Rapid micro fluorescence in situ hybridization in ... - AIP Publishing
Recommend Documents
hypotonic treatment and fixation of direct demecolcine treated cultures, followed by dehydration, codenaturation, overnight hybridization, washing and mounting ...
some instances the correct interpretation on the nature of these changes may be ... analysis by rapidly and unambiguously delin- ... occasional blood test displaying a moderate .... Bartram CR, Anger B, Carbonell F, Kleihauer E . Involvement.
have X homologues escaping X inactivation and the second consisting of Y-chromosomal ... the `®bre-FISH' technique as a complementary mapping strategy.
Bong-Kyu C h ~ i , ~ Michael Wagner5 and Ulf B. Gobel'. Author for correspondence: Ulf B. Gobel. ... (Blowey et al., 1994) or silver staining (Elias & Bosma,.
Feb 10, 2014 - International Journal of Cytology, Cytosystematics and Cytogenetics. ISSN: 0008-7114 (Print) ... 63 - Number 3. Downloaded by [182.253.143.7] at 19:22 30 January 2016 .... did not remain free of hybridization signals. DISCUSSION .... G
patterns of RNA transcription that play roles in cell function, ... ization with labeled probes in order to detect DNA and/or. RNA sequences ... Invited Mini Review ...
Sign in. Loading⦠Whoops! There was a problem loading more pages. Retrying... Whoops! There was a problem previewing t
Feb 5, 2014 - and ETS family members (ERG, RTV1, ETV4) were detected in nearly half of the prostate cancers but none of the benign prostate tissues that ...
NContext.âGene expression profiling of diffuse large B- cell lymphoma using complementary DNA microarrays has revealed 2 major prognostic groups in ...
Jan 27, 2010 - the classes Deltaproteobacteria (5.8 to 28.3%) and Gammaproteobacteria (1.2 to 8.2%). All were more .... sharpened in Adobe Photoshop CS3.
Oct 20, 1993 - on metaphase chromosomes and interphase Halo-preparations using cosmid and YAC clones for the simultaneous high resolution mapping.
Abstract. Urothelial carcinomas arising from the upper urinary tract (renal pelvis and ureter) are rare and few molecular genetic studies of these tumors have.
the location of several critical genes in prostate can- cer. For example, initial evidence of nonrandom prostate cancer chromosome aberrations included.
Jan 24, 2013 - Pablo Martinez1*, Javier Herna´ndez-Losa2, Ma A´ ngeles Montero2, Susana Cedre´s1, Josep Castellvı2,. Alex Martinez-Marti1, Natalia ...
Mar 29, 2018 - ologic and aberrant myeloblast populations in the day-14 aspirate presents a prognostic dilemma [5]. Highly sensitive diagnostic methods for ...
&p.1:Abstract We have used horseradish peroxidase-labeled. 40 mer oligodeoxynucleotides (HRP-ODNs) specific for the human cytomegalovirus immediate ...
May 3, 2017 - independent experiments, n = 10 or 11 cells per experiment. ACS Central Science. Research Article. DOI: 10.1021/acscentsci.7b00048.
Photomicrographs were taken using a Kodak. Ektachrome HC 400 film. A Zeiss confocal laser scanning micro- scope equipped with an Ar-ion laser (488 nm) ...
May 30, 2018 - ferred to an ethanol-containing (Sigma Aldrich, Cat. ..... Hammond, D. G. Hicks, M. Dowsett, L. M. McShane, K. H. Allison, D. C. Allred, J. M. S..
BIOMICROFLUIDICS 12, 042212 (2018)
Rapid micro fluorescence in situ hybridization in tissue sections D. Huber and G. V. Kaigalaa) IBM Research – Z€ urich, S€ aumerstrasse 4, 8803 R€ uschlikon, Switzerland (Received 28 January 2018; accepted 29 March 2018; published online 30 May 2018)
This paper describes a micro fluorescence in situ hybridization (lFISH)-based rapid detection of cytogenetic biomarkers on formalin-fixed paraffin embedded (FFPE) tissue sections. We demonstrated this method in the context of detecting human epidermal growth factor 2 (HER2) in breast tissue sections. This method uses a non-contact microfluidic scanning probe (MFP), which localizes FISH probes at the micrometer length-scale to selected cells of the tissue section. The scanning ability of the MFP allows for a versatile implementation of FISH on tissue sections. We demonstrated the use of oligonucleotide FISH probes in ethylene carbonate-based buffer enabling rapid hybridization within
