Oct 22, 2014 - ICSI, affect fertilisation outcome, embryo quality and morphokinetic ... (7%vs6%), 3PN (2%vs1%), proportion of embryos with even first cell divi-.
CONCLUSION: In good responders, triggering ovulation less than 4 days from an estradiol level of 400 pg/mL leads to higher blastulation and implantation rates and likely higher clinical pregnancy and live birth rates. Further studies are needed to determine whether these findings hold true in other patient cohorts. P-558 Wednesday, October 22, 2014 PROSPECTIVE VALIDATION OF A TIME-LAPSE BASED ALGORITHM FOR EMBRYO SELECTION. S. Perez, I. Rubio, B. Aparicio, D. Beltran, V. Garcıa-Laez, M. Meseguer. IVF Laboratory, Instituto Universitario IVI Valencia, University of Valencia, Valencia, Spain. OBJECTIVE: To validate a published morphokinetic algorithm (Meseguer et al., 2011) for embryo selection by time-lapse technology. DESIGN: Prospective, randomized, triple blinded, controlled study. This study includes 930 patients undergoing IVF. MATERIALS AND METHODS: Patients were randomly divided into a control group (patients whose embryos developed in a conventional incubator (SI) and were assessed only by conventional morphological criteria) and a study group (in which embryos were cultured in the time-lapse monitoring system (TMS) EmbryoScope (Fertiiltech, Aahrus, DK) and were evaluated using a hierarchical morphokinetic model). The morphokinetic model establishes five embryo categories based on precise timing of cell division. Embryo implantation rate (IR) was evaluated in each morphokinetic category in the TMS group or morphological criteria in the SI group. RESULTS: We observed a direct relationship between morphokinetic categories and implantation potential in TMS group. Also a direct relationship was observed between morphology categories and implantation rates in SI.
MATERIALS AND METHODS: For objectives (a) and (b), 160 mature oocytes derived from 22 patients were injected by two practitioners. Oocytes from each patient were randomly split to be injected either using the I3 (n¼ 82) or the IntegraTI (n¼78) systems. For objective (c), two technicians (not the practioners) independently assessed the videos of 108 individual ICSIs from 17 patients with an average age of 39.4 years and average cycle number of 1.8 for four individual techniques: injection symmetry, oolemma breakage, furrow persistance and oolemma breakage. Injected oocytes were cultured in an EmbryoscopeÒ to assess PIs and morphokinetics. Continuous data (morphokinetics) were analysed using a generalised linear mixed model (assessed parameter¼ICSI rig*practitioner), with patient as the random factor. Categorical data (Performance Indicators, PIs) were assessed using Chi-square test and considered significant where p
