Rapid micro fluorescence in situ hybridization in ... - AIP Publishing
Recommend Documents
May 3, 2017 - independent experiments, n = 10 or 11 cells per experiment. ACS Central Science. Research Article. DOI: 10.1021/acscentsci.7b00048.
the location of several critical genes in prostate can- cer. For example, initial evidence of nonrandom prostate cancer chromosome aberrations included.
No. of courses. Radiotherapy. Dose. (Grays). Time between treatment and. FISH analysis. Lymphoma. L1*. 27. HD. ABVD. 3. Retroperitoneal and splenic. 30.
extracted nuclei from snap frozen buffy coat stored for the purpose of molecular ... by lysing erythrocytes with lysing solution (LYSE S III DIFF, Coulter Corp.).
Sep 2, 2015 - in the paper and S1 & S2 Tables. Funding: This study was supported by NIH SBIR. Phase I and Phase II Grants # A1056785. ID-FISH.
Specific image analysis programs were .... hybridization protocol and the best instrumentation setup ... Filter Sets Used for Multiple Fluorescence ISH Analysis".
Department of Medicine, Mayo Clinic College of Medicine, Rochester, MN. Key Words: .... ITable 1I. List of AML FISH Probes and Targets. Targeted Abnormality.
probe sequence segments, i.e., POL, MAC, P-M, and pan-16S, used in this study are also indicated. kindly provided) by Gary Olsen of the University of Illinois.
mosomes appear fluffy after hybridization (Fig. 1 Right). With increased .... Graham, G. J., Hall, T. J. & Cummings, M. R. (1984) Am. J. Hum. Genet. 36, 25-35. 11.
appropriate filter sets for red, green and blue fluorescence (8) was used. A 63xlens with a ..... Cooke,H.J., Hindley,J. (1979) Nucl. Acids Res. 6, 3177-3197. 19.
Rapid micro fluorescence in situ hybridization in ... - AIP Publishing
May 30, 2018 - ferred to an ethanol-containing (Sigma Aldrich, Cat. ..... Hammond, D. G. Hicks, M. Dowsett, L. M. McShane, K. H. Allison, D. C. Allred, J. M. S..
BIOMICROFLUIDICS 12, 042212 (2018)
Rapid micro fluorescence in situ hybridization in tissue sections D. Huber and G. V. Kaigalaa) IBM Research – Z€ urich, S€ aumerstrasse 4, 8803 R€ uschlikon, Switzerland (Received 28 January 2018; accepted 29 March 2018; published online 30 May 2018)
This paper describes a micro fluorescence in situ hybridization (lFISH)-based rapid detection of cytogenetic biomarkers on formalin-fixed paraffin embedded (FFPE) tissue sections. We demonstrated this method in the context of detecting human epidermal growth factor 2 (HER2) in breast tissue sections. This method uses a non-contact microfluidic scanning probe (MFP), which localizes FISH probes at the micrometer length-scale to selected cells of the tissue section. The scanning ability of the MFP allows for a versatile implementation of FISH on tissue sections. We demonstrated the use of oligonucleotide FISH probes in ethylene carbonate-based buffer enabling rapid hybridization within
