atic study on the effect of secondary structure on the ability of oligonucleotide probes to detect an SNP, using real-time array mon- itoring of a porous microarray ...
Product Application Focus Effect of Secondary Structure on Single Nucleotide Polymorphism Detection with a Porous Microarray Matrix; Implications for Probe Selection R.M. Anthony, A.R.J. Schuitema, A.B. Chan1, P.J. Boender1, P.R. Klatser, and L. Oskam KIT Biomedical Research, Amsterdam, and 1PamGene International BV, ’s-Hertogenbosch, The Netherlands BioTechniques 34:1082-1089 (May 2003)
DRUG DISCOVERY AND GENOMIC TECHNOLOGIES
ABSTRACT Oligonucleotide arrays capable of detecting single nucleotide polymorphisms (SNPs) from amplified nucleic acid have many applications. The expected SNP is usually placed approximately in the center of the probe to ensure the maximum shift in Tm between complementary and SNP sequences. Unfortunately, different short probes (
