Quantitation of Porcine Cytomegalovirus in Pig Tissues by PCR

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Corresponding author. Mailing address: Department of Virology,. Royal Free and University College Medical School, Royal Free Cam- pus, Rowland Hill Street, ...
JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 2001, p. 1155–1156 0095-1137/01/$04.00⫹0 DOI: 10.1128/JCM.39.3.1155–1156.2001 Copyright © 2001, American Society for Microbiology. All Rights Reserved.

Vol. 39, No. 3

Quantitation of Porcine Cytomegalovirus in Pig Tissues by PCR JACQUELINE F. L. FRYER,1 PAUL D. GRIFFITHS,1 JAY A. FISHMAN,2 VINCENT C. EMERY,1 1 AND DUNCAN A. CLARK * Department of Virology, Royal Free and University College Medical School of UCL, London, United Kingdom,1 and Transplant Infectious Disease Program, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts2 Received 5 September 2000/Returned for modification 27 November 2000/Accepted 21 December 2000

A quantitative-competitive PCR for the quantification of porcine cytomegalovirus (PCMV) was developed. The virus was detected in a variety of pig organs (including potential xenotransplant donations), with viral loads ranging from