Jul 20, 2017 - amperometry (FPA) method using specific electrode to measure adenosine ... in blood using fixed potential amperometry (FAP) and to compare.
Journal of
ech al T nique tic
Analytical & Bioanalytical Techniques
s
of rnal Analy ou
Bioan aly al & tic
J
ISSN: 2155-9872
Research Article
Marlinge et al., J Anal Bioanal Tech 2017, 8:4 DOI: 10.4172/2155-9872.1000371
OMICS International
Rapid Measurement of Adenosine Concentration in Human Blood Using Fixed Potential Amperometry: Comparison with Mass Spectrometry and HighPerformance Liquid Chromatography Marion Marlinge1, Donato Vairo2, Viviana Marolda2, Laurie Bruzzese2, Nabil Adjriou2, Claire Guiol2, Nathalie Kipson2, Anna Bonnardel2, Marguerite Gastaldi1, François Kerbaul3, Pierre Michelet3, Jean Claude Deharo4, Giovanna Mottola2, Patrick Mace1, Mohamed Chefrour1 and Regis Guieu1,2* Laboratory of Biochemistry, Timone Hospital 264 Rue Saint-Pierre, 13385 Marseille, France UMR MD2, Aix Marseille University, Bd Pierre Dramard, 13015 Marseille, France 3 RUSH Department, Timone 2, 264 Rue Saint-Pierre, 13385 Marseille, France 4 Department of Cardiology, Timone Hospital 264 Rue Saint-Pierre, 13385 Marseille, France 1 2
Abstract Background: Adenosine is a nucleoside that impacts the cardiovascular system during cardiovascular or inflammatory diseases. The rapid determination of adenosine in blood may be useful in emergency medicine especially in syncope diagnose or septic shock. We compare its measurement in blood using fixed potential amperometry (FPA), with usual methods: mass spectrometry (LC-MS/MS) or high performance liquid chromatography (HPLC). Methods: Twenty healthy subjects (14 men and 6 women) and ten patients suffering from vasovagal syncope (VVS, 6 women and 4 men) were included. Blood samples were collected by vein puncture for plasma adenosine assay and in the same time using finger puncture for direct FAP measurement and on blotting paper for LC-MS/MS. Results: Mean plasma adenosine concentration was 26% higher using HPLC compared with LC-MSMS; p
