Gut microbiota, Human microbiota.
ACCEPTED MANUSCRIPT 1 2 3
Abstract:
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massiliensis” strain Marseille-P3241T (CSUR P3241); a new bacterium that was isolated from a
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stool sample of a healthy 47-year-old pygmy woman.
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This study supports the main characteristics of the new genus and new species “Phoenicibacter
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In 2015, stool samples were collected in Congo for analysis as part of project of the human
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microbiome description via culturomics [1]. Prior of the start up, an approval was taken from the
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ethic committee of the Institut Federatif de Recherche IFR48 (Marseille, France) under the
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number 09-022. Thus, 1 mL of phosphate buffer saline was used to dilute stool samples ahead of
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inoculation in a blood culture media supplemented with 5mL of filtered rumen and 5mL of sheep
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blood. Culture bottle was incubated at 37°C under anaerobic conditions. After 10 days of growth,
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strain Marseille-P3241 was isolated on 5% blood–enriched Columbia agar (bioMérieux, Marcy
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l'Etoile, France). Colonies were smooth with a diameter ranging between of 0.1-0.5 mm. Strain
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Marseille-P3241 cells are cocco-bacillus, Gram positive, catalase and oxidase negative with a
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diameter of an average of 2.9 µm. This strain could not be identified using our systematic matrix-
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assisted laser desorption-ionization time-of-flight (MALDI-TOF) screening on a Microflex
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spectrometer (Bruker Daltonics, Brenen, Germany) [2]. Therefore, sequencing of the 16S rRNA
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gene was performed by the mean of the fD1-rP2 primers as previously described (Eurogentec,
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Seraing, Belgium), using a 3130-XL sequencer (Applied Biosciences, Saint Aubin, France) [3].
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Strain Marseille-P3241 exhibited an 87.7% sequence identity with Gordonibacter pamelaeae the
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phylogenetically closest species, published with standing nomenclature (Fig. 1). Having a 16S
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rRNA gene sequence similarity
