The efficacy of DNA vaccines encoding the duck hepatitis B virus (DHBV) pre-S/S and S proteins were tested in Pekin ducks. Plasmid pcDNA I/Amp DNA ...
JOURNAL OF VIROLOGY, Jan. 1998, p. 84–94 0022-538X/98/$04.0010 Copyright © 1998, American Society for Microbiology
Vol. 72, No. 1
Protective Efficacy of DNA Vaccines against Duck Hepatitis B Virus Infection M. TRIYATNI,1* A. R. JILBERT,1,2 M. QIAO,2 D. S. MILLER,1 1,2 AND C. J. BURRELL Department of Microbiology and Immunology, University of Adelaide, Adelaide, South Australia 5005,1 and Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Adelaide, South Australia 5000,2 Australia Received 12 May 1997/Accepted 18 September 1997
The efficacy of DNA vaccines encoding the duck hepatitis B virus (DHBV) pre-S/S and S proteins were tested in Pekin ducks. Plasmid pcDNA I/Amp DNA containing the DHBV pre-S/S or S genes was injected intramuscularly three times, at 3-week intervals. All pre-S/S and S-vaccinated ducks developed total anti-DHBs and specific anti-S antibodies with similar titers reaching 1/10,000 to 1/50,000 and 1/2,500 to 1/4,000, respectively, after the third vaccination. However, following virus challenge, significant differences in the rate of virus removal from the bloodstream and the presence of virus replication in the liver were found between the groups. In three of four S-vaccinated ducks, 90% of the inoculum was removed between
