Progress toward Ultrafast DNA Sequencing Using Solid ... - CiteSeerX
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Progress toward Ultrafast DNA Sequencing Using Solid ... - CiteSeerX
ing through nanometer-scale pores (nanopores) have been used to analyze single ... DNA translocating unzipping through a nanopore. Our technique offers ...
Clinical Chemistry 53:11 1996 –2001 (2007)
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Progress toward Ultrafast DNA Sequencing Using Solid-State Nanopores Gautam V. Soni and Amit Meller* Background: Measurements of the ionic current flowing through nanometer-scale pores (nanopores) have been used to analyze single DNA and RNA molecules, with the ultimate goal of achieving ultrafast DNA sequencing. However, attempts at purely electronic measurements have not achieved the signal contrast required for single nucleotide differentiation. In this report we propose a novel method of optical detection of DNA sequence translocating through a nanopore. Methods: Each base of the target DNA sequence is 1st mapped onto a 2-unit code, 2 10-bp nucleotide sequence, by biochemical conversion into Designed DNA Polymers. These 2-unit codes are then hybridized to complementary, fluorescently labeled, and self-quenching molecular beacons. As the molecular beacons are sequentially unzipped during translocation through a
