state reactivity probe, 2,2'-dipyridyl disulphide, of partially purified and fully purified sampies of stem bromelain (EC. 3.4.22.4) reported by Brocklehurst et al.
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muscle per min. This value takes into consideration the different concentrations of AIB present in the muscle after preloading, since loading in the presence of cortisol inevitably yields a lower value than that of the control. In addition, a correction to efAux has to be made for the initial release of AIB from the extracellular space. From experiments involving the use of muscles preloaded with the extracellular-space marker [I4C]inulin, it was found that, within Smin, 65% of more of the inulin had been washed out. Hence 5min of incubation was taken as the zero time for measuring the intracellular efAux of AIB. The net accumulation of AIB was significantly inhibited (-23%, P < 0.001) after 4 h exposure to lpwcortisol, but not
lower concentrations or shorter times. This generally agrees with Kostyo (1965), who assumed that such an inhibition of net transfer occurred through a depression of influx into the muscle. However, our studies indicate that the influx of AIB is not affected by the steroid (+8%; P
