vector. Substrate alone. Poly-3-hydroxybutyrate synthesis from waste. Improved Poly-3-hydroxybutyrate production ... Featured on BBC radio 4 Inside Science.
Trash
Project Overview
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M1: Resource-full Waste
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Module 1: Resource-full Waste
4
to
Treasure
Module 2: Plastic Fantastic
Non-recyclable, mixed waste bdh2
Degradation of non-recyclable, mixed waste into platform chemicals and feedstock for Poly-3-hydroxybutyrate P(3HB) production.
Polyurethane degradation
Ethylene Glycol sold into industry
Wood, paper & fibres
Polyurethane degradation
Poly-3-hydroxybutyrate degradation
Functional Polyurethane (PUR) esterase with substrate analogue
P(3HB) depolymerase (phaZ1) degrades P(3HB) to produce a distinct clear zone in the emulsion plate
M2: Plastic Fantastic The first synthetic biology P(3HB) recycling platform.
Control
PUR esterase in the supernatant
Empty vector
phaZ1 lysate
P(3HB) degradation
2
PUR EstCS2
P(3HB) production
Human Practices & Outreach
Substrate alone
Western Blot analysis suggests that the osmY fusion protein is secreted
P(3HB) depolymerase (phaZ1) degrades P(3HB) into 3HB
Poly-3-hydroxybutyrate synthesis from waste
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SRF waste from POWERDAY used as a carbon source for P(3HB) production
• We consulted industrial leaders in Materials Recovery, Bioplastic Production and Biotechnology
Yellow colour change when 3HB is present
Control. Remains colourless
Monomer detection
Improved Poly-3-hydroxybutyrate production • We held a Panel Discussion with a variety of academics and experts to talk about the societal, ethical and technological challenges surrounding our project. • With artists from the RCA, we have designed a range of futuristic appliances, M.A.P.L.E., to empower people to use their own rubbish as a resource.
Our model indicated that J23104 would improve P(3HB) production
Native Hybrid
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Separation of 3HB via Membranes 100% 90% 80% 70% 60% 50% 40%
94%
84%
DuraMem150
PBI-DBX
95%
30% 20% 10% 0%
TriSep RO X20
Preliminary data in collaboration with Professor Livingstone group, Chemical Engineering at Imperial College London
Team & Attributions NEW TEAM PHOTOS GO HERE
We subsequently designed several new Biobricks
Sisi Fan, Jemma Pilcher, Iain Bower, Matthew Chin, Margarita Kopniczky, Wenqiang Chi & James Strutt
Constitutive Promoter, BBa_K1149052
Communicating our project Featured on BBC radio 4 Inside Science We founded an art movement BioNouveau Presentation for Celebration of Science Online quiz 5 Waste Offenders recycling posters What would you 3D print? survey
Industrial Implementation MG1655 transformed with our Hybrid phaCAB bioplastic producing operon were cultured in a bioreactor at Imperial College London
How could our system be implemented in real life?
• • • • • •
Increased 3HB concentration detected
J23104
0034
pha C
pha A
pha B
Hybrid Promoter, BBa_K1149051
J23104
0034
Native
pha C
pha A
Europe’s Best New BioBrick Part or Device, Engineered
pha B
Our improved Biobrick makes 11 x more P(3HB)
Collaborators from the Royal College of Art Marcel Helmer, Alexa Pollmann & Neil Thompson Many thanks to our advisors Richard Kelwick, Paul Freemount, Richard Kitney, Alex Webb, Kirsten Jensen, Guy-Bart Stan and the CSynBI team Many thanks to our sponsors
