protein endopeptidase Observed theoretical residue number ... - PLOS

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Chymotrypsin. 698.19. 1+. 698.19. 698.32. 1-6. LC-MS. Chymotrypsin. 862.36. 2+. 1723.72. 1723.73. 37-39, 84-94. Cys2 – 5. LC-MS. V8 endopeptidase.
protein Dmel\OBP57d

Dmel\OBP57e

Dpse\OBP57d

endopeptidase trypsin trypsin Chymotrypsin Chymotrypsin V8 endopeptidase V8 endopeptidase Chymotrypsin V8 endopeptidase Chymotrypsin V8 endopeptidase

m/z 7281.02 686.30 698.19 862.36 2722.74 916.70 816.33 823.86 743.72 3674.23

Observed charge 1+ 2+ 1+ 2+ 1+ 3+ 2+ 2+ 2+ 1+

mass 7281.02 1371.60 698.19 1723.72 2722.74 2748.10 1631.66 1646.72 1486.44 3674.23

theoretical mass 7281.16 1371.70 698.32 1723.73 2721.38 2748.41 1631.74 1646.87 1486.52 3675.19

residue number 1-33, 34-56, 59-66 57-58, 67-75 1-6 37-39, 84-94 53-75 76-82, 92-108 9-16, 46-51 22-34 43-45, 89-97 75-87, 97-115

S-S Pair Cys1- 2- 3- 5 Cys4 - 6 Cys2 – 5 Cys4 – 6 Cys1 – 3 Cys2 – 5 Cys4 – 6

instrument MALDI LC-MS LC-MS LC-MS MALDI LC-MS LC-MS LC-MS LC-MS MALDI