polio replicon replication total replication total replication ... - PLOS

polio replicon replication

polio replicon replication

150000 100000

siControl FATP1 FATP3 FATP4 ACSL5

200000

50000

150000 100000

siControl FATP2 FATP5 FATP6 BG1

100000

50000

50000

0

0 12

14

16

0

2

4

hours post transfection

6

8

10

12

14

0

16

0

hours post transfection

total replication

100 80 60 40 20

140 120 100 80 60 40 20

G

2

0

140 100 80 60 40 20

1

40

si C on tr ol

SL 5 A C

TP 4 FA

TP 3 FA

TP 1 FA

B

6 C SL A

C SL A

C SL

si C on tr ol

0

G 2

0 4

20

0

3

20

1

20

A

B G

60

40

C SL

TP 6

80

1

60

100

B G

80

40

A

FA

si C on tr ol

SL 5 A C

TP 4 FA

TP 3 FA

TP 1

120

100

TP 6

60

140

120

FA

80

140

TP 2

100

160

FA

120

toxicity

160

% of control

% of control

140

on tr ol

14

0

FA

si C on tr ol

A

160

% of control

12

120

toxicity toxicity

si C

10

B

C

SL 6

SL 4 C

A

A

C

SL 3

SL 1 C A

si Co nt r

ol

0

8

160

% of control replication

% of control replication

120

6

total replication

160

140

4

hours post transfection

total replication

160

2

TP 5

10

FA

8

TP 5

6

FA

4

TP 2

2

FA

0

% of control replication

150000

renilla RLU

siControl ACSL1 ACSL3 ACSL4 ACSL6 BG2

polio replicon replication

250000

renilla RLU

renilla RLU

200000

16