Project Overview Module 1: Resource-full Waste Module ... - iGEM 2013

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vector. Substrate alone. Poly-3-hydroxybutyrate synthesis from waste. Improved Poly-3-hydroxybutyrate production ... Featured on BBC radio 4 Inside Science.
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Project Overview

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M1: Resource-full Waste

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Module 1: Resource-full Waste

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to

Treasure

Module 2: Plastic Fantastic

Non-recyclable, mixed waste bdh2

Degradation of non-recyclable, mixed waste into platform chemicals and feedstock for Poly-3-hydroxybutyrate P(3HB) production.

Polyurethane degradation

Ethylene Glycol sold into industry

Wood, paper & fibres

Polyurethane degradation

Poly-3-hydroxybutyrate degradation

Functional Polyurethane (PUR) esterase with substrate analogue

P(3HB) depolymerase (phaZ1) degrades P(3HB) to produce a distinct clear zone in the emulsion plate

M2: Plastic Fantastic The first synthetic biology P(3HB) recycling platform.

Control

PUR esterase in the supernatant

Empty vector

phaZ1 lysate

P(3HB) degradation

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PUR EstCS2

P(3HB) production

Human Practices & Outreach

Substrate alone

Western Blot analysis suggests that the osmY fusion protein is secreted

P(3HB) depolymerase (phaZ1) degrades P(3HB) into 3HB

Poly-3-hydroxybutyrate synthesis from waste

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SRF waste from POWERDAY used as a carbon source for P(3HB) production

• We consulted industrial leaders in Materials Recovery, Bioplastic Production and Biotechnology

Yellow colour change when 3HB is present

Control. Remains colourless

Monomer detection

Improved Poly-3-hydroxybutyrate production • We held a Panel Discussion with a variety of academics and experts to talk about the societal, ethical and technological challenges surrounding our project. • With artists from the RCA, we have designed a range of futuristic appliances, M.A.P.L.E., to empower people to use their own rubbish as a resource.

Our model indicated that J23104 would improve P(3HB) production

Native Hybrid

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Separation of 3HB via Membranes 100% 90% 80% 70% 60% 50% 40%

94%

84%

DuraMem150

PBI-DBX

95%

30% 20% 10% 0%

TriSep RO X20

Preliminary data in collaboration with Professor Livingstone group, Chemical Engineering at Imperial College London

Team & Attributions NEW TEAM PHOTOS GO HERE

We subsequently designed several new Biobricks

Sisi Fan, Jemma Pilcher, Iain Bower, Matthew Chin, Margarita Kopniczky, Wenqiang Chi & James Strutt

Constitutive Promoter, BBa_K1149052

Communicating our project Featured on BBC radio 4 Inside Science We founded an art movement BioNouveau Presentation for Celebration of Science Online quiz 5 Waste Offenders recycling posters What would you 3D print? survey

Industrial Implementation MG1655 transformed with our Hybrid phaCAB bioplastic producing operon were cultured in a bioreactor at Imperial College London

How could our system be implemented in real life?

• • • • • •

Increased 3HB concentration detected

J23104

0034

pha C

pha A

pha B

Hybrid Promoter, BBa_K1149051

J23104

0034

Native

pha C

pha A

Europe’s Best New BioBrick Part or Device, Engineered

pha B

Our improved Biobrick makes 11 x more P(3HB)

Collaborators from the Royal College of Art Marcel Helmer, Alexa Pollmann & Neil Thompson Many thanks to our advisors Richard Kelwick, Paul Freemount, Richard Kitney, Alex Webb, Kirsten Jensen, Guy-Bart Stan and the CSynBI team Many thanks to our sponsors