Rat Housekeeping Genes PCR Array

Rat Housekeeping Genes PCR Array

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Housekeeping Genes PCR Array NOTE: To access content of RT² Profiler PCR Arrays (Prior to May 25, 2012), please click here. Please NOTE: The RT² Profiler PCR Array System was upgraded to Version 4.0 on May 25, 2012. This system has replaced the Version 3.0 system with regards to content and assay design, with the Version 3.0 products set for discontinuation effective December 1, 2012. Human

Browse by Pathway Apoptosis Biomarkers Cell Cycle Cytokine & Inflammation ECM & Adhesion Neuroscience Signal Transduction Stem Cell & Development

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Rat Housekeeping Genes PCR Array The Rat Housekeeping Genes RT² Profiler™ PCR Array profiles the expression of eight (8) identical sets of twelve (12) commonly used housekeeping genes. This array can be easily used to identify genes with a constant level of expression among your different experimental conditions for use in normalizing your relative gene expression profiling experiment. Housekeeping genes encode proteins that are usually essential for the maintenance of cellular function. Their expression often, but not always, remains constant under most experimental conditions. Some of these genes may be regulated under certain circumstances and may vary with cell type. Using real-time PCR, you can easily and reliably evaluate the expression of these housekeeping genes among your different experiment conditions with this array.

Toxicology & Drug ADME Protocol Guide

96-well Plate, 384-well (4 × 96) Plate, and 100-well Disc formats are available. Available for cells, tissues, FFPE samples and small samples

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Gene Table

Infectious Diseases Metabolic Diseases Column

UniGene

GenBank

Symbol

Description

Browse by Epigenetics 1

Rn.94978

NM_031144

Actb

Actin, beta

2

Rn.1868

NM_012512

B2m

Beta-2 microglobulin

4

Rn.47

NM_012583

Hprt1

Hypoxanthine phosphoribosyltransferase 1

Rn.98667

NM_001004082

Hsp90ab1

Heat shock protein 90 alpha (cytosolic), class B member 1

5

Rn.107896

NM_017025

Ldha

Lactate dehydrogenase A

6

Rn.8381

NM_001012356

Nono

Non-POU domain containing, octamer-binding

7

Rn.108127

NM_053291

Pgk1

Phosphoglycerate kinase 1

8

Rn.129133

XM_345576

Ppih

Peptidylprolyl isomerase H (cyclophilin H)

9

Rn.973

NM_001007604

Rplp1

Ribosomal protein, large, P1

Rn.101725

NM_130428

Sdha

Succinate dehydrogenase complex, subunit A, flavoprotein (Fp)

3

10

http://www.sabiosciences.com/...430+Real-Time+PCR%3A+House+Keeping+Genes_us&utm_campaign=PR130430+Housekeeping+Genes&utm_source=iPost&utm_medium=email[30/04/2013 1:16:47 PM]

Rat Housekeeping Genes PCR Array 11

Rn.22712

NM_001004198

Tbp

TATA box binding protein

12

Rn.98672

NM_022712

Tfrc

Transferrin receptor Gene Table


How It Works

Manual & Resources

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"If you have access to a real-time PCR instrument, you can use PCR Arrays" RT² Profiler™ PCR Arrays are the most reliable and sensitive gene expression profiling technology for analyzing a panel of genes in signal transduction pathways, biological process or disease related gene networks. The PCR Arrays can be used for research on cancer, immunology, stem cells, toxicology, biomarker discovery and validation, and phenotypic analysis of cells and transgenic animals. See the complete list of PCR Arrays. You can use any 96-well or 384-well real-time PCR instrument. See instrument-specific setup instructions. Click below for detailed information. How it Works

Performance Data

Application Data

How it Works The PCR array is a set of optimized real-time PCR primer assays on 96-well or 384-well plates for pathway or disease focused genes as well as appropriate RNA quality controls. The PCR array performs gene expression analysis with real-time PCR sensitivity and the multi-gene profiling capability of a microarray. Simply mix your cDNA template with the appropriate ready-to-use PCR master mix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program. (Download user manual)

Figure 1:

How PCR Arrays Work - Protocol Chart

What it offers? Guaranteed Performance* - ready-to-use for gene expression analysis Time and cost saving - less than 30 min hands-on time for analyzing 84 genes Ease of data analysis using our easy-to-use Excel-based data analysis template or web-based analysis tool Layout and Controls: The PCR Arrays are available in both 96- and 384-well plates and are used to monitor the expression of 84 genes related to a disease state or pathway plus five housekeeping genes. Controls are also included on each array for genomic DNA contamination, RNA quality, and general PCR performance


Rat Housekeeping Genes PCR Array You can easily perform a PCR Array experiment in your own laboratory, or send your samples to us and take advantage of our PCR Array Services. *: when using complete PCR array system. Performance Data Sensitivity: The complete PCR Array System yields a greater-than 85 percent present call with as little as 25 ng as much as 5 µg of total RNA from a pathway representing genes expressed at a lower level (inflammatory cytokines and receptors).

Figure 2:

PCR Arrays Let You See More Genes with Less RNA Different amounts of universal total RNA were characterized using the Human Inflammatory Cytokines and Receptors PCR Array, and the percentage of detectable genes was calculated for each RNA amount. As little as 25 ng total RNA yields greater than an 80% positive call, even for cytokines expressed at very low levels.

Reproducibility The complete PCR Array System demonstrates strong correlations across technical replicates, lots, and instruments with average correlation coefficients > 0.99 insuring reliable detection of differences in expression between biological samples.

060111_1 060111_2 060111_3 060111_4 Figure 3:

050825_1 0.993 0.994 0.992 0.993

050825_2 0.989 0.990 0.990 0.992

050825_3 0.995 0.995 0.993 0.994

050825_4 0.992 0.992 0.992 0.992

PCR Arrays Yield Highly Reproducible Results Four replicate sets of raw threshold data (1-4) obtained by two different scientists (A & B) at two different times (050825 & 060111) on Human Drug Metabolism RT² Profiler PCR Arrays are directly compared. The results demonstrate a high degree of correlation (R 2 > 0.990).

Specificity The complete PCR Array System, with high quality input RNA, is guaranteed to yield single bands of the predicted size without primer dimers or other secondary products thus providing the most accurate real-time PCR results possible.



Figure 4:

PCR Arrays Amplify A Single Gene-Specific Product in Every Reaction. Universal total RNA was characterized on the TGFβ / BMP Signaling Pathway PCR Array, followed by dissociation (melt) curve analysis. PCR Arrays specifically detect individual genes despite the expression of related gene family members in the same RNA sample.

Application Data Cancer Research: To ascertain the oncogenic route that two different human breast tumors have taken, the relative expression level of cancerand adhesion-related genes in normal and two different cancerous tissues were compared. Template cDNAs prepared from total RNA of normal human breast and two human breast tumors (BioChain Institute, Inc., 5.0 µg) were characterized in technical triplicates using the Human Cancer PathwayFinder™ PCR Array and the Human Extracellular Matrix & Adhesion Molecule PCR Array with the RT² SYBR Green / Fluorescein PCR master mix on the Bio-Rad iCycler®.

Figure 5:

ECM & Adhesion PCR Arrays Revealed Up- and Down-Regulated Genes in Breast Cancer Total RNA from normal human breast and a human breast tumor were characterized in technical triplicates, and the relative expression levels for each gene in the two samples are plotted against each other in the Scatter Plot. Genes encoding the matrix metallopeptidases (MMP3 & MMP9) and their inhibitors (TIMP3) are up-regulated, while genes encoding integrins (ITGB3 & ITGB4) are down-regulated, by at least three-fold (outside the silver field) in breast tumors relative to normal tissue.

Toxicology Research: Rezulin (Troglitazone or "Tro" or "T"), a glitazone PPAR-gamma agonist, was approved for treatment of type 2 diabetes mellitus, but was withdrawn from the market due to idiosyncratic liver toxicity. Two similar drugs, Avandia (Rosiglitazone or "Rosi" or "R") and Actos (Pioglitazone or "Pio" or "P"), are considered to be safe treatments for the same condition. The expression profile of key drug metabolism genes should be different in cells treated with Rezulin versus those treated with Avandia and Actos. Hepatocellular carcinoma HepG2 cells were treated at 80% cell confluence with these three drugs (100 µM, Cayman Chemical) or a DMSO vehicle control for 24 h. RNA isolated using the ArrayGrade™ Total RNA Isolation Kit was used to characterize gene expression with the Human Drug Metabolism and Stress & Toxicity PathwayFinder™ RT² Profiler™ PCR Arrays and RT² SYBR Green / Fluorescein PCR master mix on the Bio-Rad iCycler®.



Figure 6:

Stress & Toxicity PathwayFinder™ PCR Array Uncovered Idiosyncratic Mechanisms of Action for Liver Toxicity Caused by 3 PPARγ Agonists. RNA from HepG2 cells treated with three different glitazone PPARγ agonists for type 2 diabetes mellitus was characterized, and the results were compared to that of a vehicle (DMSO) control. A withdrawn drug with idiosyncratic liver toxicity (Rezulin) induces very different changes in the expression of stress-related genes than two safer drugs still on the market (Avandia and Actos). Back to Top


How It Works

Manual & Resources

Reagents & Software

User Manual

RT² Profiler™ PCR Array User Manual (PDF)

White Paper

PCR Array: A Simple and Quantitative Method for Gene Expression Profiling (PDF) PCR Array Application Examples: Toxicology, Oncology, and Immunology Research (PDF)

Data Analysis

Free PCR Array Data Analysis Software (Web & Excel based)

Instrument Setup Instructions

Materials & Equipment Required for our PCR products PCR Array Instrument Setup Instructions & Protocol Files

FAQ

FAQ about Real-Time PCR and Troubleshooting for our PCR products

Web Seminars

Attend a live on-line seminar hosted by an Applications Scientist about PCR

Technical Brochures

PCR Array Brochure (PDF)

Primer Assay Brochure (PDF) SYBR Green Master Mix Brochure (PDF)

Powerpoint Presentations

PCR Technology Overview Presentations

Citations & Publications

Literature Citations using our PCR product

Pathway Central

Pathway reviews and presentation-ready pathway maps

shRNA Plasmids

Pathway related gene knockout - guaranteed > 70% knockdown

Individual Primers

Validated and Certified qPCR Primers for the genes on this array Back to Top


How It Works

Manual & Resources

Reagents & Software

The complete RT² Profiler™ PCR Array System insures that you will get good results from your pathway-focused gene expression analysis experiment the first time, and every time guaranteed. The Complete RT² Profiler™ PCR Array System Includes:



RT² Profiler PCR Arrays Over 100 pathways available RT² First Strand Kit Sensitive qRT-PCR with RNA Quality Control and NO Genomic DNA Worries RT² qPCR Master Mixes Instrument-specific PCR master mixes insure high-efficiency, gene-specific amplification for the most accurate realtime PCR results. PCR Array Data Analysis Software (Excel & Web based)

Our convenient and high-quality real-time RT-PCR accessory products complete the RT² Profiler™ PCR Array System and the RT² PCR Primer Assays. The other components needed for real-time PCR analysis are SYBR Green PCR master mix, first strand synthesis kit, and an optional RNA QC array. Other recommended accessory products NEW: RT² RNA QC PCR Array Tests for RNA Quality and Inhibitors of RT-PCR Analyses, optional Back to Top

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