Mark A. Vickers,1,2* and Robert N. Barker1,*. 1Division of Applied .... of Eagles medium (α-MEM; Gibco/Invitrogen, Paisley, UK) supple- mented with 1% 2 mM ...
Articles and Brief Reports
Red Cell Disorders
Production of the effector cytokine interleukin-17, rather than interferon-γ, is more strongly associated with autoimmune hemolytic anemia Andrew M. Hall,1 Omar M. Zamzami,1 Natasha Whibley,1 Daniel P. Hampsey,1 Anne M. Haggart,1 Mark A. Vickers,1,2* and Robert N. Barker1,* 1 Division of Applied Medicine, University of Aberdeen, UK; 2Academic Transfusion Medicine Unit, Scottish National Blood Transfusion Service, UK
ABSTRACT *Joint senior authors Background Interleukin-17A is the signature cytokine of the Th17 subset and drives inflammatory pathology, but its relevance to autoantibody-mediated diseases is unclear. Th1 cells secreting interferon-γ have been implicated in autoimmune hemolytic anemia, so the aim was to determine which cytokine is more closely associated with disease severity.
Design and Methods Interferon-γ and interleukin-17A were measured in the sera of patients with autoimmune hemolytic anemia and healthy donors, and in peripheral blood mononuclear cell cultures stimulated with autologous red blood cells, or a panel of peptides spanning red blood cell autoantigen.
Results Serum interleukin-17A, but not interferon-γ, was significantly raised in patients with autoimmune hemolytic anemia (P2) to RBC autoantigen were commoner in patients with more severe anemia (n=7) than in those with low-grade hemolysis (n=7) or in healthy donors (n=7), the differences in cytokine production
2.5
16
2.0 12
IL-17 (pg/mL)
The non-parametric Mann-Whitney U test and Spearman’s rank correlation were performed utilizing Minitab 15 software (Minitab Ltd., Coventry, UK), with the level for statistical significance taken as P
