Corresponding author. Mailing address: Department of Virology,. Royal Free and University College Medical School, Royal Free Cam- pus, Rowland Hill Street, ...
Quantitation of Porcine Cytomegalovirus in Pig Tissues by PCR JACQUELINE F. L. FRYER,1 PAUL D. GRIFFITHS,1 JAY A. FISHMAN,2 VINCENT C. EMERY,1 1 AND DUNCAN A. CLARK * Department of Virology, Royal Free and University College Medical School of UCL, London, United Kingdom,1 and Transplant Infectious Disease Program, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts2 Received 5 September 2000/Returned for modification 27 November 2000/Accepted 21 December 2000
A quantitative-competitive PCR for the quantification of porcine cytomegalovirus (PCMV) was developed. The virus was detected in a variety of pig organs (including potential xenotransplant donations), with viral loads ranging from