Physiological Induction of Transient Receptor Potential Canonical

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trial smooth muscle; Mr, relative molecular mass; NP, nonpregnant; N.S., not significant ... the induction of calcium oscillations and non-SOCE/SOCE. Subjects ...
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The Journal of Clinical Endocrinology & Metabolism 89(3):1291–1300 Copyright © 2004 by The Endocrine Society doi: 10.1210/jc.2003-031428

Physiological Induction of Transient Receptor Potential Canonical Proteins, Calcium Entry Channels, in Human Myometrium: Influence of Pregnancy, Labor, and Interleukin-1␤ A. DALRYMPLE, D. M. SLATER, L. POSTON,

AND

R. M. TRIBE

Parturition Research Group (A.D., L.P., R.M.T.), Maternal and Fetal Research Unit, Department of Women’s Health, Guy’s, King’s and St. Thomas’ School of Medicine, St. Thomas’ Hospital Campus, London, SE1 7EH, United Kingdom; and Biochemical Research Institute (D.M.S.), The University of Warwick, Coventry, CV4 7AL, United Kingdom This study investigated gestational regulation of transient receptor potential canonical (TrpC) proteins, putative calcium entry channels in human myometrium, and the potential modulation of TrpC expression by IL–1␤, a cytokine implicated in labor. Total RNA and proteins were isolated from myometrial biopsies obtained from NP women, pregnant women at term not in labor (TNL), or term active labor (TAL) and from primary cultured human myometrial smooth muscle cells incubated with IL–1␤ or IL–1␤ with or without nimesulide. Semiquantitative RT-PCR demonstrated significant upregulation of TrpC1 in TAL and TNL (P < 0.01) and TrpC6 (P