Plasma soluble tumor necrosis factor alpha receptors and ... - CiteSeerX

12 downloads 0 Views 195KB Size Report
Plasma soluble tumor necrosis factor alpha receptors and leptin ... sTNFa receptor 1 (sTNFR1) was higher in morbid obese diabetic individuals compared with ...
European Journal of Endocrinology (2002) 146 325–331

ISSN 0804-4643

CLINICAL STUDY

Plasma soluble tumor necrosis factor alpha receptors and leptin levels in normal-weight and obese women: effect of adiposity and diabetes Mo`nica Bullo´, Pilar Garcı´a-Lorda and Jordi Salas-Salvado´ Unitat de Nutricio´ Humana, Hospital Universitari de Sant Joan de Reus, Facultat de Medicina i Cie`ncies de la Salut de Reus, Universitat Rovira i Virgili, C/Sant Llorenc¸, 21, 43201 Reus, Spain (Correspondence should be addressed to J Salas-Salvado´; Email: [email protected])

Abstract Objective: To explore the determinants of the tumor necrosis factor alpha (TNFa) system and their relationship with plasma leptin levels. Methods: We studied a cohort of 157 diabetic and non-diabetic females with a wide range of adiposity distributed into five groups: control – body mass index (BMI) between 19 and 27 kg/m2 ðn ¼ 24Þ; obese – BMI between 27 and 40 kg/m2 ðn ¼ 63Þ; obese type 2 diabetes mellitus – BMI between 27 and 40 kg/m2 with diabetes mellitus ðn ¼ 19Þ; morbid obese – BMI .40 kg/m2 ðn ¼ 29Þ; and morbid obese type 2 diabetes mellitus – BMI .40 kg/m2 with diabetes ðn ¼ 22Þ: Fasting glucose levels, plasma total triglycerides and cholesterol, high-, low- and very low-density lipoprotein cholesterol were assayed by enzymatic and colorimetric methods. Plasma TNFa levels were measured by ELISA assay and insulin and leptin levels by radioimmunoenzymatic assays. Both soluble TNFa (sTNFa) receptors were measured by immunoenzymometric assays. Results: All groups of patients showed significant increases in both sTNFa receptors relative to control. sTNFa receptor 1 (sTNFR1) was higher in morbid obese diabetic individuals compared with their nondiabetic counterparts ðP ¼ 0:003Þ; while sTNFR2 was significantly different between obese and morbid obese subjects ðP ¼ 0:036Þ: Bivariate correlation analysis showed a significant relationship between both plasma sTNFa receptors and BMI, percentage of body fat, fasting glucose, insulin and leptin. In multivariate analysis, both sTNF receptor plasma levels were predicted by percentage of body fat and the presence of diabetes (R 2 ¼ 0:20 for sTNFR1 and sTNFR2). When plasma leptin levels were added into the model, this protein and the presence of diabetes explained 27% of the variance of the plasma sTNFR1 levels. Conclusion: The presence of diabetes, adiposity or leptin levels are independent determinants of both sTNFa receptors. The independent association between plasma TNFa receptors and leptin levels in obese patients is consistent with the hypothesis that these proteins could be involved in the same pathway that regulates body adiposity. European Journal of Endocrinology 146 325–331

Introduction Tumor necrosis factor alpha (TNFa), a cytokine mainly produced by immunological cells, has been implicated in the regulation of energy balance in different pathological conditions such as cancer (1), AIDS (2, 3) and other catabolic situations (4). This cytokine is also produced by adipose (5) and muscle tissues (6), and has recently been involved in the pathophysiology of obesity (7 –9). TNFa expression is increased in most of the rodent models of obesity examined to date (5). This increase in the adipose and muscle tissue TNFa expression has also been reported in obese people (6, 10) together with significant relationships between TNFa expression

q 2002 Society of the European Journal of Endocrinology

(or plasma protein product concentrations) and adiposity (11, 12). However, a considerable number of investigators have been unable to observe higher TNFa levels in obese compared with lean individuals (13). Because the levels of soluble TNFa (sTNFa) receptors have been considered a reflection of the activity of the TNFa system (14), different studies have attempted to evaluate the effect of adiposity on these proteins and their findings have been controversial. Some smallsized studies have shown an increase in both soluble receptors (12, 13) or only in sTNFa receptor 2 (sTNFR2) (15 –17) in obese subjects compared with controls. Furthermore, in a recent report developed in a large sample, no significant differences in both

Online version via http://www.eje.org

326

M Bullo´ and others

EUROPEAN JOURNAL OF ENDOCRINOLOGY (2002) 146

plasma sTNFa receptors were observed between lean and overweight men (18). The expression and levels of these molecules have been related to glucose homeostasis (18), degree of hyperinsulinemia (15) or leptinemia (12), suggesting that the TNFa system plays a central role in the insulin resistance associated with obesity, but the effect in obese people of insulin sensitivity or the presence of diabetes on both plasma sTNFa receptors has not been well studied to date. We report here the largest study to date performed on a group of diabetic and non-diabetic females with a wide range of adiposity, with the aim of exploring the factors that determine the TNFa soluble receptor plasma levels, including age, adiposity, degree of hyperinsulinemia or leptinemia and the presence of diabetes.

Materials and methods Subjects We studied 157 females aged between 19 and 65 years with a wide range of adiposity and who were consecutively recruited from among those attending the outpatient clinics of the Hospital Universitari de Sant Joan de Reus, Spain. Four patient-group assignments, with respect to body mass index (BMI) and presence/ absence of diabetes, were compared with a control group of healthy subjects with a BMI between 19 and 27 kg/m2. The subjects were defined as obese when the BMI was between 27 and 40 kg/m2 and as morbid obese when the BMI was .40 kg/m2 according to the Spanish Consensus for obesity diagnosis and treatment (19). The presence of diabetes was confirmed when a previous diagnosis of non insulin-dependent diabetes mellitus (NIDDM) had been performed or when fasting plasma glucose of .7 mmol/l was observed on two consecutive occasions. Glycated hemoglobin $7.0 was considered as diabetes under poor metabolic control (20). Eighty-four patients were premenopausal and 73 were post-menopausal females. The characteristics of all the study subjects are summarized in Tables 1 and 2. Exclusion criteria

were the presence of infectious, inflammatory, neoplastic or systemic diseases, hypothyroidism or endocrine diseases other than diabetes, or the active use of anti-obesity drugs. The study protocol was approved by the hospital ethics committee and all subjects gave informed written consent to their participation in the study.

Data collection and laboratory procedures Height was measured without shoes to the nearest 0.5 cm. Body weight was measured to the nearest 0.1 kg (with the subject lightly clothed) and BMI was calculated as weight/height2. Waist circumference was measured midway between the lower rib margin and the iliac crest. Hip circumference was determined as the widest circumference measured over the greater throcanter. The waist-to-hip ratio (WHR) was then calculated. Fat free mass (FFM) was assessed as previously described by tetrapolar bioelectrical impedance at 50 kHz (Human-In ScanR, Dietosystem, Madrid, Spain) using the gender and fat-specific equations validated by Segal et al. (21). Fat mass (FM) was then calculated as the difference between total body weight and FFM. Fasting blood samples were collected in the morning between 0800 h and 1000 h. Plasma and serum were separated immediately by centrifugation and aliquots were frozen at 280 8C for subsequent batched analysis. Plasma total triglycerides and cholesterol, high-, lowand very low-density lipoprotein cholesterol (HDL, LDL and VLDL cholesterol) and glucose levels were assayed by the hospital’s routine chemistry laboratory. A commercial RIA kit was used to determine fasting plasma insulin (Amersham, Little Chalfont, Bucks, UK). The lowest limit of detection was 48 pg/ml. The intra- and inter-assay coefficients of variation for insulin were 5.05% and 13.4% respectively. Homeostasis model assessment of insulin resistance (HOMA IR) was then calculated as previously described (12): HOMA IR ¼ ½fasting insulin ðU=mlÞ £ fasting glucose ðmmol=Lފ=22:5 Immunoenzymometric assays were used to determine the levels of sTNFR1 and sTNFR2 concentrations in

Table 1 Anthropometric and biometric characteristics of the study subjects. Values are expressed as means (S.E.M. ).

Age (years) Height (cm) BMI (kg/m2) Body fat (%) Waist (cm) WHR

Control ðn ¼ 24Þ

Obese ðn ¼ 63Þ

Obese type 2 diabetes mellitus ðn ¼ 19Þ

Morbid obese ðn ¼ 29Þ

Morbid obese type 2 diabetes mellitus ðn ¼ 22Þ

38.4 (2.3) 161.8 (1.3) 23.0 (0.5) 26.2 (0.6) 78.2 (7.8) 0.81 (0.01)

46.3 (1.3)* 157.8 (0.8)* 34.2 (0.5)** 44.6 (0.3)** 103.4 (11.06)** 0.90 (0.01)**

51.6 (2.6)** 159.0 (1.2) 34.6 (0.7)** 44.9 (0.5)** 109.8 (9.83)**† 0.94 (0.01)**†

41.5 (2.2)‡ 158.0 (1.2)* 45.6 (0.7)**‡‡ 50.1 (0.3)**‡‡ 119.1 (11.10)**‡‡ 0.85 (0.01)‡

46.3 (1.8)* 158.3 (1.2) 47.3 (1.3)**‡‡ 50.1 (0.4)**‡‡ 122.4 (8.89)**‡‡ 0.91 (0.01)**†

Comparisons were performed using one-way ANOVA with multiple comparisons. *P , 0:05; **P , 0:001 vs control; †P , 0:05; ††P , 0:001 vs corresponding non-diabetic group; ‡P , 0:05; ‡‡P , 0:001 vs obese or obese type 2-diabetes mellitus.

www.eje.org

TNFa – leptin system, adiposity and diabetes

EUROPEAN JOURNAL OF ENDOCRINOLOGY (2002) 146

327

Table 2 Biochemical characteristics of the study subjects.

Fasting glucose (mmol/l) Plasma cholesterol (mmol/l) HDL cholesterol (mmol/l) VLDL cholesterol (mmol/l) LDL cholesterol (mmol/l) Triglycerides (mmol/l) Fasting insulin (mU/ml) HOMA IR Plasma leptin (ng/ml) TNFa (pg/ml)

Control ðn ¼ 24Þ

Obese ðn ¼ 63Þ

Obese type 2 diabetes mellitus ðn ¼ 19Þ

Morbid obese ðn ¼ 29Þ

Morbid obese type 2 diabetes mellitus ðn ¼ 22Þ

5.1 (0.08) 5.2 (0.21) 1.9 (0.08) 0.4 (0.04) 3.0 (0.18) 0.9 (0.08) 7.58 (1.00) 1.9 (0.24) 9.1 (1.00) 5.7 (2.02)

5.6 (0.06)** 5.9 (0.13)* 1.5 (0.05)** 0.6 (0.03)** 3.7 (1.13)* 1.4 (0.08)** 14.45 (1.00)** 4.7 (0.5)** 25.7 (1.00)** 11.6 (2.40)

8.5 (0.53)**†† 5.7 (0.24) 1.4 (0.10)** 0.8 (0.11)* 3.4 (0.25) 1.9 (0.27)* 19.05 (1.02) 13.7 (6.6) 22.4 (1.01)** 15.3 (4.00)

5.5 (0.11)* 5.5 (0.20) 1.5 (0.07)* 0.6 (0.05)* 3.3 (0.14) 1.4 (0.12)* 28.8 (1.01)*‡ 12.1 (2.3)*‡ 51.3 (1.00)**‡‡ 8.6 (2.30)

8.5 (0.65)**†† 5.5 (0.20) 1.4 (0.08)** 0.8 (0.09)** 3.1 (0.15)‡ 1.9 (0.20)**† 28.18 (1.01)** 7.31 (1.6)** 43.6 (1.01)**‡ 12.0 (3.20)

Values are expressed as means (S.E.M. ). Comparisons were performed using one-way ANOVA with multiple comparisons. *P , 0:05; **P , 0:001 vs control; †P , 0:05; ††P , 0:001 vs correspondent non diabetic group; ‡P , 0:05; ‡‡P , 0:001 vs obese or obese type 2 diabetes mellitus.

plasma (BioSource, Fleunes, Belgium). The minimum detectable concentration was estimated to be 50 pg/ ml and 0.1 ng/ml for each of the two receptors respectively. The intra- and inter-assay coefficients of variation were ,6.5% and ,8.9% respectively for sTNFR1 and ,3.3% and ,6.9% respectively for sTNFR2. TNF levels were measured by ELISA (Pharmingen, San Diego, CA, USA). The intra- and inter-assay coefficients of variation were ,5.8% and ,13.8% respectively, and the limit of sensitivity was 4 pg/ml. Plasma leptin levels were measured by a radioimmunoenzymatic assay using commercial kits (Linco Research, St Louis, MO, USA). Within- and between-assay variations were 4.98% and 4.5% respectively and the limit of sensitivity was 0.5 ng/ml.

Statistical methods Descriptive results of continuous variables are expressed as means^s:e:m: The variables with nongaussian distributions, such as leptin and insulin levels, were logarithmically transformed to approach normal distribution. The means are expressed as geometric means. One-way ANOVA with multiple comparisons was used to explore differences between groups. Relationships between two quantitative variables were assessed by Pearson’s correlation coefficient. A stepwise multiple regression analysis was performed to identify the factors affecting plasma levels of sTNFa receptors. Age, percentage of fat mass, WHR, fasting plasma glucose and insulin concentrations, and presence/absence of diabetes mellitus were entered as independent variables. The model R 2 indicates the percentage variance in the dependent variable that is explained by the independent variables included in the model. Plasma leptin levels were introduced in a second model exploring the determinants of both sTNFa receptors. All statistical calculations were performed using the SPSS/PC software. Values were considered statistically significant with P , 0:05:

Results Table 1 contains the anthropometric characteristics of the study groups. BMI, percentage of body fat and waist circumference increased in relation to the degree of obesity. No significant differences in anthropometric variables were observed in relation to the presence or absence of diabetes except a higher WHR in diabetic patients relative to non-diabetic patients. Diabetic patients were treated either by diet alone ðn ¼ 28Þ or by oral anti-diabetic agents ðn ¼ 11Þ: Only one diabetic patient required insulin at the time of the study. Glycemic control (Hba1c ,7.0%) was good in 14 patients (12 patients were put on a diet and two were treated with metformin) and poor in 25 patients (16 were put on a diet, five were treated with sulfonylurea and four were treated with metformin). None of the patients were taking thiazolidenedione during the study. Fasting glucose levels were significantly increased in patients with diabetes mellitus compared with controls and their obese counterparts. Plasma total cholesterol, HDL cholesterol, VLDL cholesterol and triglycerides were increased in all groups of patients compared with controls. Increases in plasma leptin and insulin levels were observed in morbid obese patients in relation to obese and control subjects (Table 2). No significant differences were observed between groups in relation to plasma TNFa levels. All groups of patients showed a significant increase in both plasma sTNFa receptors compared with control ðP , 0:001Þ; even after the data had been adjusted for age (data not shown). As shown in Fig. 1, sTNFR1 was higher in morbid obese diabetic individuals compared with obese ðP , 0:001Þ or morbid obese non-diabetic patients ðP ¼ 0:003Þ: In relation to the sTNFR2, a significant mean difference was observed between the obese and the morbid obese patients ðP ¼ 0:036Þ; obese type 2 diabetic group ðP ¼ 0:006Þ; and morbid obese type 2 diabetic group ðP , 0:001Þ: Morbid obese diabetic patients showed higher sTNFR2 levels than www.eje.org

328

M Bullo´ and others

their non-diabetic counterparts, although the differences were not statistically significant ðP ¼ 0:065Þ: After adjusting for adiposity, both sTNFa receptors were higher in obese diabetic patients ðn ¼ 41; 2.11^0.09 ng/ml for sTNFR1 and 5.66^0.26 ng/ml for sTNFR2) than non-diabetic obese patients (1.85^0.06 ng/ml for sTNFR1 and 4.75^0.17 ng/ml for sTNFR2; P ¼ 0:016 and P ¼ 0:006 respectively). No significant differences were observed in plasma leptin levels or either sTNFa receptor levels between patients with good or poor glycemic control or between patients who had been put on a diet and those who were treated with oral hypoglucemiants, even after adjusting for differences in adiposity (data not shown). Neither did the type of antihypertensive therapy lead to any significant differences in sTNFa receptors, plasma insulin levels or HOMA IR. No significant differences were observed in leptin or sTNFa receptor levels between pre- and post-menopausal patients, after data had been adjusted for the percentage of body fat and age (data not shown). Bivariate correlation analysis showed a positive relationship between both plasma sTNFa receptors and BMI, percentage of total body fat, fasting glucose, glycated hemoglobin, HOMA IR, leptin (Fig. 2) and insulin; and a negative correlation with HDL cholesterol levels (Table 3). We also observed a significant correlation between levels of sTNFR2 and WHR (Table 3). By contrast, no significant relationship was observed between plasma TNFa levels and any of the quantitative variables measured.

EUROPEAN JOURNAL OF ENDOCRINOLOGY (2002) 146

Finally, we performed a multiple linear regression in which plasma sTNFR1 and sTNFR2 were introduced as dependent variables, while age, percentage of body fat, WHR, fasting insulin and the presence of diabetes were the independent variables. Both plasma sTNFa receptor levels were significantly predicted by the percentage of body fat and the presence or absence of diabetes ðR 2 ¼ 0:20 and R 2 ¼ 0:19; P , 0:001 for sTNFR1 and sTNFR2). When plasma leptin levels were added to the previous model, the significant predictors of sTNFR1 and sTNFR2 were leptin levels and the presence of diabetes ðR 2 ¼ 0:27; R 2 ¼ 0:23; P , 0:001 respectively). Similar results were obtained when only obese patients were analyzed (data not shown).

Discussion Increasingly in the literature, there has been the suggestion that TNFa could be involved in the pathogenesis of insulin resistance associated with obesity. However, there has not been any consensus and several investigators have failed to demonstrate higher circulating levels of this cytokine, in this metabolic situation, compared with controls (13, 22). Similarly, in our study we did not observe any significant differences in levels of this cytokine between the obese patients and control subjects. The paracrine mechanism of TNFa secretion, the highly labile nature of this protein and the methodological difficulties of its quantification could be among the factors that may explain this.

Figure 1 Plasma levels of both sTNFa receptors in each study group. Values are expressed as means (error bars represent S.E. ). *P , 0:05; **P , 0:001: Mean values are significantly increased in all groups compared with controls ðP , 0:001Þ:

www.eje.org

TNFa – leptin system, adiposity and diabetes

EUROPEAN JOURNAL OF ENDOCRINOLOGY (2002) 146

329

Figure 2 Relationship between both sTNFa receptors and plasma leptin levels in the overall study group.

The assessment of the TNFa system activity based on the plasma TNFa receptor levels appears to be more reliable and of greater interest than plasma TNFa concentration itself. These proteins are easily detectable in plasma and appear to act as a buffer system prolonging the biological effects of TNFa (14, 23) and seem to reflect more accurately the degree of TNFa system activation than the concentrations of circulating TNFa (23). Thus, we have observed significantly higher levels of both sTNFa receptors in obese, and especially in morbid

obese, subjects compared with normal-weight subjects. Even when there is a general consensus in the increased levels of sTNFR2 in obesity (12, 13, 15 – 17), only a few studies have reported an increase in the sTNFR1 in obese patients compared with lean healthy controls (12, 13). A possible explanation for these discrepancies could rely on the limited sample size of some of these previous studies (15, 17). It is interesting to note that in the largest sample of individuals reported in the literature to date ðn ¼ 178Þ no significant differences have been observed in either

Table 3 Bivariate regression analysis between plasma leptin levels, TNFa and sTNFa receptors and selected variables in the whole population.

Age (years) BMI (kg/cm2) WHR Total body fat (%) Fasting glucose (mmol/l) HGa1c (%) Plasma cholesterol (mmol/l) HDL cholesterol (mmol/l) VLDL cholesterol (mmol/l) LDL cholesterol (mmol/l) Triglycerides (mmol/l) Leptin (ng/ml) Insulin (mU/ml) HOMA IR

Leptin (ng/ml)

sTNFR1 (ng/ml)

sTNFR2 (ng/ml)

ns 0.738** ns 0.724** ns ns ns 20.310** 0.187* ns 0.178* – 0.544** 0.214**

ns 0.416** ns 0.406** 0.204* 0.240* ns 20.290** ns ns ns 0.492** 0.276** 0.209*

ns 0.356** 0.187* 0.356** 0.301** 0.437** ns 20.328** ns ns 0.263** 0.372** 0.296** 0.392**

*P , 0:05; **P , 0:001:

www.eje.org

330

M Bullo´ and others

soluble receptor between lean and overweight subjects. In contrast to our results, the aforementioned study was performed exclusively in men, suggesting that gender plays a pivotal role in determining the levels of these soluble receptors (18). In this study, the percentage of body fat was the best predictor of the levels of both soluble receptors in the overall study population as well as in the obese patients considered apart. This is in agreement with previous studies showing that BMI was the best determinant of sTNFa receptor levels (12, 13, 16, 17). In contrast, this is the first study to demonstrate that the levels of these receptors are higher in diabetic than in nondiabetic females, independent of the degree of adiposity. In fact, the presence/absence of diabetes was a significant predictor of both soluble forms of TNFa receptors. Some authors have demonstrated a relationship between fasting insulin or HOMA IR and sTNFR1 (12) or sTNFR2 (12, 13) in obese patients. Ferna´ndez-Real et al. (17) further demonstrated a negative correlation between insulin sensitivity and sTNFR2. In our study, a positive and significant relationship was observed between both soluble receptors and insulin plasma levels or HOMA IR in the overall study population and this association was maintained for the sTNFR2 alone when the patient groups were assessed separately. The increase in the levels of these receptors, and their relationship to the degree of insulin resistance, could be viewed as resulting from an increase in the production and activity of the TNFa system, secondary to an excess of adiposity, in order to breakdown the expansion of fat depots. Indeed, it has been observed that TNFa can decrease adipose tissue lipoprotein lipase activity (24, 25) and can also induce insulin resistance through its ability to produce serine phosphorylation of insulin receptor substrate 1, thus decreasing the tyrosine kinase activity of the insulin receptor (26). Both mechanisms are responsible for a fall in adipocyte and/or muscle tissue fuel bioavailability and of an increase in lipolysis (27). Another interesting aspect of our study is the observation that there is a significant relationship between sTNFR1 or sTNFR2 and plasma leptin levels either when the overall study population is considered or only in the obese patient group. Other authors have shown bivariate correlations between leptin and sTNFR2 (18, 28) or both sTNFa receptors (12) in obese patients or healthy and diabetic subjects. Further, when we included the values of leptin concentrations as an independent variable in the multiple regression analysis model, both leptin levels and the presence of diabetes were the only independent predictors of sTNFR1 and sTNFR2. These observed relationships between the proteins suggest a certain degree of overlapping between leptin and TNFa. The rationale is that, first, both proteins are produced by adipose tissue in proportion to the www.eje.org

EUROPEAN JOURNAL OF ENDOCRINOLOGY (2002) 146

amount of fat stores (10, 29) and are capable of regulating energy intake and expenditure (30). Secondly, a decrease in adipocyte expression and plasma concentrations of both proteins have been observed after weight loss (29) and these levels can increase during re-feeding/re-nutrition (10) and which indicates that similar mechanisms are involved in the regulation of these proteins. Finally, leptin synthesis can be modulated by the administration of TNFa in vitro (31, 32) and in vivo (33), although contradictory data have been published in the literature. An increase in the adipocyte production of leptin after TNFa incubation was observed by Kirchgessner et al. (31). However, Zumbach et al. (33) found only a transient increase in serum leptin production with administration of TNFa and, recently, an inhibition in leptin synthesis was reported after long-term TNFa adipocyte incubation (32, 34). Even though this study shows the largest sample of obese females to date and includes a group of severely obese patients, with or without diabetes, the study does have some limitations. First, the cross-sectional design of the study has not allowed us to infer causal relationships. In addition, the bioelectrical impedance analysis (BIA) prediction of body fat mass presents some methodological flaws especially in morbid obese patients. However, when the BMI is used instead of percentage of body fat, the same magnitude of relationship has been observed. Finally, due to the difficulties in recruiting lean type 2 diabetic patients, we are not able to state whether these relationships would still be maintained in this subpopulation. To conclude, TNFa receptors are increased in obese and diabetic patients as a reflection of the degree of adiposity and associated insulin resistance. The presence of diabetes, adiposity or leptin levels are independent determinants of both sTNFa receptors. The independent association between plasma TNFa receptors and leptin levels in obese patients is consistent with the hypothesis that these proteins could be involved in the same pathway directed to regulate body adiposity. Further studies are necessary to elucidate the implications of the TNF– leptin system in the etiology and metabolic derangements associated with obesity.

Acknowledgements We would like to thank Dr A Bonada and I Megı´as for their technical support and Carles Munne´ for manuscript preparation. This study was supported by the Fondo de Investigacio´n Sanitaria (FIS 99/0284) of the Ministerio de Sanidad y Consumo. Mo`nica Bullo´ was in receipt of a fellowship from the Comissionat per a Universitats i Recerca de la Generalitat de Catalunya (CURGC 1999FI 00946PG).

EUROPEAN JOURNAL OF ENDOCRINOLOGY (2002) 146

References 1 Keller U. Pathophysiology of cancer. Supportive Care in Cancer 1993 1 290 –294. 2 Salehian B, Niyongabo, Salmon D, Vilde JL, Melchior JC, Rigaud D et al. Tumor necrosis factor and resting energy expenditure during the acquired immunodeficiency syndrome. American Journal of Clinical Nutrition 1993 58 715–716. 3 Salazar JF, Martinez O, Aziz N, Kolberg JA, Yeghiazarian T, Lu-Ping Shen et al. Relationship of plasma HIV-RNA levels and levels of TNF and immune activation products in HIV infection. Clinical Immunology and Immunopathology 1997 84 36– 45. 4 Van der Poll T, Romijn J, Endert E, Born JJ, Buller HR, Sauerwein HR et al. Tumor necrosis factor mimics the metabolic response to acute infection in healthy humans. American Journal of Physiology 1991 261 E457– E465. 5 Hotamisligil GS, Shargill NS & Spiegelman BM. Adipose expression of tumor necrosis factor-a: direct role in obesitylinked insulin resistance. Science 1993 259 87 –91. 6 Saghidazdeh M, Ong JM, Garvey WT, Henry RR & Kern PA. The expression of TNF by human muscle: relationship to insulin resistance. Journal of Clinical Investigation 1996 97 1111–1116. 7 Norman RA, Bogardus C & Ravussin E. Linkage between obesity and a marker near the tumor necrosis factor-alpha locus in Pima Indians. Journal of Clinical Investigation 1995 96 158– 162. 8 Uysal KT, Wiesbrock SM, Marino MW & Hotamisligil GS. Protection from obesity-induced insulin resistance in mice lacking TNFalpha function. Nature 1997 389 610– 614. 9 Bullo´ M, Garcı´a-Lorda P, Lo´pez-Soriano FJ, Argile´s JM & SalasSalvado´ J. Tumor necrosis factor, a key role in obesity? FEBS Letters 1999 451 215– 219. 10 Kern PA, Saghidazdeh M, Ong JM, Bosch RJ, Deem R & Simsolo RB. The expression of tumour necrosis factor in human adipose tissue: regulation by obesity, weight loss and relationship to lipoprotein lipase. Journal of Clinical Investigation 1995 95 2111–2119. 11 Hotamisligil GS, Arner PA, Caro JF, Atkinson RL & Spiegelman BM. Increased adipose tissue expression of tumor necrosis factor alpha in human obesity and insulin resistance. Journal of Clinical Investigation 1995 95 2409– 2415. 12 Corica F, Allegra A, Corsonello A, Buemi M, Calapai G, Ruello A et al. Relationship between plasma leptin levels and the tumor necrosis factor-system in obese subjects. International Journal of Obesity 1999 23 355 –360. 13 Hauner H, Bender M, Haastert B & Hube F. Plasma concentrations of soluble TNF-alpha receptors in obese subjects. International Journal of Obesity 1998 22 1239– 1243. 14 Aderka D, Engelmann HD, Maor Y, Brakebush C & Wallach D. Stabilization of the bioactivity of tumor necrosis factor by its soluble receptors. Journal of Experimental Medicine 1992 175 323– 329. 15 Hotamisligil GS, Arner P, Atkinson RL & Spiegelman BM. Differential regulation of the p80 tumor necrosis factor receptor in human obesity and insulin resistance. Diabetes 1997 46 451– 455. 16 Ro¨nnemaa T, Pulkki K & Kaprio J. Serum soluble tumor necrosis factor-a receptor 2 is elevated in obesity but is not related to insulin sensitivity: a study in identical twins discordant for obesity. Journal of Clinical Endocrinology and Metabolism 2000 85 2728–2732. 17 Ferna´ndez-Real JM, Broch M, Ricart W, Casamitjana R, Gutie´rrez C, Vendrell J et al. Plasma levels of the soluble fraction of tumor necrosis factor receptor 2 and insulin resistance. Diabetes 1998 47 1757–1762. 18 Chu NF, Spiegelman D, Rifai N, Hotamisligil GS & Rimm EB. Glycemic status and soluble tumor necrosis factor receptor levels in relation to plasma leptin concentrations among normal weight and overweight US men. International Journal of Obesity 2000 24 1085–1092.

TNFa – leptin system, adiposity and diabetes

331

19 Sociedad Espan˜ola para el Estudio de la Obesidad (SEEDO), Consenso SEEDO’2000 para la evaluacio´n de la obesidad y el establecimiento de criterios de intervencio´n terape´utica. Nutricio´n y Obesidad 2000 3 285– 299. 20 American Diabetes Association, Report of the Expert Committee on the diagnosis and classification of diabetes mellitus. Diabetes Care 1998 21 S5 –S19. 21 Segal K, Van Loan M, Fitzgerald PI, Hodgon JA & Van Itallie TB. Lean body mass estimation by bioelectrical impedance analysis: a four-site cross-validation study. American Journal of Clinical Nutrition 1998 7 7– 14. 22 Hube F, Birgel M, Lee YM & Hauner H. Expression pattern of tumor necrosis factor receptors in subcutaneous and omental human adipose tissue: role of obesity and non-insulin-dependent diabetes mellitus. European Journal of Clinical Investigation 1999 29 672–678. 23 Bemelmans MHA, Van Tis LJH & Buurman WA. Tumor necrosis factor: function, release and clearance. Critical Reviews in Immunology 1996 16 1–11. 24 Fried SK & Zechner R. Cachectin/tumor necrosis factor decreases human adipose tissue lipoprotein lipase mRNA levels, synthesis, and activity. Journal of Lipid Research 1989 30 1917–1923. 25 Semb H, Peterson J, Tavernier J & Olivecrona T. Multiple effects of tumor necrosis factor lipoprotein lipase in vivo. Journal of Biological Chemistry 1987 262 8390–8394. 26 Hotamisligil GS, Peraldi P, Budavari A, Ellis R, White MP & Spiegelman BM. IRS-1-mediated inhibition of insulin receptor tyrosine kinase activity in TNF- and obesity-induced insulin resistance. Science 1996 271 665–668. 27 Gasic S, Tian B & Green A. Tumor necrosis factor alpha stimulates lipolysis in adipocytes by decreasing Gi protein concentrations. Journal of Biological Chemistry 1999 274 6770–6775. 28 Mantzoros CS, Moschos S, Avramopoulos I, Kaklamani V, Liolios A, Doulgerakis DE et al. Leptin concentrations in relation to body mass index and the tumor necrosis factor-system in humans. Journal of Clinical Endocrinology and Metabolism 1997 82 3408–3413. 29 Considine RV, Sinha MK, Heiman ML, Kriauciunas A, Stephens TW, Nyce MR et al. Serum immunoreactive-leptin concentrations in normal-weight and obese humans. New England Journal of Medicine 1996 334 292– 295. 30 Socher SH, Friedman A & Martı´nez D. Recombinant human tumor necrosis factor induces acute reductions in food intake and body weight in mice. Journal of Experimental Medicine 1988 167 1957–1962. 31 Kirchgessner TG, Uysal KT, Wiesbrock SM, Marino MW & Hotamisligil GS. Tumor necrosis factor-alpha contributes to obesity-related hyperleptinemia by regulating leptin release from adipocytes. Journal of Clinical Investigation 1997 100 2777–2782. 32 Fawcett RL, Waetcher AS, Williams LLB, Zhang P, Louie R, Jones R et al. Tumor necrosis factor-a inhibits leptin production in subcutaneous and omental adipocytes from morbidly obese humans. Journal of Clinical Endocrinology and Metabolism 2000 85 530–535. 33 Zumbach MS, Boehme MWJ, Whal P, Stremmel W, Ziegler R & Nawroth PP. Tumor necrosis factor increases serum leptin levels in humans. Journal of Clinical Endocrinology and Metabolism 1997 82 4080–4082. 34 Medina EA, Stanhope KL, Mizuno TM, Mobbs CV, Gregoire F, Hubbard NE et al. Effects of tumor necrosis factor alpha on leptin secretion and gene expression: relationship to changes of glucose metabolism in isolated rat adipocytes. International Journal of Obesity 1999 23 896–903.

Received 11 July 2001 Accepted 3 December 2001

www.eje.org